Sewage sludge and liquid pig manure as possible sources of antibiotic resistant bacteria
Within the last decades, the environmental spread of antibiotic resistant bacteria has become a topic of concern. In this study, liquid pig manure ( n=305) and sewage sludge ( n=111) – used as agricultural fertilizers between 2002 and 2005 – were investigated for the presence of Escherichia coli, En...
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| Veröffentlicht in: | Environmental research 2010-05, Vol.110 (4), p.318-326 |
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| creator | Hölzel, Christina S. Schwaiger, Karin Harms, Katrin Küchenhoff, Helmut Kunz, Anne Meyer, Karsten Müller, Christa Bauer, Johann |
| description | Within the last decades, the environmental spread of antibiotic resistant bacteria has become a topic of concern. In this study, liquid pig manure (
n=305) and sewage sludge (
n=111) – used as agricultural fertilizers between 2002 and 2005 – were investigated for the presence of
Escherichia coli,
Enterococcus faecalis and
Enterococcus faecium. Bacteria were tested for their resistance against 40 chemotherapeutics including several “reserve drugs”.
E. coli (
n=613) from pig manure were at a significantly higher degree resistant to streptomycin, doxycycline, spectinomycin, cotrimoxazole, and chloramphenicol than
E. coli (
n=116) from sewage sludge. Enterococci (
Ent. faecalis,
n=387, and
Ent. faecium,
n=183) from pig manure were significantly more often resistant to high levels of doxycycline, rifampicin, erythromycin, and streptomycin than
Ent. faecalis (
n=44) and
Ent. faecium (
n=125) from sewage sludge. Significant differences in enterococcal resistance were also seen for tylosin, chloramphenicol, gentamicin high level, fosfomycin, clindamicin, enrofloxacin, moxifloxacin, nitrofurantoin, and quinupristin/dalfopristin
. By contrast, aminopenicillins were more effective in enterococci from pig manure, and mean MIC-values of piperacillin+tazobactam and third generation cefalosporines were significantly lower in
E. coli from pig manure than in
E. coli from sewage sludge. 13.4% (
E. coli) to 25.3% (
Ent. faecium) of pig manure isolates were high-level multiresistant to substances from more than three different classes of antimicrobial agents. In sewage sludge, high-level-multiresistance reached from 0% (
Ent. faecalis) to 16% (
Ent. faecium). High rates of (multi-) resistant bacteria in pig manure emphasize the need for a prudent – cautious – use of antibiotics in farm animals. |
| doi_str_mv | 10.1016/j.envres.2010.02.009 |
| format | Article |
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n=305) and sewage sludge (
n=111) – used as agricultural fertilizers between 2002 and 2005 – were investigated for the presence of
Escherichia coli,
Enterococcus faecalis and
Enterococcus faecium. Bacteria were tested for their resistance against 40 chemotherapeutics including several “reserve drugs”.
