Gm-TX, a new toxic protein from soybean ( Glycine max) seeds with potential for controlling insect pests
A novel toxic protein from Glycine max seeds, named Gm-TX, was purified by combination of differential precipitation with ammonium sulphate, DEAE-cellulose, anhydrotrypsin-sepharose 4B and Superdex 200 HR fast-protein liquid chromatography. Gm-TX is composed of one polypeptide chain of 28 kDa (SDS-P...
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| Veröffentlicht in: | Process biochemistry (1991) 2010-05, Vol.45 (5), p.634-640 |
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| container_title | Process biochemistry (1991) |
| container_volume | 45 |
| creator | Oliveira, Hermogenes D. Sousa, Daniele O.B. Oliveira, Jose T.A. Carlini, Celia R. Oliveira, Henrique P. Pereira, Mirella L. Rocha, Raquel O. Morais, Janne K.S. Gomes-Filho, Eneas Vasconcelos, Ilka M. |
| description | A novel toxic protein from
Glycine max seeds, named
Gm-TX, was purified by combination of differential precipitation with ammonium sulphate, DEAE-cellulose, anhydrotrypsin-sepharose 4B and Superdex 200 HR fast-protein liquid chromatography.
Gm-TX is composed of one polypeptide chain of 28
kDa (SDS-PAGE), with p
I 5.1–5.2, contains |
| doi_str_mv | 10.1016/j.procbio.2009.12.012 |
| format | Article |
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Glycine max seeds, named
Gm-TX, was purified by combination of differential precipitation with ammonium sulphate, DEAE-cellulose, anhydrotrypsin-sepharose 4B and Superdex 200 HR fast-protein liquid chromatography.
Gm-TX is composed of one polypeptide chain of 28
kDa (SDS-PAGE), with p
I 5.1–5.2, contains <0.5% neutral sugar, has N-terminal sequence comprised of KTISSEDSPFFNCREK, maximum absorption spectrum at 280
nm and molar extinction coefficient of 16.9. It exhibited ribonuclease activity (1821.42
±
3.34
UA/h/mgP) toward yeast RNA and promoted rabbit erythrocyte agglutination (120.4
HU/mgP) mediated by anti-
Gm-TX IgG, which was inhibited by mannose. It was devoid of trypsin inhibitory, urease and chitinase activities.
Gm-TX was lethal to mice by intraperitoneal route with LD
50 of 4.5
mg/kg. Moreover, it showed dose-dependent insecticidal activity on the major economically important pests
Callosobruchus maculatus and
Dysdercus peruvianus, producing ca. 50% and 56% mortality when incorporated into artificial diets at a level of 1.16% and 0.25%, respectively. These data indicate that
Gm-TX is a candidate protein that could be expressed in genetically transformed plants for improved resistance to insect pests.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2009.12.012</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Biocontrol ; Callosobruchus maculatus ; Dysdercus peruvianus ; Glycine max ; Insecticidal activity ; Plant defence ; Plant protein ; Soybean</subject><ispartof>Process biochemistry (1991), 2010-05, Vol.45 (5), p.634-640</ispartof><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-9b371166d61aae75ff1cf27088ec0f620f1c851bc2d226af7419c0ce86bfe6883</citedby><cites>FETCH-LOGICAL-c341t-9b371166d61aae75ff1cf27088ec0f620f1c851bc2d226af7419c0ce86bfe6883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.procbio.2009.12.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids></links><search><creatorcontrib>Oliveira, Hermogenes D.</creatorcontrib><creatorcontrib>Sousa, Daniele O.B.</creatorcontrib><creatorcontrib>Oliveira, Jose T.A.</creatorcontrib><creatorcontrib>Carlini, Celia R.</creatorcontrib><creatorcontrib>Oliveira, Henrique P.</creatorcontrib><creatorcontrib>Pereira, Mirella L.</creatorcontrib><creatorcontrib>Rocha, Raquel O.</creatorcontrib><creatorcontrib>Morais, Janne K.S.</creatorcontrib><creatorcontrib>Gomes-Filho, Eneas</creatorcontrib><creatorcontrib>Vasconcelos, Ilka M.</creatorcontrib><title>Gm-TX, a new toxic protein from soybean ( Glycine max) seeds with potential for controlling insect pests</title><title>Process biochemistry (1991)</title><description>A novel toxic protein from
Glycine max seeds, named
Gm-TX, was purified by combination of differential precipitation with ammonium sulphate, DEAE-cellulose, anhydrotrypsin-sepharose 4B and Superdex 200 HR fast-protein liquid chromatography.
Gm-TX is composed of one polypeptide chain of 28
kDa (SDS-PAGE), with p
I 5.1–5.2, contains <0.5% neutral sugar, has N-terminal sequence comprised of KTISSEDSPFFNCREK, maximum absorption spectrum at 280
nm and molar extinction coefficient of 16.9. It exhibited ribonuclease activity (1821.42
±
3.34
UA/h/mgP) toward yeast RNA and promoted rabbit erythrocyte agglutination (120.4
HU/mgP) mediated by anti-
Gm-TX IgG, which was inhibited by mannose. It was devoid of trypsin inhibitory, urease and chitinase activities.
