Repair of Canine Mandibular Bone Defects with Bone Marrow Stromal Cells and Coral
Tissue engineering has become a new approach for repairing bone defects. Previous studies indicated that coral scaffolds had been utilized with bone marrow stromal cells (BMSCs) in a variety of approaches for bony reconstruction. In these applications, the degradation rate of the material did not ma...
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| creator | Yuan, Jie Zhang, Wen Jie Liu, Guangpeng Wei, Min Qi, Zuo Liang Liu, Wei Cui, Lei Cao, Yi Lin |
| description | Tissue engineering has become a new approach for repairing bone defects. Previous studies indicated that coral scaffolds had been utilized with bone marrow stromal cells (BMSCs) in a variety of approaches for bony reconstruction. In these applications, the degradation rate of the material did not match the rate at which bone was regenerated. In this study, a previously established 30 mm long mandibular segmental defect was repaired with engineered bone using green fluorescent protein–labeled osteogenic BMSCs seeded on porous coral (
n
= 12). Defects treated with coral alone (
n
= 12) were used as an experimental control. In the BMSCs/coral group, new bone formation was observed from 4 weeks postoperation, and bony-union was achieved after 32 postoperative weeks. The residual coral volume of the BMSCs/coral grafts at 12 weeks (20–30%) was significantly higher than that at 32 weeks (10–15%,
p
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| doi_str_mv | 10.1089/ten.tea.2009.0472 |
| format | Article |
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n
= 12). Defects treated with coral alone (
n
= 12) were used as an experimental control. In the BMSCs/coral group, new bone formation was observed from 4 weeks postoperation, and bony-union was achieved after 32 postoperative weeks. The residual coral volume of the BMSCs/coral grafts at 12 weeks (20–30%) was significantly higher than that at 32 weeks (10–15%,
p
< 0.05), which was detected by microcomputed tomography and histological examination. The engineered bone with BMSCs/coral achieved satisfactory biomechanical properties at 32 weeks postoperation, which was very close to that of the contralateral edentulous mandible. More importantly, immunostaining demonstrated that the implanted BMSCs differentiated into osteoblast-like cells. In contrast, minimal bone formation with almost solely fibrous connection was observed in the group treated with coral alone. Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and biodegradable coral can successfully repair the critical-sized segmental mandibular defects in canines and the seeding cells could be used for bony restoration.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2009.0472</identifier><identifier>PMID: 19925049</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Absorbable Implants ; Animals ; Anthozoa ; Biomechanical Phenomena ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; Bone Marrow Transplantation ; Bone regeneration ; Bone Substitutes ; Cell Differentiation ; Cells ; Dogs ; Green Fluorescent Proteins - metabolism ; Mandible ; Mandibular Injuries - diagnostic imaging ; Mandibular Injuries - pathology ; Mandibular Injuries - physiopathology ; Mandibular Injuries - surgery ; Original Articles ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteogenesis ; Physiological aspects ; Recombinant Proteins - metabolism ; Stromal Cells - metabolism ; Stromal Cells - transplantation ; Tissue engineering ; Tissue Engineering - methods ; Transplants & implants ; X-Ray Microtomography</subject><ispartof>Tissue engineering. Part A, 2010-04, Vol.16 (4), p.1385-1394</ispartof><rights>2010, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2010, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-d4f2cb140b92aa7cc0078f03f7fe3cd88ed723595303333fe64c68c939c339ae3</citedby><cites>FETCH-LOGICAL-c474t-d4f2cb140b92aa7cc0078f03f7fe3cd88ed723595303333fe64c68c939c339ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.liebertpub.com/doi/epdf/10.1089/ten.tea.2009.0472$$EPDF$$P50$$Gmaryannliebert$$H</linktopdf><linktohtml>$$Uhttps://www.liebertpub.com/doi/full/10.1089/ten.tea.2009.0472$$EHTML$$P50$$Gmaryannliebert$$H</linktohtml><link.rule.ids>314,777,781,3029,21704,27905,27906,55272,55284</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19925049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Jie</creatorcontrib><creatorcontrib>Zhang, Wen