c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response
The tyrosine kinase, c-Abl, plays important roles in many aspects of cellular function. Previous reports showed that c-Abl is involved in NF-κB signaling. However, the functions of c-Abl in innate immunity are still unknown. Here we demonstrate that the mitochondrial antiviral signaling (MAVS) prote...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 2010-01, Vol.584 (1), p.33-38 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 38 |
|---|---|
| container_issue | 1 |
| container_start_page | 33 |
| container_title | FEBS letters |
| container_volume | 584 |
| creator | Song, Ting Wei, Congwen Zheng, Zirui Xu, Yang Cheng, Xiaozhong Yuan, Yuan Guan, Kai Zhang, Yanhong Ma, Qingjun Shi, Wei Zhong, Hui |
| description | The tyrosine kinase, c-Abl, plays important roles in many aspects of cellular function. Previous reports showed that c-Abl is involved in NF-κB signaling. However, the functions of c-Abl in innate immunity are still unknown. Here we demonstrate that the mitochondrial antiviral signaling (MAVS) protein can be physically associated with c-Abl in vivo and in vitro. MAVS interacted with c-Abl through its Card and TM domain. A phosphotyrosine-specific antibody indicated that MAVS was phosphorylated by c-Abl. Functional impairment of c-Abl attenuated MAVS or VSV induced type-I IFN production. Importantly, c-Abl knockdown in MCF7 cells displayed impaired MAVS-mediated NF-κB and IRF3 activation. Taken together, our results suggest that c-Abl modulates innate immune response through MAVS.
MINT-
7297498, MINT-
7297511, MINT-
7297557, MINT-
7297574:
MAVS (uniprotkb:
Q7Z434)
physically interacts (MI:
0915) with
c-Abl (uniprotkb:
P00519) by
anti tag coimmunoprecipitation (MI:
0007)MINT-
7297542:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
anti bait coimmunoprecipitation (MI:
0006)MINT-
7297526:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
far western blotting (MI:
0047) |
| doi_str_mv | 10.1016/j.febslet.2009.11.025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_746001844</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579309009272</els_id><sourcerecordid>734195573</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5082-da391b0437a31fcebb0fea0a40bdd98b8f4d5387b170c7e484f2f8f4c516ee2a3</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi0EotvCTwDlxinB4491fEJL1dJKLT20cLVsZwJe8rHYSav99zjalTi2p5HtZ17bzxDyAWgFFNaft1WLLnU4VYxSXQFUlMlXZAW14iUX6_o1WVEKopRK8xNymtKW5nUN-i05Aa1BMCFX5LsvN64rpn0cUxiw-BMGm7AIw4TR-ikVT2H6Xdxuft4XdmiKiL_mzk6YMjHkWoS-n3NbxLQbh4TvyJvWdgnfH-sZ-XF58XB-Vd7cfbs-39yUXtKalY3lGhwVXFkOrUfnaIuWWkFd0-ja1a1oJK-VA0W9QlGLlrV500tYIzLLz8inQ-4ujn9nTJPpQ_LYdXbAcU5GifXyWSGeJ7kALaXimZQH0mcXKWJrdjH0Nu4NULM4N1tzdG4W5wbAZOe57-Pxhtn12PzvOkrOwNUBeAod7l-Wai4vvrL7ZYDL_KjOJ0yxHPXlEIVZ7mPAaJIPOHhsQkQ_mWYMz7z2H6rYquU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734195573</pqid></control><display><type>article</type><title>c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response</title><source>MEDLINE</source><source>Wiley Journals</source><source>Elsevier ScienceDirect Journals Complete</source><source>Wiley Online Library Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Song, Ting ; Wei, Congwen ; Zheng, Zirui ; Xu, Yang ; Cheng, Xiaozhong ; Yuan, Yuan ; Guan, Kai ; Zhang, Yanhong ; Ma, Qingjun ; Shi, Wei ; Zhong, Hui</creator><creatorcontrib>Song, Ting ; Wei, Congwen ; Zheng, Zirui ; Xu, Yang ; Cheng, Xiaozhong ; Yuan, Yuan ; Guan, Kai ; Zhang, Yanhong ; Ma, Qingjun ; Shi, Wei ; Zhong, Hui</creatorcontrib><description>The tyrosine kinase, c-Abl, plays important roles in many aspects of cellular function. Previous reports showed that c-Abl is involved in NF-κB signaling. However, the functions of c-Abl in innate immunity are still unknown. Here we demonstrate that the mitochondrial antiviral signaling (MAVS) protein can be physically associated with c-Abl in vivo and in vitro. MAVS interacted with c-Abl through its Card and TM domain. A phosphotyrosine-specific antibody indicated that MAVS was phosphorylated by c-Abl. Functional impairment of c-Abl attenuated MAVS or VSV induced type-I IFN production. Importantly, c-Abl knockdown in MCF7 cells displayed impaired MAVS-mediated NF-κB and IRF3 activation. Taken together, our results suggest that c-Abl modulates innate immune response through MAVS.