Gm-TX was lethal to mice by intraperitoneal route with LD
50 of 4.5
mg/kg. Moreover, it showed dose-dependent insecticidal activity on the major economically important pests
Callosobruchus maculatus and
Dysdercus peruvianus, producing ca. 50% and 56% mortality when incorporated into artificial diets at a level of 1.16% and 0.25%, respectively. These data indicate that
Gm-TX is a candidate protein that could be expressed in genetically transformed plants for improved resistance to insect pests.</description><subject>Biocontrol</subject><subject>Callosobruchus maculatus</subject><subject>Dysdercus peruvianus</subject><subject>Glycine max</subject><subject>Insecticidal activity</subject><subject>Plant defence</subject><subject>Plant protein</subject><subject>Soybean</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkEFLxDAQhYsouK7-BCE3FWzNJNs2PYksugqCFwVvIU0nmqVN1qSru__eLOvd08zAe294X5adAy2AQnWzLFbB69b6glHaFMAKCuwgm4Coec5ZIw7TzssmLwH4cXYS45JSDgB0kn0uhvz1_Zoo4vCHjH5jNUlpI1pHTPADiX7bonLkkiz6rbYOyaA2VyQidpH82PGTrJLajVb1xPhAtHdj8H1v3QexLqIeyQrjGE-zI6P6iGd_c5q9Pdy_zh_z55fF0_zuOdd8BmPetLwGqKquAqWwLo0BbVhNhUBNTcVoukUJrWYdY5Uy9QwaTTWKqjVYCcGn2cU-N7X4WqfPcrBRY98rh34dZT0rGz5jgiZluVfq4GMMaOQq2EGFrQQqd2DlUv6BlTuwEphMYJPvdu_DVOPbYpBRW3QaOxtSXdl5-0_CL5ZAhOg</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Oliveira, Hermogenes D.</creator><creator>Sousa, Daniele O.B.</creator><creator>Oliveira, Jose T.A.</creator><creator>Carlini, Celia R.</creator><creator>Oliveira, Henrique P.</creator><creator>Pereira, Mirella L.</creator><creator>Rocha, Raquel O.</creator><creator>Morais, Janne K.S.</creator><creator>Gomes-Filho, Eneas</creator><creator>Vasconcelos, Ilka M.</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>7T5</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20100501</creationdate><title>Gm-TX, a new toxic protein from soybean ( Glycine max) seeds with potential for controlling insect pests</title><author>Oliveira, Hermogenes D. ; Sousa, Daniele O.B. ; Oliveira, Jose T.A. ; Carlini, Celia R. ; Oliveira, Henrique P. ; Pereira, Mirella L. ; Rocha, Raquel O. ; Morais, Janne K.S. ; Gomes-Filho, Eneas ; Vasconcelos, Ilka M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-9b371166d61aae75ff1cf27088ec0f620f1c851bc2d226af7419c0ce86bfe6883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biocontrol</topic><topic>Callosobruchus maculatus</topic><topic>Dysdercus peruvianus</topic><topic>Glycine max</topic><topic>Insecticidal activity</topic><topic>Plant defence</topic><topic>Plant protein</topic><topic>Soybean</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Hermogenes D.</creatorcontrib><creatorcontrib>Sousa, Daniele O.B.</creatorcontrib><creatorcontrib>Oliveira, Jose T.A.</creatorcontrib><creatorcontrib>Carlini, Celia R.</creatorcontrib><creatorcontrib>Oliveira, Henrique P.</creatorcontrib><creatorcontrib>Pereira, Mirella L.</creatorcontrib><creatorcontrib>Rocha, Raquel O.</creatorcontrib><creatorcontrib>Morais, Janne K.S.</creatorcontrib><creatorcontrib>Gomes-Filho, Eneas</creatorcontrib><creatorcontrib>Vasconcelos, Ilka M.</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Hermogenes D.</au><au>Sousa, Daniele O.B.</au><au>Oliveira, Jose T.A.</au><au>Carlini, Celia R.</au><au>Oliveira, Henrique P.</au><au>Pereira, Mirella L.</au><au>Rocha, Raquel O.</au><au>Morais, Janne K.S.</au><au>Gomes-Filho, Eneas</au><au>Vasconcelos, Ilka M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gm-TX, a new toxic protein from soybean ( Glycine max) seeds with potential for controlling insect pests</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2010-05-01</date><risdate>2010</risdate><volume>45</volume><issue>5</issue><spage>634</spage><epage>640</epage><pages>634-640</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>A novel toxic protein from
Glycine max seeds, named
Gm-TX, was purified by combination of differential precipitation with ammonium sulphate, DEAE-cellulose, anhydrotrypsin-sepharose 4B and Superdex 200 HR fast-protein liquid chromatography.
Gm-TX is composed of one polypeptide chain of 28
kDa (SDS-PAGE), with p
I 5.1–5.2, contains <0.5% neutral sugar, has N-terminal sequence comprised of KTISSEDSPFFNCREK, maximum absorption spectrum at 280
nm and molar extinction coefficient of 16.9. It exhibited ribonuclease activity (1821.42
±
3.34
UA/h/mgP) toward yeast RNA and promoted rabbit erythrocyte agglutination (120.4
HU/mgP) mediated by anti-
Gm-TX IgG, which was inhibited by mannose. It was devoid of trypsin inhibitory, urease and chitinase activities.
Gm-TX was lethal to mice by intraperitoneal route with LD
50 of 4.5
mg/kg. Moreover, it showed dose-dependent insecticidal activity on the major economically important pests
Callosobruchus maculatus and
Dysdercus peruvianus, producing ca. 50% and 56% mortality when incorporated into artificial diets at a level of 1.16% and 0.25%, respectively. These data indicate that
Gm-TX is a candidate protein that could be expressed in genetically transformed plants for improved resistance to insect pests.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2009.12.012</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-5113 |
| ispartof | Process biochemistry (1991), 2010-05, Vol.45 (5), p.634-640 |
| issn | 1359-5113 1873-3298 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_745934280 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Biocontrol Callosobruchus maculatus Dysdercus peruvianus Glycine max Insecticidal activity Plant defence Plant protein Soybean |
| title | Gm-TX, a new toxic protein from soybean ( Glycine max) seeds with potential for controlling insect pests |
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