Optimization of cryoprotectants for cryopreservation of rat hepatocyte
Rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing either fetal bovine serum (FBS), glycerol, dimethyl sulfoxide (DMSO), sucrose or a mixture of these as a cryoprotectant. The best survival was with 10% (v/v) DMSO containing 30% (v/v) FBS using 5 x 10(5) hepatocytes ml(...
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| Veröffentlicht in: | Biotechnology letters 2004-05, Vol.26 (10), p.829-833 |
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| container_title | Biotechnology letters |
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| creator | JEONG HWA SON KIM, Ki-Hong NAM, Yoon-Kwon PARK, Jung-Keug KIM, Sung-Koo |
| description | Rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing either fetal bovine serum (FBS), glycerol, dimethyl sulfoxide (DMSO), sucrose or a mixture of these as a cryoprotectant. The best survival was with 10% (v/v) DMSO containing 30% (v/v) FBS using 5 x 10(5) hepatocytes ml(-1) at -70 degrees C for 5 d on type I collagen-coated dishes. After thawing, the cell viability was 81% determined by the MTT-test. The cryopreserved hepatocytes had the capacity of albumin synthesis similar to hepatocytes without cryopreservation. This result shows that cryopreservation of rat hepatocyte can be used for the evaluation of hepatic functions. |
| doi_str_mv | 10.1023/B:BILE.0000025886.57547.04 |
| format | Article |
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The best survival was with 10% (v/v) DMSO containing 30% (v/v) FBS using 5 x 10(5) hepatocytes ml(-1) at -70 degrees C for 5 d on type I collagen-coated dishes. After thawing, the cell viability was 81% determined by the MTT-test. The cryopreserved hepatocytes had the capacity of albumin synthesis similar to hepatocytes without cryopreservation. This result shows that cryopreservation of rat hepatocyte can be used for the evaluation of hepatic functions.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/B:BILE.0000025886.57547.04</identifier><identifier>PMID: 15269556</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Albumin ; Animals ; Biological and medical sciences ; Biotechnology ; Cell Culture Techniques - methods ; Cell Survival - drug effects ; Cells, Cultured ; Collagen (type I) ; Cryopreservation ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Cryoprotectors ; Dimethyl Sulfoxide - pharmacology ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Glycerol - pharmacology ; Hepatocytes ; Hepatocytes - drug effects ; Hepatocytes - physiology ; Liver ; Male ; Optimization ; Q1 ; Rats ; Rats, Sprague-Dawley ; Rodents ; Serum Albumin, Bovine - pharmacology ; Thawing</subject><ispartof>Biotechnology letters, 2004-05, Vol.26 (10), p.829-833</ispartof><rights>2004 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-1b294d998a73d1a3a00dd49ce331ed730892ae6bb3b025532dc946a14d7afa53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15730823$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15269556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JEONG HWA SON</creatorcontrib><creatorcontrib>KIM, Ki-Hong</creatorcontrib><creatorcontrib>NAM, Yoon-Kwon</creatorcontrib><creatorcontrib>PARK, Jung-Keug</creatorcontrib><creatorcontrib>KIM, Sung-Koo</creatorcontrib><title>Optimization of cryoprotectants for cryopreservation of rat hepatocyte</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing either fetal bovine serum (FBS), glycerol, dimethyl sulfoxide (DMSO), sucrose or a mixture of these as a cryoprotectant. The best survival was with 10% (v/v) DMSO containing 30% (v/v) FBS using 5 x 10(5) hepatocytes ml(-1) at -70 degrees C for 5 d on type I collagen-coated dishes. After thawing, the cell viability was 81% determined by the MTT-test. The cryopreserved hepatocytes had the capacity of albumin synthesis similar to hepatocytes without cryopreservation. This result shows that cryopreservation of rat hepatocyte can be used for the evaluation of hepatic functions.</description><subject>Albumin</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Collagen (type I)</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Cryoprotectors</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerol - pharmacology</subject><subject>Hepatocytes</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - physiology</subject><subject>Liver</subject><subject>Male</subject><subject>Optimization</subject><subject>Q1</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Serum Albumin, Bovine - pharmacology</subject><subject>Thawing</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90c9LwzAUB_AgipvTf0GGoJ468zuNNydTBwMv3sNrmmJla2aSCfOvt3XFiQdzCYRPkvfeF6ELgicEU3YzvZ3OF7MJ7hYVeS4nQgmuJpgfoCERimVSKXmIhphwkgmu6QCdxPjWcq2wOkYDIqjUQsghenhep3pVf0KqfTP21diGrV8Hn5xN0KQ4rnzoz1x04ePHBUjjV7eG5O02uVN0VMEyurN-H6GXh9nL_VO2eH6c398tMst1njJSUM1LrXNQrCTAAOOy5No6xogrFcO5puBkUbCi7UwwWlrNJRBeKqhAsBG63j3bVvi-cTGZVR2tWy6hcX4TjeJCY8oZb-XVv1JKxajkpIUXf-Cb34SmbcIopnBO8nagI3S7Qzb4GIOrzDrUKwhbQ7DpMjFT02Vi9pmY70wM7ko573_YFCtX7q_2IbTgsgcQLSyrAI2t4y_XDYYy9gW5cJUg</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>JEONG HWA SON</creator><creator>KIM, Ki-Hong</creator><creator>NAM, Yoon-Kwon</creator><creator>PARK, Jung-Keug</creator><creator>KIM, Sung-Koo</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Optimization of cryoprotectants for cryopreservation of rat hepatocyte</title><author>JEONG HWA SON ; KIM, Ki-Hong ; NAM, Yoon-Kwon ; PARK, Jung-Keug ; KIM, Sung-Koo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-1b294d998a73d1a3a00dd49ce331ed730892ae6bb3b025532dc946a14d7afa53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Albumin</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Collagen (type I)</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Cryoprotectors</topic><topic>Dimethyl Sulfoxide - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. 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| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2004-05, Vol.26 (10), p.829-833 |
| issn | 0141-5492 1573-6776 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Albumin Animals Biological and medical sciences Biotechnology Cell Culture Techniques - methods Cell Survival - drug effects Cells, Cultured Collagen (type I) Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Cryoprotectors Dimethyl Sulfoxide - pharmacology Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Glycerol - pharmacology Hepatocytes Hepatocytes - drug effects Hepatocytes - physiology Liver Male Optimization Q1 Rats Rats, Sprague-Dawley Rodents Serum Albumin, Bovine - pharmacology Thawing |
| title | Optimization of cryoprotectants for cryopreservation of rat hepatocyte |
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