Assay of Chlamydia pneumoniae-Specific IgM Antibodies by ELISA Method―Reduction of Non-Specific Reaction and Resetting of Serological Criteria by Measuring IgM Antibodies
In the present study, we tried to reduce the non-specific reactions for measuring anti-Chlamydia pneumoniae IgM antibodies by the ELISA kit of HITAZYME C. pneumoniae Ab-IgM (HITAZYME IgM) by using a new absorbent. We also tried to reset the IgM cut-off index (ID) of HITAZYME IgM by testing serum sam...
Gespeichert in:
| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2009/07/29, Vol.62(4), pp.260-264 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 264 |
|---|---|
| container_issue | 4 |
| container_start_page | 260 |
| container_title | Japanese Journal of Infectious Diseases |
| container_volume | 62 |
| creator | Kishimoto, Toshio Ando, Shuji Numazaki, Kei Ouchi, Kazunobu Yamazaki, Tsutomu Nakahama, Chikara |
| description | In the present study, we tried to reduce the non-specific reactions for measuring anti-Chlamydia pneumoniae IgM antibodies by the ELISA kit of HITAZYME C. pneumoniae Ab-IgM (HITAZYME IgM) by using a new absorbent. We also tried to reset the IgM cut-off index (ID) of HITAZYME IgM by testing serum samples from healthy children and healthy adults with no respiratory symptoms. The results suggest that the use of the new absorbent (anti-human IgG antibodies) may reduce the non-specific reactions by rheumatoid factor and anti-nuclear antibodies, and that the setting of the higher cut-off ID (2.00), calculated as the mean ID + 3SD of the serum samples from healthy children and healthy adults, respectively, would improve the specificity of IgM during the measurement by HITAZYME IgM. |
| doi_str_mv | 10.7883/yoken.JJID.2009.260 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_745977417</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67516565</sourcerecordid><originalsourceid>FETCH-LOGICAL-c574t-60d604e780c1028534af0072b33184e89e30c97aa4d3da2f05e13510606e82c93</originalsourceid><addsrcrecordid>eNp9kU1u1DAYhiMEoqVwAiSUFawy-C-OsxwNpUw1A1IH1pbH-TLjkthT21lkxyV6C07FSXCYUatu2PhHft73k_xk2VuMZpUQ9OPofoKdXV8vP80IQvWMcPQsO8dCsIIIyp-nM2Ws4BSxs-xVCLcIkbLE6GV2hmtORI3wefZ7HoIac9fmi32n-rExKj9YGHpnjYJicwBtWqPz5W6dz200W9cYCPl2zC9Xy808X0Pcu-bPr_sbaAYdjbNT11dnH6M3oI4PyjbpEiBGY3cTtgHvOrczWnX5wpsIPk1P1WtQYfAT9HTs6-xFq7oAb077Rfbj8-X3xZdi9e1quZivCl1WLBYcNRwxqATSGBFRUqZahCqypRQLBqIGinRdKcUa2ijSohIwTR_DEQdBdE0vsg_H3oN3dwOEKHsTNHSdsuCGICtW1lXFcJXI9_8leVViXvIygfQIau9C8NDKgze98qPESE465T-dctIpJ50y6Uypd6f6YdtD85g5-UvA8gjchqh28AAoH43u4FTKiWTT8qT8gdF75SVY-hdKELkR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67516565</pqid></control><display><type>article</type><title>Assay of Chlamydia pneumoniae-Specific IgM Antibodies by ELISA Method―Reduction of Non-Specific Reaction and Resetting of Serological Criteria by Measuring IgM Antibodies</title><source>J-STAGE Free</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Kishimoto, Toshio ; Ando, Shuji ; Numazaki, Kei ; Ouchi, Kazunobu ; Yamazaki, Tsutomu ; Nakahama, Chikara</creator><creatorcontrib>Kishimoto, Toshio ; Ando, Shuji ; Numazaki, Kei ; Ouchi, Kazunobu ; Yamazaki, Tsutomu ; Nakahama, Chikara</creatorcontrib><description>In the present study, we tried to reduce the non-specific reactions for measuring anti-Chlamydia pneumoniae IgM antibodies by the ELISA kit of HITAZYME C. pneumoniae Ab-IgM (HITAZYME IgM) by using a new absorbent. We also tried to reset the IgM cut-off index (ID) of HITAZYME IgM by testing serum samples from healthy children and healthy adults with no respiratory symptoms. The results suggest that the use of the new absorbent (anti-human IgG antibodies) may reduce the non-specific reactions by rheumatoid factor and anti-nuclear antibodies, and that the setting of the higher cut-off ID (2.00), calculated as the mean ID + 3SD of the serum samples from healthy children and healthy adults, respectively, would improve the specificity of IgM during the measurement by HITAZYME IgM.