Flow Cytometric Application of Helper Adenovirus (HAd) Containing GFP Gene Flanked by Two Parallel loxP Sites to Evaluation of 293 Cre-Complementing Cell Line and Monitoring of HAd in Gutless Ad Production

Gutless adenoviruses (GAds), namely, all gene‐deleted adenoviruses, were developed to minimize their immune responses and toxic effects for a successful gene delivery tool in gene therapy. The Cre/ loxP system has been widely used for GAd production. To produce GAd with a low amount of helper adenov...

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Veröffentlicht in:	Biotechnology progress 2004-05, Vol.20 (3), p.913-920
Hauptverfasser:	Park, Min Tae, Hwang, Su-Jeong, Lee, Gyun Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics Adenoviridae - isolation & purification Adenoviridae - physiology Adenovirus Biological and medical sciences Biotechnology Cell Line Cell Separation - methods CRE protein Flow cytometry Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Gene therapy Genes, Reporter - genetics Genetic Engineering - methods Green fluorescent protein Green Fluorescent Proteins - genetics Helper viruses Humans Integrases - analysis Integrases - genetics Integrases - metabolism Kidney - virology LoxP gene Q1 Q2 Viral Proteins - analysis Viral Proteins - genetics Viral Proteins - metabolism Virus Cultivation - methods Western blotting
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