MINT-
7297498, MINT-
7297511, MINT-
7297557, MINT-
7297574:
MAVS (uniprotkb:
Q7Z434)
physically interacts (MI:
0915) with
c-Abl (uniprotkb:
P00519) by
anti tag coimmunoprecipitation (MI:
0007)MINT-
7297542:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
anti bait coimmunoprecipitation (MI:
0006)MINT-
7297526:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
far western blotting (MI:
0047)</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2009.11.025</identifier><identifier>PMID: 19914245</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Adaptor Proteins, Signal Transducing - metabolism ; c-Abl non-receptor tyrosine kinase ; Cell Line ; Humans ; Immunity, Innate ; Innate immune response ; Interferon Regulatory Factor-3 - biosynthesis ; Interferon-beta - biosynthesis ; Mitochondrial antiviral signaling ; NF-kappa B - biosynthesis ; Phosphorylation ; Protein Interaction Domains and Motifs ; Protein Interaction Mapping ; Proto-Oncogene Proteins c-abl - genetics ; Proto-Oncogene Proteins c-abl - metabolism ; RIG1 signaling</subject><ispartof>FEBS letters, 2010-01, Vol.584 (1), p.33-38</ispartof><rights>2009 Federation of European Biochemical Societies</rights><rights>FEBS Letters 584 (2010) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5082-da391b0437a31fcebb0fea0a40bdd98b8f4d5387b170c7e484f2f8f4c516ee2a3</citedby><cites>FETCH-LOGICAL-c5082-da391b0437a31fcebb0fea0a40bdd98b8f4d5387b170c7e484f2f8f4c516ee2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2009.11.025$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2009.11.025$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19914245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Ting</creatorcontrib><creatorcontrib>Wei, Congwen</creatorcontrib><creatorcontrib>Zheng, Zirui</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Cheng, Xiaozhong</creatorcontrib><creatorcontrib>Yuan, Yuan</creatorcontrib><creatorcontrib>Guan, Kai</creatorcontrib><creatorcontrib>Zhang, Yanhong</creatorcontrib><creatorcontrib>Ma, Qingjun</creatorcontrib><creatorcontrib>Shi, Wei</creatorcontrib><creatorcontrib>Zhong, Hui</creatorcontrib><title>c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The tyrosine kinase, c-Abl, plays important roles in many aspects of cellular function. Previous reports showed that c-Abl is involved in NF-κB signaling. However, the functions of c-Abl in innate immunity are still unknown. Here we demonstrate that the mitochondrial antiviral signaling (MAVS) protein can be physically associated with c-Abl in vivo and in vitro. MAVS interacted with c-Abl through its Card and TM domain. A phosphotyrosine-specific antibody indicated that MAVS was phosphorylated by c-Abl. Functional impairment of c-Abl attenuated MAVS or VSV induced type-I IFN production. Importantly, c-Abl knockdown in MCF7 cells displayed impaired MAVS-mediated NF-κB and IRF3 activation. Taken together, our results suggest that c-Abl modulates innate immune response through MAVS.