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.JJID.2009.260</identifier><identifier>PMID: 19628901</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Bacterial - blood ; Child ; Child, Preschool ; Children ; Chlamydia ; Chlamydophila Infections - diagnosis ; Chlamydophila pneumoniae ; Chlamydophila pneumoniae - immunology ; Chlamydophila pneumoniae - isolation & purification ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Immunoglobulin M - blood ; Infant ; Infant, Newborn ; Male ; Middle Aged ; Rheumatoid factor ; Sensitivity and Specificity ; Young Adult</subject><ispartof>Japanese Journal of Infectious Diseases, 2009/07/29, Vol.62(4), pp.260-264</ispartof><rights>2009 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-60d604e780c1028534af0072b33184e89e30c97aa4d3da2f05e13510606e82c93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19628901$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kishimoto, Toshio</creatorcontrib><creatorcontrib>Ando, Shuji</creatorcontrib><creatorcontrib>Numazaki, Kei</creatorcontrib><creatorcontrib>Ouchi, Kazunobu</creatorcontrib><creatorcontrib>Yamazaki, Tsutomu</creatorcontrib><creatorcontrib>Nakahama, Chikara</creatorcontrib><title>Assay of Chlamydia pneumoniae-Specific IgM Antibodies by ELISA Method―Reduction of Non-Specific Reaction and Resetting of Serological Criteria by Measuring IgM Antibodies</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>In the present study, we tried to reduce the non-specific reactions for measuring anti-Chlamydia pneumoniae IgM antibodies by the ELISA kit of HITAZYME C. pneumoniae Ab-IgM (HITAZYME IgM) by using a new absorbent. We also tried to reset the IgM cut-off index (ID) of HITAZYME IgM by testing serum samples from healthy children and healthy adults with no respiratory symptoms. The results suggest that the use of the new absorbent (anti-human IgG antibodies) may reduce the non-specific reactions by rheumatoid factor and anti-nuclear antibodies, and that the setting of the higher cut-off ID (2.00), calculated as the mean ID + 3SD of the serum samples from healthy children and healthy adults, respectively, would improve the specificity of IgM during the measurement by HITAZYME IgM.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies, Bacterial - blood</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Chlamydia</subject><subject>Chlamydophila Infections - diagnosis</subject><subject>Chlamydophila pneumoniae</subject><subject>Chlamydophila pneumoniae - immunology</subject><subject>Chlamydophila pneumoniae - isolation & purification</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulin M - blood</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Rheumatoid factor</subject><subject>Sensitivity and Specificity</subject><subject>Young Adult</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1u1DAYhiMEoqVwAiSUFawy-C-OsxwNpUw1A1IH1pbH-TLjkthT21lkxyV6C07FSXCYUatu2PhHft73k_xk2VuMZpUQ9OPofoKdXV8vP80IQvWMcPQsO8dCsIIIyp-nM2Ws4BSxs-xVCLcIkbLE6GV2hmtORI3wefZ7HoIac9fmi32n-rExKj9YGHpnjYJicwBtWqPz5W6dz200W9cYCPl2zC9Xy808X0Pcu-bPr_sbaAYdjbNT11dnH6M3oI4PyjbpEiBGY3cTtgHvOrczWnX5wpsIPk1P1WtQYfAT9HTs6-xFq7oAb077Rfbj8-X3xZdi9e1quZivCl1WLBYcNRwxqATSGBFRUqZahCqypRQLBqIGinRdKcUa2ijSohIwTR_DEQdBdE0vsg_H3oN3dwOEKHsTNHSdsuCGICtW1lXFcJXI9_8leVViXvIygfQIau9C8NDKgze98qPESE465T-dctIpJ50y6Uypd6f6YdtD85g5-UvA8gjchqh28AAoH43u4FTKiWTT8qT8gdF75SVY-hdKELkR</recordid><startdate>20090729</startdate><enddate>20090729</enddate><creator>Kishimoto, Toshio</creator><creator>Ando, Shuji</creator><creator>Numazaki, Kei</creator><creator>Ouchi, Kazunobu</creator><creator>Yamazaki, Tsutomu</creator><creator>Nakahama, Chikara</creator><general>National Institute of Infectious Diseases</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20090729</creationdate><title>Assay of Chlamydia pneumoniae-Specific IgM Antibodies by ELISA Method―Reduction of Non-Specific Reaction and Resetting of Serological Criteria by Measuring IgM Antibodies</title><author>Kishimoto, Toshio ; Ando, Shuji ; Numazaki, Kei ; Ouchi, Kazunobu ; Yamazaki, Tsutomu ; Nakahama, Chikara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-60d604e780c1028534af0072b33184e89e30c97aa4d3da2f05e13510606e82c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antibodies, Bacterial - blood</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Chlamydia</topic><topic>Chlamydophila