Jie</creatorcontrib><creatorcontrib>Liu, Guangpeng</creatorcontrib><creatorcontrib>Wei, Min</creatorcontrib><creatorcontrib>Qi, Zuo Liang</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Cui, Lei</creatorcontrib><creatorcontrib>Cao, Yi Lin</creatorcontrib><title>Repair of Canine Mandibular Bone Defects with Bone Marrow Stromal Cells and Coral</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Tissue engineering has become a new approach for repairing bone defects. Previous studies indicated that coral scaffolds had been utilized with bone marrow stromal cells (BMSCs) in a variety of approaches for bony reconstruction. In these applications, the degradation rate of the material did not match the rate at which bone was regenerated. In this study, a previously established 30 mm long mandibular segmental defect was repaired with engineered bone using green fluorescent protein–labeled osteogenic BMSCs seeded on porous coral (
n
= 12). Defects treated with coral alone (
n
= 12) were used as an experimental control. In the BMSCs/coral group, new bone formation was observed from 4 weeks postoperation, and bony-union was achieved after 32 postoperative weeks. The residual coral volume of the BMSCs/coral grafts at 12 weeks (20–30%) was significantly higher than that at 32 weeks (10–15%,
p
< 0.05), which was detected by microcomputed tomography and histological examination. The engineered bone with BMSCs/coral achieved satisfactory biomechanical properties at 32 weeks postoperation, which was very close to that of the contralateral edentulous mandible. More importantly, immunostaining demonstrated that the implanted BMSCs differentiated into osteoblast-like cells. In contrast, minimal bone formation with almost solely fibrous connection was observed in the group treated with coral alone. Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and biodegradable coral can successfully repair the critical-sized segmental mandibular defects in canines and the seeding cells could be used for bony restoration.</description><subject>Absorbable Implants</subject><subject>Animals</subject><subject>Anthozoa</subject><subject>Biomechanical Phenomena</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Marrow Transplantation</subject><subject>Bone regeneration</subject><subject>Bone Substitutes</subject><subject>Cell Differentiation</subject><subject>Cells</subject><subject>Dogs</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Mandible</subject><subject>Mandibular Injuries - diagnostic imaging</subject><subject>Mandibular Injuries - pathology</subject><subject>Mandibular Injuries - physiopathology</subject><subject>Mandibular Injuries - surgery</subject><subject>Original Articles</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis</subject><subject>Physiological aspects</subject><subject>Recombinant Proteins - metabolism</subject><subject>Stromal Cells - metabolism</subject><subject>Stromal Cells - transplantation</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Transplants & implants</subject><subject>X-Ray Microtomography</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkV2L1TAQhoMo7rr6A7yRoBdenTpp0pPmcq2fsIv4Bd6FNJ1olrY5m6Qs_ntTelAUQRPCJMPzDpN5CXnIoGLQqmcZ5yqjqWoAVYGQ9S1yyhSXO86bL7d_3gU7IfdSugLYw17Ku-SEKVU3INQpef8BD8ZHGhztzOxnpJdmHny_jCbS56G8X6BDmxO98fnblrk0MYYb-jHHMJmRdjiOiRYV7UI0431yx5kx4YNjPCOfX7381L3ZXbx7_bY7v9hZIUXeDcLVtmcCelUbI60FkK0D7qRDboe2xUHWvFENB16Ww72w-9YqriznyiA_I0-3uocYrhdMWU8-2dKLmTEsSUvRKGC8bf5NlnFJqRpZyMd_kFdhiXP5hq6BSQ7A2wI92aCvZkTtZxdyNHYtqc9r0Qook-WFqv5ClT3g5G0Zo_Ml_5uAbQIbQ0oRnT5EP5n4XTPQq9262F2O0avderW7aB4d-136CYdfiqO_BZAbsKbNPI8ee4z5P0r_APfotmw</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Yuan, Jie</creator><creator>Zhang, Wen Jie</creator><creator>Liu, Guangpeng</creator><creator>Wei, Min</creator><creator>Qi, Zuo Liang</creator><creator>Liu, Wei</creator><creator>Cui, Lei</creator><creator>Cao, Yi Lin</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100401</creationdate><title>Repair of Canine Mandibular Bone Defects with Bone Marrow Stromal Cells and Coral</title><author>Yuan, Jie ; Zhang, Wen Jie ; Liu, Guangpeng ; Wei, Min ; Qi, Zuo Liang ; Liu, Wei ; Cui, Lei ; Cao, Yi Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-d4f2cb140b92aa7cc0078f03f7fe3cd88ed723595303333fe64c68c939c339ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Absorbable Implants</topic><topic>Animals</topic><topic>Anthozoa</topic><topic>Biomechanical