Immunoglobulin G from Patients with Graves’ Disease Induces Interleukin-16 and RANTES Expression in Cultured Human Thyrocytes: A Putative Mechanism for T-Cell Infiltration of the Thyroid in Autoimmune Disease

Mechanisms underlying lymphocyte infiltration of the thyroid gland and orbit in Graves’ disease (GD) are poorly understood. The IGF-I receptor (IGF-IR) is a newly recognized self-antigen that, when activated in GD fibroblasts by IGF-I or GD-IgGs, provokes the expression of IL-16 and RANTES (regulate...

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Veröffentlicht in:Endocrinology (Philadelphia) 2006-04, Vol.147 (4), p.1941-1949
Hauptverfasser: Gianoukakis, Andrew G, Douglas, Raymond S, King, Chris S, Cruikshank, William W, Smith, Terry J
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container_end_page 1949
container_issue 4
container_start_page 1941
container_title Endocrinology (Philadelphia)
container_volume 147
creator Gianoukakis, Andrew G
Douglas, Raymond S
King, Chris S
Cruikshank, William W
Smith, Terry J
description Mechanisms underlying lymphocyte infiltration of the thyroid gland and orbit in Graves’ disease (GD) are poorly understood. The IGF-I receptor (IGF-IR) is a newly recognized self-antigen that, when activated in GD fibroblasts by IGF-I or GD-IgGs, provokes the expression of IL-16 and RANTES (regulated upon activation, normal T cell expressed and secreted)-dependent T lymphocyte chemoattraction and hyaluronan synthesis. IL-16 is a CD4+-specific ligand, and RANTES is a C-C chemokine. Here we report that IGF-I and GD-IgG could induce IL-16 and RANTES in cultured human thyrocytes in a time-dependent manner. Importantly, human TSH failed to induce either chemoattractant. This induction could be attenuated by dexamethasone. Rapamycin, a specific inhibitor of the FRAP/mammalian target of rapamycin/p70s6k pathway, prevented GD-IgG-provoked IL-16 synthesis. IH7, a monoclonal antibody directed at IGF-IR also blocked the induction of chemoattraction as well as RANTES mRNA synthesis. Our findings suggest that thyrocytes can be activated by GD-IgG and IGF-I to express powerful T-cell chemoattractants. These actions of GD-IgG appear to be mediated through pathways independent of the TSH receptor. Thus, in GD, thyrocytes may participate directly in lymphocyte recruitment through their expression of IL-16 and RANTES.
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The IGF-I receptor (IGF-IR) is a newly recognized self-antigen that, when activated in GD fibroblasts by IGF-I or GD-IgGs, provokes the expression of IL-16 and RANTES (regulated upon activation, normal T cell expressed and secreted)-dependent T lymphocyte chemoattraction and hyaluronan synthesis. IL-16 is a CD4+-specific ligand, and RANTES is a C-C chemokine. Here we report that IGF-I and GD-IgG could induce IL-16 and RANTES in cultured human thyrocytes in a time-dependent manner. Importantly, human TSH failed to induce either chemoattractant. This induction could be attenuated by dexamethasone. Rapamycin, a specific inhibitor of the FRAP/mammalian target of rapamycin/p70s6k pathway, prevented GD-IgG-provoked IL-16 synthesis. IH7, a monoclonal antibody directed at IGF-IR also blocked the induction of chemoattraction as well as RANTES mRNA synthesis. Our findings suggest that thyrocytes can be activated by GD-IgG and IGF-I to express powerful T-cell chemoattractants. 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The IGF-I receptor (IGF-IR) is a newly recognized self-antigen that, when activated in GD fibroblasts by IGF-I or GD-IgGs, provokes the expression of IL-16 and RANTES (regulated upon activation, normal T cell expressed and secreted)-dependent T lymphocyte chemoattraction and hyaluronan synthesis. IL-16 is a CD4+-specific ligand, and RANTES is a C-C chemokine. Here we report that IGF-I and GD-IgG could induce IL-16 and RANTES in cultured human thyrocytes in a time-dependent manner. Importantly, human TSH failed to induce either chemoattractant. This induction could be attenuated by dexamethasone. Rapamycin, a specific inhibitor of the FRAP/mammalian target of rapamycin/p70s6k pathway, prevented GD-IgG-provoked IL-16 synthesis. IH7, a monoclonal antibody directed at IGF-IR also blocked the induction of chemoattraction as well as RANTES mRNA synthesis. Our findings suggest that thyrocytes can be activated by GD-IgG and IGF-I to express powerful T-cell chemoattractants. These actions of GD-IgG appear to be mediated through pathways independent of the TSH receptor. Thus, in GD, thyrocytes may participate directly in lymphocyte recruitment through their expression of IL-16 and RANTES.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>16410300</pmid><doi>10.1210/en.2005-1375</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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subjects Antibodies, Monoclonal - immunology
Autoantigens
Autoimmune diseases
Autoimmune Diseases - pathology
CD4 antigen
Cell activation
Cell Movement
Cells, Cultured
Chemokine CCL5 - genetics
Chemotactic factors
Dexamethasone
Dexamethasone - pharmacology
Graves disease
Graves Disease - immunology
Humans
Hyaluronic acid
IgG antibody
Immunoglobulin G
Immunoglobulin G - pharmacology
Infiltration
Insulin-like growth factor I
Insulin-like growth factor I receptors
Interleukin 16
Interleukin-16 - genetics
Lymphocytes
Lymphocytes T
Monoclonal antibodies
RANTES
Rapamycin
Receptor, IGF Type 1 - analysis
Receptors
Receptors, Thyrotropin - analysis
RNA, Messenger - analysis
Sirolimus - pharmacology
Synthesis
T-Lymphocytes - physiology
Thyrocytes
Thyroid
Thyroid gland
Thyroid Gland - metabolism
Thyroid Gland - pathology
Thyroid-stimulating hormone
TOR protein
Transcription
title Immunoglobulin G from Patients with Graves’ Disease Induces Interleukin-16 and RANTES Expression in Cultured Human Thyrocytes: A Putative Mechanism for T-Cell Infiltration of the Thyroid in Autoimmune Disease
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