Genomic Organization, Expression Analysis, and Chromosomal Localization of the Mouse PEX3 Gene Encoding a Peroxisomal Assembly Protein
The peroxin Pex3p has been identified as an integral peroxisomal membrane protein in yeast where pex3 mutants lack peroxisomal remnant structures. Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested. We report here the cDNA cloning and the gen...
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| Veröffentlicht in: | Biological chemistry 2000-04, Vol.381 (4), p.337-342 |
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| creator | Muntau, Ania C. Mayerhofer, Peter U. Albet, Sabrina Schmid, Thomas E. Bugaut, Maurice Roscher, Adelbert A. Kammerer, Stefan |
| description | The peroxin Pex3p has been identified as an integral peroxisomal membrane protein in yeast where pex3 mutants lack peroxisomal remnant structures. Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested. We report here the cDNA cloning and the genomic structure of the mouse PEX3 gene. The 2 kb cDNA encodes a polypeptide of 372 amino acids (42 kDa). The gene spans a region of 30 kb, contains 12 exons and 11 introns and is located on band A of chromosome 10. The putative promoter region exhibits characteristic housekeeping features. PEX3 expression was identified in all tissues analyzed, with the strongest signals in liver and in testis, and could not be induced by fenofibrate. The data presented may be useful for the generation of a mouse model defective in PEX3 in order to clarify the yet unknown functional impact of disturbances in early peroxisomal membrane assembly. |
| doi_str_mv | 10.1515/BC.2000.044 |
| format | Article |
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Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested. We report here the cDNA cloning and the genomic structure of the mouse PEX3 gene. The 2 kb cDNA encodes a polypeptide of 372 amino acids (42 kDa). The gene spans a region of 30 kb, contains 12 exons and 11 introns and is located on band A of chromosome 10. The putative promoter region exhibits characteristic housekeeping features. PEX3 expression was identified in all tissues analyzed, with the strongest signals in liver and in testis, and could not be induced by fenofibrate. 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Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested. We report here the cDNA cloning and the genomic structure of the mouse PEX3 gene. The 2 kb cDNA encodes a polypeptide of 372 amino acids (42 kDa). The gene spans a region of 30 kb, contains 12 exons and 11 introns and is located on band A of chromosome 10. The putative promoter region exhibits characteristic housekeeping features. PEX3 expression was identified in all tissues analyzed, with the strongest signals in liver and in testis, and could not be induced by fenofibrate. The data presented may be useful for the generation of a mouse model defective in PEX3 in order to clarify the yet unknown functional impact of disturbances in early peroxisomal membrane assembly.</description><subject>Animals</subject><subject>ATP-Binding Cassette Transporters</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Exons - genetics</subject><subject>Fenofibrate - pharmacology</subject><subject>Fungal Proteins - genetics</subject><subject>Gene Expression - genetics</subject><subject>Genome</subject><subject>Introns - genetics</subject><subject>Membrane Proteins - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Peptide Biosynthesis - genetics</subject><subject>Peroxins</subject><subject>Peroxisomes - chemistry</subject><subject>Peroxisomes - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>Tissue Distribution</subject><issn>1431-6730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFP2zAUx31gGoxx4o582g6QYsd2Eh9L1sGkIorUSbtZrvMCZold7FRq9wH43BilQjtxek96v_9fT_ohdErJhAoqLq_qSU4ImRDOD9AR5YxmRcnIIfoS41M6VISzz-iQkopJXrAj9HINzvfW4LvwoJ39pwfr3QWebdcBYkw7njrd7aKNF1i7BtePwfc--l53eO6N7vYR7Fs8PAK-9ZsIeDH7w3BqBjxzxjfWPWCNFxD81o7RaYzQr7odXgQ_gHVf0adWdxFO9vMY_f45W9Y32fzu-lc9nWeGCTJk7YpWrCooiDQ5J1obWVGhuTA0NytgJc3LtuSV5GUltZBatwLy3MimBdoU7Bh9H3vXwT9vIA6qt9FA12kH6XNVciFZLguRyG8fk5RyyQlL4PkImuBjDNCqdbC9DjtFiXqToq5q9SZFJSmJPtvXblY9NP-xo5EEZCNg4wDb97sOf1UyWQp1v-RK_lgUN3VZqyV7BWZRmHY</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Muntau, Ania C.