Phenomena</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Marrow Transplantation</topic><topic>Bone regeneration</topic><topic>Bone Substitutes</topic><topic>Cell Differentiation</topic><topic>Cells</topic><topic>Dogs</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Mandible</topic><topic>Mandibular Injuries - diagnostic imaging</topic><topic>Mandibular Injuries - pathology</topic><topic>Mandibular Injuries - physiopathology</topic><topic>Mandibular Injuries - surgery</topic><topic>Original Articles</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Osteogenesis</topic><topic>Physiological aspects</topic><topic>Recombinant Proteins - metabolism</topic><topic>Stromal Cells - metabolism</topic><topic>Stromal Cells - transplantation</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Transplants & implants</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Jie</creatorcontrib><creatorcontrib>Zhang, Wen Jie</creatorcontrib><creatorcontrib>Liu, Guangpeng</creatorcontrib><creatorcontrib>Wei, Min</creatorcontrib><creatorcontrib>Qi, Zuo Liang</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Cui, Lei</creatorcontrib><creatorcontrib>Cao, Yi Lin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Jie</au><au>Zhang, Wen Jie</au><au>Liu, Guangpeng</au><au>Wei, Min</au><au>Qi, Zuo Liang</au><au>Liu, Wei</au><au>Cui, Lei</au><au>Cao, Yi Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repair of Canine Mandibular Bone Defects with Bone Marrow Stromal Cells and Coral</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>16</volume><issue>4</issue><spage>1385</spage><epage>1394</epage><pages>1385-1394</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Tissue engineering has become a new approach for repairing bone defects. Previous studies indicated that coral scaffolds had been utilized with bone marrow stromal cells (BMSCs) in a variety of approaches for bony reconstruction. In these applications, the degradation rate of the material did not match the rate at which bone was regenerated. In this study, a previously established 30 mm long mandibular segmental defect was repaired with engineered bone using green fluorescent protein–labeled osteogenic BMSCs seeded on porous coral (
n
= 12). Defects treated with coral alone (
n
= 12) were used as an experimental control. In the BMSCs/coral group, new bone formation was observed from 4 weeks postoperation, and bony-union was achieved after 32 postoperative weeks. The residual coral volume of the BMSCs/coral grafts at 12 weeks (20–30%) was significantly higher than that at 32 weeks (10–15%,
p
< 0.05), which was detected by microcomputed tomography and histological examination. The engineered bone with BMSCs/coral achieved satisfactory biomechanical properties at 32 weeks postoperation, which was very close to that of the contralateral edentulous mandible. More importantly, immunostaining demonstrated that the implanted BMSCs differentiated into osteoblast-like cells. In contrast, minimal bone formation with almost solely fibrous connection was observed in the group treated with coral alone. Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and biodegradable coral can successfully repair the critical-sized segmental mandibular defects in canines and the seeding cells could be used for bony restoration.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>19925049</pmid><doi>10.1089/ten.tea.2009.0472</doi><tpages>10</tpages></addata></record> |
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| ispartof | Tissue engineering. Part A, 2010-04, Vol.16 (4), p.1385-1394 |
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| subjects | Absorbable Implants Animals Anthozoa Biomechanical Phenomena Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Bone Marrow Transplantation Bone regeneration Bone Substitutes Cell Differentiation Cells Dogs Green Fluorescent Proteins - metabolism Mandible Mandibular Injuries - diagnostic imaging Mandibular Injuries - pathology Mandibular Injuries - physiopathology Mandibular Injuries - surgery Original Articles Osteoblasts - cytology Osteoblasts - metabolism Osteogenesis Physiological aspects Recombinant Proteins - metabolism Stromal Cells - metabolism Stromal Cells - transplantation Tissue engineering Tissue Engineering - methods Transplants & implants X-Ray Microtomography |
| title | Repair of Canine Mandibular Bone Defects with Bone Marrow Stromal Cells and Coral |
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