E. coli (
n=613) from pig manure were at a significantly higher degree resistant to streptomycin, doxycycline, spectinomycin, cotrimoxazole, and chloramphenicol than
E. coli (
n=116) from sewage sludge. Enterococci (
Ent. faecalis,
n=387, and
Ent. faecium,
n=183) from pig manure were significantly more often resistant to high levels of doxycycline, rifampicin, erythromycin, and streptomycin than
Ent. faecalis (
n=44) and
Ent. faecium (
n=125) from sewage sludge. Significant differences in enterococcal resistance were also seen for tylosin, chloramphenicol, gentamicin high level, fosfomycin, clindamicin, enrofloxacin, moxifloxacin, nitrofurantoin, and quinupristin/dalfopristin
. By contrast, aminopenicillins were more effective in enterococci from pig manure, and mean MIC-values of piperacillin+tazobactam and third generation cefalosporines were significantly lower in
E. coli from pig manure than in
E. coli from sewage sludge. 13.4% (
E. coli) to 25.3% (
Ent. faecium) of pig manure isolates were high-level multiresistant to substances from more than three different classes of antimicrobial agents. In sewage sludge, high-level-multiresistance reached from 0% (
Ent. faecalis) to 16% (
Ent. faecium). High rates of (multi-) resistant bacteria in pig manure emphasize the need for a prudent – cautious – use of antibiotics in farm animals.</description><identifier>ISSN: 0013-9351</identifier><identifier>EISSN: 1096-0953</identifier><identifier>DOI: 10.1016/j.envres.2010.02.009</identifier><identifier>PMID: 20303077</identifier><identifier>CODEN: ENVRAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antimicrobial resistance ; Bacteria ; Biological and medical sciences ; Drug Resistance, Bacterial ; Enterococcus faecalis ; Enterococcus faecalis - drug effects ; Enterococcus faecalis - genetics ; Enterococcus faecalis - isolation & purification ; Enterococcus faecium ; Enterococcus faecium - drug effects ; Enterococcus faecium - genetics ; Enterococcus faecium - isolation & purification ; Environmental Monitoring ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Farms ; Fertilizers - microbiology ; General aspects ; Liquid pig manure ; Liquids ; Manure ; Manure - microbiology ; Medical sciences ; Microbial Sensitivity Tests ; Multiresistance ; Outpatients ; Pharmacology. Drug treatments ; Sewage - microbiology ; Sewage sludge ; Streptomycin ; Swine</subject><ispartof>Environmental research, 2010-05, Vol.110 (4), p.318-326</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-f4823f972e49c2de3e39f7abfc96fd9282f874906d5419b6315f0502f918ca583</citedby><cites>FETCH-LOGICAL-c456t-f4823f972e49c2de3e39f7abfc96fd9282f874906d5419b6315f0502f918ca583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.envres.2010.02.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22823418$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20303077$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hölzel, Christina S.</creatorcontrib><creatorcontrib>Schwaiger, Karin</creatorcontrib><creatorcontrib>Harms, Katrin</creatorcontrib><creatorcontrib>Küchenhoff, Helmut</creatorcontrib><creatorcontrib>Kunz, Anne</creatorcontrib><creatorcontrib>Meyer, Karsten</creatorcontrib><creatorcontrib>Müller, Christa</creatorcontrib><creatorcontrib>Bauer, Johann</creatorcontrib><title>Sewage sludge and liquid pig manure as possible sources of antibiotic resistant bacteria</title><title>Environmental research</title><addtitle>Environ Res</addtitle><description>Within the last decades, the environmental spread of antibiotic resistant bacteria has become a topic of concern. In this study, liquid pig manure (
n=305) and sewage sludge (
n=111) – used as agricultural fertilizers between 2002 and 2005 – were investigated for the presence of
Escherichia coli,
Enterococcus faecalis and
Enterococcus faecium. Bacteria were tested for their resistance against 40 chemotherapeutics including several “reserve drugs”.