</creator><creator>Mayerhofer, Peter U.</creator><creator>Albet, Sabrina</creator><creator>Schmid, Thomas E.</creator><creator>Bugaut, Maurice</creator><creator>Roscher, Adelbert A.</creator><creator>Kammerer, Stefan</creator><general>Walter de Gruyter</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000401</creationdate><title>Genomic Organization, Expression Analysis, and Chromosomal Localization of the Mouse PEX3 Gene Encoding a Peroxisomal Assembly Protein</title><author>Muntau, Ania C. ; Mayerhofer, Peter U. ; Albet, Sabrina ; Schmid, Thomas E. ; Bugaut, Maurice ; Roscher, Adelbert A. ; Kammerer, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-fb183861e5183440aac9815a45c12cbe37127f74894789a59aaf5e22c9dfe1d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>ATP-Binding Cassette Transporters</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Exons - genetics</topic><topic>Fenofibrate - pharmacology</topic><topic>Fungal Proteins - genetics</topic><topic>Gene Expression - genetics</topic><topic>Genome</topic><topic>Introns - genetics</topic><topic>Membrane Proteins - genetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Peptide Biosynthesis - genetics</topic><topic>Peroxins</topic><topic>Peroxisomes - chemistry</topic><topic>Peroxisomes - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Sequence Analysis, DNA</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muntau, Ania C.</creatorcontrib><creatorcontrib>Mayerhofer, Peter U.</creatorcontrib><creatorcontrib>Albet, Sabrina</creatorcontrib><creatorcontrib>Schmid, Thomas E.</creatorcontrib><creatorcontrib>Bugaut, Maurice</creatorcontrib><creatorcontrib>Roscher, Adelbert A.</creatorcontrib><creatorcontrib>Kammerer, Stefan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muntau, Ania C.</au><au>Mayerhofer, Peter U.</au><au>Albet, Sabrina</au><au>Schmid, Thomas E.</au><au>Bugaut, Maurice</au><au>Roscher, Adelbert A.</au><au>Kammerer, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic Organization, Expression Analysis, and Chromosomal Localization of the Mouse PEX3 Gene Encoding a Peroxisomal Assembly Protein</atitle><jtitle>Biological chemistry</jtitle><addtitle>Biological Chemistry</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>381</volume><issue>4</issue><spage>337</spage><epage>342</epage><pages>337-342</pages><issn>1431-6730</issn><abstract>The peroxin Pex3p has been identified as an integral peroxisomal membrane protein in yeast where pex3 mutants lack peroxisomal remnant structures. Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested. We report here the cDNA cloning and the genomic structure of the mouse PEX3 gene. The 2 kb cDNA encodes a polypeptide of 372 amino acids (42 kDa). The gene spans a region of 30 kb, contains 12 exons and 11 introns and is located on band A of chromosome 10. The putative promoter region exhibits characteristic housekeeping features. PEX3 expression was identified in all tissues analyzed, with the strongest signals in liver and in testis, and could not be induced by fenofibrate. The data presented may be useful for the generation of a mouse model defective in PEX3 in order to clarify the yet unknown functional impact of disturbances in early peroxisomal membrane assembly.</abstract><cop>Germany</cop><pub>Walter de Gruyter</pub><pmid>10839463</pmid><doi>10.1515/BC.2000.044</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological chemistry, 2000-04, Vol.381 (4), p.337-342 |
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| subjects | Animals ATP-Binding Cassette Transporters Chromosome Mapping Cloning, Molecular DNA, Complementary Exons - genetics Fenofibrate - pharmacology Fungal Proteins - genetics Gene Expression - genetics Genome Introns - genetics Membrane Proteins - genetics Mice Molecular Sequence Data Peptide Biosynthesis - genetics Peroxins Peroxisomes - chemistry Peroxisomes - metabolism Polymerase Chain Reaction Promoter Regions, Genetic - genetics RNA, Messenger - drug effects RNA, Messenger - metabolism Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Tissue Distribution |
| title | Genomic Organization, Expression Analysis, and Chromosomal Localization of the Mouse PEX3 Gene Encoding a Peroxisomal Assembly Protein |
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