High throughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurements

A dynamic respiration assay based on luminescence decay time detection of oxygen for high throughput toxicological assessment is presented. The method uses 24-well plates (OxoDishes) read with the help of a sensor dish reader placed in a humidified CO 2-incubator. Adherent primary rat hepatocytes an...

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Veröffentlicht in:	Toxicology in vitro 2010-03, Vol.24 (2), p.686-694
Hauptverfasser:	Beckers, Simone, Noor, Fozia, Müller-Vieira, Ursula, Mayer, Manuela, Strigun, Alexander, Heinzle, Elmar
Format:	Artikel
Sprache:	eng
Schlagworte:	Adherent cells Animals Biological Assay - methods Cell Adhesion Cell Line, Tumor Cytotoxicity Cytotoxins - toxicity Drug Evaluation, Preclinical - methods Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - metabolism Humans Luminescence decay time Male Oxygen - metabolism Oxygen Consumption - drug effects Oxygen Consumption - physiology Oxygen sensor Pharmacokinetics Rats Rats, Wistar Respiration Screening Time resolved oxygen sensing Toxicity Tests - methods
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