E. coli (
n=613) from pig manure were at a significantly higher degree resistant to streptomycin, doxycycline, spectinomycin, cotrimoxazole, and chloramphenicol than
E. coli (
n=116) from sewage sludge. Enterococci (
Ent. faecalis,
n=387, and
Ent. faecium,
n=183) from pig manure were significantly more often resistant to high levels of doxycycline, rifampicin, erythromycin, and streptomycin than
Ent. faecalis (
n=44) and
Ent. faecium (
n=125) from sewage sludge. Significant differences in enterococcal resistance were also seen for tylosin, chloramphenicol, gentamicin high level, fosfomycin, clindamicin, enrofloxacin, moxifloxacin, nitrofurantoin, and quinupristin/dalfopristin
. By contrast, aminopenicillins were more effective in enterococci from pig manure, and mean MIC-values of piperacillin+tazobactam and third generation cefalosporines were significantly lower in
E. coli from pig manure than in
E. coli from sewage sludge. 13.4% (
E. coli) to 25.3% (
Ent. faecium) of pig manure isolates were high-level multiresistant to substances from more than three different classes of antimicrobial agents. In sewage sludge, high-level-multiresistance reached from 0% (
Ent. faecalis) to 16% (
Ent. faecium). High rates of (multi-) resistant bacteria in pig manure emphasize the need for a prudent – cautious – use of antibiotics in farm animals.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Drug Resistance, Bacterial</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - drug effects</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecalis - isolation & purification</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - drug effects</subject><subject>Enterococcus faecium - genetics</subject><subject>Enterococcus faecium - isolation & purification</subject><subject>Environmental Monitoring</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Farms</subject><subject>Fertilizers - microbiology</subject><subject>General aspects</subject><subject>Liquid pig manure</subject><subject>Liquids</subject><subject>Manure</subject><subject>Manure - microbiology</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Multiresistance</subject><subject>Outpatients</subject><subject>Pharmacology. Drug treatments</subject><subject>Sewage - microbiology</subject><subject>Sewage sludge</subject><subject>Streptomycin</subject><subject>Swine</subject><issn>0013-9351</issn><issn>1096-0953</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVIaLZp_0EovoT04s1IsmTrUghLPwKBHJJAb0KWRkGL195Idkr-fWR2k94WHQa9PCPNPIScU1hSoPJqvcT-JWJaMsgRsCWAOiILCkqWoAQ_JgsAykvFBT0ln1Na5ysVHD6RUwY8n7pekL_3-M88YZG6yeVield04XkKrtiGp2Jj-inmNBXbIaXQdhkcpmgxFYPP8BjaMIzBFnmOkMYcFK2xI8ZgvpATb7qEX_f1jDz--vmw-lPe3v2-WV3flrYScix91TDuVc2wUpY55MiVr03rrZLeKdYw39SVAulERVUrORUeBDCvaGONaPgZudy9u43D84Rp1JuQLHad6XGYkq4rCcBZIzP5_SBJaw4gpJAzWu1QG_PeEb3exrAx8VVT0LN9vdY7-3q2r4HpbD-3fdv_MLUbdB9N77ozcLEHTLKm89H0NqT_XF6XV3Re6seOw2zuJWDUyQbsLboQ0Y7aDeHwJG-t7KRG</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Hölzel, Christina S.</creator><creator>Schwaiger, Karin</creator><creator>Harms, Katrin</creator><creator>Küchenhoff, Helmut</creator><creator>Kunz, Anne</creator><creator>Meyer, Karsten</creator><creator>Müller, Christa</creator><creator>Bauer, Johann</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope><scope>7QH</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7UA</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20100501</creationdate><title>Sewage sludge and liquid pig manure as possible sources of antibiotic resistant bacteria</title><author>Hölzel, Christina S. ; Schwaiger, Karin ; Harms, Katrin ; Küchenhoff, Helmut ; Kunz, Anne ; Meyer, Karsten ; Müller, Christa ; Bauer, Johann</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-f4823f972e49c2de3e39f7abfc96fd9282f874906d5419b6315f0502f918ca583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antimicrobial resistance</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Drug Resistance, Bacterial</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - drug effects</topic><topic>Enterococcus faecalis - genetics</topic><topic>Enterococcus faecalis - isolation & purification</topic><topic>Enterococcus faecium</topic><topic>Enterococcus faecium - drug effects</topic><topic>Enterococcus faecium - genetics</topic><topic>Enterococcus faecium - isolation & purification</topic><topic>Environmental Monitoring</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Farms</topic><topic>Fertilizers - microbiology</topic><topic>General aspects</topic><topic>Liquid pig manure</topic><topic>Liquids</topic><topic>Manure</topic><topic>Manure - microbiology</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Multiresistance</topic><topic>Outpatients</topic><topic>Pharmacology. Drug treatments</topic><topic>Sewage - microbiology</topic><topic>Sewage sludge</topic><topic>Streptomycin</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hölzel, Christina S.