Preparation and some properties of human serum kallikrein immobilized on arm-sepharose

A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. K M values for N-benzoyl- l-arginine ethyl ester and N-tosyl- l-arginine methyl ester hydrolysis of this preparation were 1.10 × 10≈ 3 M and 3.6 × 10 −4 M, respectively; pH optimum of hydrolysis of these esters we...

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Veröffentlicht in:	Clinica chimica acta 1979-05, Vol.93 (3), p.329-333
Hauptverfasser:	Kawalec, M., Kawiak, J., Yarovaya, G.A., Dotsenko, V.L., Oorekhovich, V.N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Arginine - analogs & derivatives Arginine - metabolism Enzymes, Immobilized Humans Hydrogen-Ion Concentration Kallikreins - isolation & purification Kallikreins - metabolism Tosylarginine Methyl Ester - metabolism
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container_title	Clinica chimica acta
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creator	Kawalec, M. Kawiak, J. Yarovaya, G.A. Dotsenko, V.L. Oorekhovich, V.N.
description	A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. K M values for N-benzoyl- l-arginine ethyl ester and N-tosyl- l-arginine methyl ester hydrolysis of this preparation were 1.10 × 10≈ 3 M and 3.6 × 10 −4 M, respectively; pH optimum of hydrolysis of these esters were found to be 8.2 and 8.5, respectively. The immobilized kallikrein possessed kininogenase activity and was capable of activating prekallikrein.
doi_str_mv	10.1016/0009-8981(79)90282-1
format	Article
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K M values for N-benzoyl- l-arginine ethyl ester and N-tosyl- l-arginine methyl ester hydrolysis of this preparation were 1.10 × 10≈ 3 M and 3.6 × 10 −4 M, respectively; pH optimum of hydrolysis of these esters were found to be 8.2 and 8.5, respectively. 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The immobilized kallikrein possessed kininogenase activity and was capable of activating prekallikrein.</description><subject>Arginine - analogs & derivatives</subject><subject>Arginine - metabolism</subject><subject>Enzymes, Immobilized</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kallikreins - isolation & purification</subject><subject>Kallikreins - metabolism</subject><subject>Tosylarginine Methyl Ester - metabolism</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYM46Pj4BbrISnRRTZo-ko0ggy8Y0IW6DWlyi9GmrUkr6K83nRlm6epyuecczv0QOqXkkhJaXBFCRMIFp-eluBAk5WlCd9Cc8pIlLBPpLppvJfvoIISPuGakoHtoxoo0K-bo7dlDr7wabNdi1RocOge4910PfrAQcFfj99GpFgfwo8OfqmnspwfbYutcV9nG_oLBk9m7JED_rnwX4AjNatUEON7MQ_R6d_uyeEiWT_ePi5tlolmeD7FYyhgri9ooI4Bokhuqda1zkhpaF6nONdAqA8Z1XdPKCJHzqorWIuOCMcIO0dk6Nzb-GiEM0tmgoWlUC90YZJnlnNOMRmG2FupYL3ioZe-tU_5HUiInmHIiJSdSshRyBVNOtpNN_lg5MFvTil68Xq-vEF_8tuBl0BZaDcZ60IM0nf0__g9N7YR6</recordid><startdate>19790502</startdate><enddate>19790502</enddate><creator>Kawalec, M.</creator><creator>Kawiak, J.</creator><creator>Yarovaya, G.A.</creator><creator>Dotsenko, V.L.</creator><creator>Oorekhovich, V.N.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19790502</creationdate><title>Preparation and some properties of human serum kallikrein immobilized on arm-sepharose</title><author>Kawalec, M. ; Kawiak, J. ; Yarovaya, G.A. ; Dotsenko, V.L. ; Oorekhovich, V.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-89233376fdad9e0c05d1ccfc502d1f62c5ce1b4e38cff1bd9958bb35564893303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Arginine - analogs & derivatives</topic><topic>Arginine - metabolism</topic><topic>Enzymes, Immobilized</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kallikreins - isolation & purification</topic><topic>Kallikreins - metabolism</topic><topic>Tosylarginine Methyl Ester - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawalec, M.</creatorcontrib><creatorcontrib>Kawiak, J.</creatorcontrib><creatorcontrib>Yarovaya, G.A.</creatorcontrib><creatorcontrib>Dotsenko, V.L.</creatorcontrib><creatorcontrib>Oorekhovich, V.N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawalec, M.</au><au>Kawiak, J.</au><au>Yarovaya, G.A.</au><au>Dotsenko, V.L.</au><au>Oorekhovich, V.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and some properties of human serum kallikrein immobilized on arm-sepharose</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1979-05-02</date><risdate>1979</risdate><volume>93</volume><issue>3</issue><spage>329</spage><epage>333</epage><pages>329-333</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. K M values for N-benzoyl- l-arginine ethyl ester and N-tosyl- l-arginine methyl ester hydrolysis of this preparation were 1.10 × 10≈ 3 M and 3.6 × 10 −4 M, respectively; pH optimum of hydrolysis of these esters were found to be 8.2 and 8.5, respectively. The immobilized kallikrein possessed kininogenase activity and was capable of activating prekallikrein.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36246</pmid><doi>10.1016/0009-8981(79)90282-1</doi><tpages>5</tpages></addata></record>
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source	MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects	Arginine - analogs & derivatives Arginine - metabolism Enzymes, Immobilized Humans Hydrogen-Ion Concentration Kallikreins - isolation & purification Kallikreins - metabolism Tosylarginine Methyl Ester - metabolism
title	Preparation and some properties of human serum kallikrein immobilized on arm-sepharose
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