MINT-
7297498, MINT-
7297511, MINT-
7297557, MINT-
7297574:
MAVS (uniprotkb:
Q7Z434)
physically interacts (MI:
0915) with
c-Abl (uniprotkb:
P00519) by
anti tag coimmunoprecipitation (MI:
0007)MINT-
7297542:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
anti bait coimmunoprecipitation (MI:
0006)MINT-
7297526:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
far western blotting (MI:
0047)</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>c-Abl non-receptor tyrosine kinase</subject><subject>Cell Line</subject><subject>Humans</subject><subject>Immunity, Innate</subject><subject>Innate immune response</subject><subject>Interferon Regulatory Factor-3 - biosynthesis</subject><subject>Interferon-beta - biosynthesis</subject><subject>Mitochondrial antiviral signaling</subject><subject>NF-kappa B - biosynthesis</subject><subject>Phosphorylation</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Protein Interaction Mapping</subject><subject>Proto-Oncogene Proteins c-abl - genetics</subject><subject>Proto-Oncogene Proteins c-abl - metabolism</subject><subject>RIG1 signaling</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCTwDlxinB4491fEJL1dJKLT20cLVsZwJe8rHYSav99zjalTi2p5HtZ17bzxDyAWgFFNaft1WLLnU4VYxSXQFUlMlXZAW14iUX6_o1WVEKopRK8xNymtKW5nUN-i05Aa1BMCFX5LsvN64rpn0cUxiw-BMGm7AIw4TR-ikVT2H6Xdxuft4XdmiKiL_mzk6YMjHkWoS-n3NbxLQbh4TvyJvWdgnfH-sZ-XF58XB-Vd7cfbs-39yUXtKalY3lGhwVXFkOrUfnaIuWWkFd0-ja1a1oJK-VA0W9QlGLlrV500tYIzLLz8inQ-4ujn9nTJPpQ_LYdXbAcU5GifXyWSGeJ7kALaXimZQH0mcXKWJrdjH0Nu4NULM4N1tzdG4W5wbAZOe57-Pxhtn12PzvOkrOwNUBeAod7l-Wai4vvrL7ZYDL_KjOJ0yxHPXlEIVZ7mPAaJIPOHhsQkQ_mWYMz7z2H6rYquU</recordid><startdate>20100104</startdate><enddate>20100104</enddate><creator>Song, Ting</creator><creator>Wei, Congwen</creator><creator>Zheng, Zirui</creator><creator>Xu, Yang</creator><creator>Cheng, Xiaozhong</creator><creator>Yuan, Yuan</creator><creator>Guan, Kai</creator><creator>Zhang, Yanhong</creator><creator>Ma, Qingjun</creator><creator>Shi, Wei</creator><creator>Zhong, Hui</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20100104</creationdate><title>c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response</title><author>Song, Ting ; Wei, Congwen ; Zheng, Zirui ; Xu, Yang ; Cheng, Xiaozhong ; Yuan, Yuan ; Guan, Kai ; Zhang, Yanhong ; Ma, Qingjun ; Shi, Wei ; Zhong, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5082-da391b0437a31fcebb0fea0a40bdd98b8f4d5387b170c7e484f2f8f4c516ee2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>c-Abl non-receptor tyrosine kinase</topic><topic>Cell Line</topic><topic>Humans</topic><topic>Immunity, Innate</topic><topic>Innate immune response</topic><topic>Interferon Regulatory Factor-3 - biosynthesis</topic><topic>Interferon-beta - biosynthesis</topic><topic>Mitochondrial antiviral signaling</topic><topic>NF-kappa B - biosynthesis</topic><topic>Phosphorylation</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Protein Interaction Mapping</topic><topic>Proto-Oncogene Proteins c-abl - genetics</topic><topic>Proto-Oncogene Proteins c-abl - metabolism</topic><topic>RIG1 