</creatorcontrib><creatorcontrib>Schwaiger, Karin</creatorcontrib><creatorcontrib>Harms, Katrin</creatorcontrib><creatorcontrib>Küchenhoff, Helmut</creatorcontrib><creatorcontrib>Kunz, Anne</creatorcontrib><creatorcontrib>Meyer, Karsten</creatorcontrib><creatorcontrib>Müller, Christa</creatorcontrib><creatorcontrib>Bauer, Johann</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environmental Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hölzel, Christina S.</au><au>Schwaiger, Karin</au><au>Harms, Katrin</au><au>Küchenhoff, Helmut</au><au>Kunz, Anne</au><au>Meyer, Karsten</au><au>Müller, Christa</au><au>Bauer, Johann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sewage sludge and liquid pig manure as possible sources of antibiotic resistant bacteria</atitle><jtitle>Environmental research</jtitle><addtitle>Environ Res</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>110</volume><issue>4</issue><spage>318</spage><epage>326</epage><pages>318-326</pages><issn>0013-9351</issn><eissn>1096-0953</eissn><coden>ENVRAL</coden><abstract>Within the last decades, the environmental spread of antibiotic resistant bacteria has become a topic of concern. In this study, liquid pig manure (
n=305) and sewage sludge (
n=111) – used as agricultural fertilizers between 2002 and 2005 – were investigated for the presence of
Escherichia coli,
Enterococcus faecalis and
Enterococcus faecium. Bacteria were tested for their resistance against 40 chemotherapeutics including several “reserve drugs”.
E. coli (
n=613) from pig manure were at a significantly higher degree resistant to streptomycin, doxycycline, spectinomycin, cotrimoxazole, and chloramphenicol than
E. coli (
n=116) from sewage sludge. Enterococci (
Ent. faecalis,
n=387, and
Ent. faecium,
n=183) from pig manure were significantly more often resistant to high levels of doxycycline, rifampicin, erythromycin, and streptomycin than
Ent. faecalis (
n=44) and
Ent. faecium (
n=125) from sewage sludge. Significant differences in enterococcal resistance were also seen for tylosin, chloramphenicol, gentamicin high level, fosfomycin, clindamicin, enrofloxacin, moxifloxacin, nitrofurantoin, and quinupristin/dalfopristin
. By contrast, aminopenicillins were more effective in enterococci from pig manure, and mean MIC-values of piperacillin+tazobactam and third generation cefalosporines were significantly lower in
E. coli from pig manure than in
E. coli from sewage sludge. 13.4% (
E. coli) to 25.3% (
Ent. faecium) of pig manure isolates were high-level multiresistant to substances from more than three different classes of antimicrobial agents. In sewage sludge, high-level-multiresistance reached from 0% (
Ent. faecalis) to 16% (
Ent. faecium). High rates of (multi-) resistant bacteria in pig manure emphasize the need for a prudent – cautious – use of antibiotics in farm animals.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20303077</pmid><doi>10.1016/j.envres.2010.02.009</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Environmental research, 2010-05, Vol.110 (4), p.318-326 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Antibiotics. Antiinfectious agents. Antiparasitic agents Antimicrobial resistance Bacteria Biological and medical sciences Drug Resistance, Bacterial Enterococcus faecalis Enterococcus faecalis - drug effects Enterococcus faecalis - genetics Enterococcus faecalis - isolation & purification Enterococcus faecium Enterococcus faecium - drug effects Enterococcus faecium - genetics Enterococcus faecium - isolation & purification Environmental Monitoring Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - isolation & purification Farms Fertilizers - microbiology General aspects Liquid pig manure Liquids Manure Manure - microbiology Medical sciences Microbial Sensitivity Tests Multiresistance Outpatients Pharmacology. Drug treatments Sewage - microbiology Sewage sludge Streptomycin Swine |
| title | Sewage sludge and liquid pig manure as possible sources of antibiotic resistant bacteria |
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