Infections - diagnosis</topic><topic>Chlamydophila pneumoniae</topic><topic>Chlamydophila pneumoniae - immunology</topic><topic>Chlamydophila pneumoniae - isolation & purification</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin M</topic><topic>Immunoglobulin M - blood</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Rheumatoid factor</topic><topic>Sensitivity and Specificity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishimoto, Toshio</creatorcontrib><creatorcontrib>Ando, Shuji</creatorcontrib><creatorcontrib>Numazaki, Kei</creatorcontrib><creatorcontrib>Ouchi, Kazunobu</creatorcontrib><creatorcontrib>Yamazaki, Tsutomu</creatorcontrib><creatorcontrib>Nakahama, Chikara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Japanese Journal of Infectious Diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishimoto, Toshio</au><au>Ando, Shuji</au><au>Numazaki, Kei</au><au>Ouchi, Kazunobu</au><au>Yamazaki, Tsutomu</au><au>Nakahama, Chikara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assay of Chlamydia pneumoniae-Specific IgM Antibodies by ELISA Method―Reduction of Non-Specific Reaction and Resetting of Serological Criteria by Measuring IgM Antibodies</atitle><jtitle>Japanese Journal of Infectious Diseases</jtitle><addtitle>Jpn J Infect Dis</addtitle><date>2009-07-29</date><risdate>2009</risdate><volume>62</volume><issue>4</issue><spage>260</spage><epage>264</epage><pages>260-264</pages><artnum>JJID.2009.260</artnum><issn>1344-6304</issn><eissn>1884-2836</eissn><abstract>In the present study, we tried to reduce the non-specific reactions for measuring anti-Chlamydia pneumoniae IgM antibodies by the ELISA kit of HITAZYME C. pneumoniae Ab-IgM (HITAZYME IgM) by using a new absorbent. We also tried to reset the IgM cut-off index (ID) of HITAZYME IgM by testing serum samples from healthy children and healthy adults with no respiratory symptoms. The results suggest that the use of the new absorbent (anti-human IgG antibodies) may reduce the non-specific reactions by rheumatoid factor and anti-nuclear antibodies, and that the setting of the higher cut-off ID (2.00), calculated as the mean ID + 3SD of the serum samples from healthy children and healthy adults, respectively, would improve the specificity of IgM during the measurement by HITAZYME IgM.</abstract><cop>Japan</cop><pub>National Institute of Infectious Diseases</pub><pmid>19628901</pmid><doi>10.7883/yoken.JJID.2009.260</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1344-6304 |
| ispartof | Japanese Journal of Infectious Diseases, 2009/07/29, Vol.62(4), pp.260-264 |
| issn | 1344-6304 1884-2836 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_745977417 |
| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Aged Aged, 80 and over Antibodies, Bacterial - blood Child Child, Preschool Children Chlamydia Chlamydophila Infections - diagnosis Chlamydophila pneumoniae Chlamydophila pneumoniae - immunology Chlamydophila pneumoniae - isolation & purification Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Female Humans Immunoglobulin G Immunoglobulin M Immunoglobulin M - blood Infant Infant, Newborn Male Middle Aged Rheumatoid factor Sensitivity and Specificity Young Adult |
| title | Assay of Chlamydia pneumoniae-Specific IgM Antibodies by ELISA Method―Reduction of Non-Specific Reaction and Resetting of Serological Criteria by Measuring IgM Antibodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T13%3A53%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assay%20of%20Chlamydia%20pneumoniae-Specific%20IgM%20Antibodies%20by%20ELISA%20Method%E2%80%95Reduction%20of%20Non-Specific%20Reaction%20and%20Resetting%20of%20Serological%20Criteria%20by%20Measuring%20IgM%20Antibodies&rft.jtitle=Japanese%20Journal%20of%20Infectious%20Diseases&rft.au=Kishimoto,%20Toshio&rft.date=2009-07-29&rft.volume=62&rft.issue=4&rft.spage=260&rft.epage=264&rft.pages=260-264&rft.artnum=JJID.2009.260&rft.issn=1344-6304&rft.eissn=1884-2836&rft_id=info:doi/10.7883/yoken.JJID.2009.260&rft_dat=%3Cproquest_cross%3E67516565%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67516565&rft_id=info:pmid/19628901&rfr_iscdi=true |