signaling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Ting</creatorcontrib><creatorcontrib>Wei, Congwen</creatorcontrib><creatorcontrib>Zheng, Zirui</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Cheng, Xiaozhong</creatorcontrib><creatorcontrib>Yuan, Yuan</creatorcontrib><creatorcontrib>Guan, Kai</creatorcontrib><creatorcontrib>Zhang, Yanhong</creatorcontrib><creatorcontrib>Ma, Qingjun</creatorcontrib><creatorcontrib>Shi, Wei</creatorcontrib><creatorcontrib>Zhong, Hui</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Ting</au><au>Wei, Congwen</au><au>Zheng, Zirui</au><au>Xu, Yang</au><au>Cheng, Xiaozhong</au><au>Yuan, Yuan</au><au>Guan, Kai</au><au>Zhang, Yanhong</au><au>Ma, Qingjun</au><au>Shi, Wei</au><au>Zhong, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2010-01-04</date><risdate>2010</risdate><volume>584</volume><issue>1</issue><spage>33</spage><epage>38</epage><pages>33-38</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The tyrosine kinase, c-Abl, plays important roles in many aspects of cellular function. Previous reports showed that c-Abl is involved in NF-κB signaling. However, the functions of c-Abl in innate immunity are still unknown. Here we demonstrate that the mitochondrial antiviral signaling (MAVS) protein can be physically associated with c-Abl in vivo and in vitro. MAVS interacted with c-Abl through its Card and TM domain. A phosphotyrosine-specific antibody indicated that MAVS was phosphorylated by c-Abl. Functional impairment of c-Abl attenuated MAVS or VSV induced type-I IFN production. Importantly, c-Abl knockdown in MCF7 cells displayed impaired MAVS-mediated NF-κB and IRF3 activation. Taken together, our results suggest that c-Abl modulates innate immune response through MAVS.
MINT-
7297498, MINT-
7297511, MINT-
7297557, MINT-
7297574:
MAVS (uniprotkb:
Q7Z434)
physically interacts (MI:
0915) with
c-Abl (uniprotkb:
P00519) by
anti tag coimmunoprecipitation (MI:
0007)MINT-
7297542:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
anti bait coimmunoprecipitation (MI:
0006)MINT-
7297526:
c-Abl (uniprotkb:
P00519)
physically interacts (MI:
0915) with
MAVS (uniprotkb:
Q7Z434) by
far western blotting (MI:
0047)</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>19914245</pmid><doi>10.1016/j.febslet.2009.11.025</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2010-01, Vol.584 (1), p.33-38 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_746001844 |
| source | MEDLINE; Wiley Journals; Elsevier ScienceDirect Journals Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism c-Abl non-receptor tyrosine kinase Cell Line Humans Immunity, Innate Innate immune response Interferon Regulatory Factor-3 - biosynthesis Interferon-beta - biosynthesis Mitochondrial antiviral signaling NF-kappa B - biosynthesis Phosphorylation Protein Interaction Domains and Motifs Protein Interaction Mapping Proto-Oncogene Proteins c-abl - genetics Proto-Oncogene Proteins c-abl - metabolism RIG1 signaling |
| title | c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T14%3A35%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=c-Abl%20tyrosine%20kinase%20interacts%20with%20MAVS%20and%20regulates%20innate%20immune%20response&rft.jtitle=FEBS%20letters&rft.au=Song,%20Ting&rft.date=2010-01-04&rft.volume=584&rft.issue=1&rft.spage=33&rft.epage=38&rft.pages=33-38&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/j.febslet.2009.11.025&rft_dat=%3Cproquest_cross%3E734195573%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=734195573&rft_id=info:pmid/19914245&rft_els_id=S0014579309009272&rfr_iscdi=true |