Cell line misidentification: the beginning of the end
That a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species has been a long known, but largely ignored problem. The history of cell line misidentification and recent efforts to develop a standard for the authentication of hum...
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| Veröffentlicht in: | Nature reviews. Cancer 2010-06, Vol.10 (6), p.441-448 |
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| container_title | Nature reviews. Cancer |
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| creator | Masters, John R W |
| description | That a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species has been a long known, but largely ignored problem. The history of cell line misidentification and recent efforts to develop a standard for the authentication of human cell lines using short tandem repeat profiling is discussed in this article.
Cell lines are used extensively in research and drug development as models of normal and cancer tissues. However, a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species. The scientific community has failed to tackle this problem and consequently thousands of misleading and potentially erroneous papers have been published using cell lines that are incorrectly identified. Recent efforts to develop a standard for the authentication of human cell lines using short tandem repeat profiling is an important step to eradicate this problem. |
| doi_str_mv | 10.1038/nrc2852 |
| format | Article |
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Cell lines are used extensively in research and drug development as models of normal and cancer tissues. However, a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species. The scientific community has failed to tackle this problem and consequently thousands of misleading and potentially erroneous papers have been published using cell lines that are incorrectly identified. Recent efforts to develop a standard for the authentication of human cell lines using short tandem repeat profiling is an important step to eradicate this problem.</description><identifier>ISSN: 1474-175X</identifier><identifier>EISSN: 1474-1768</identifier><identifier>DOI: 10.1038/nrc2852</identifier><identifier>PMID: 20448633</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/1513 ; 631/67/70 ; Affinity labeling ; Animals ; Bibliometrics ; Biomedical and Life Sciences ; Biomedicine ; Cancer ; Cancer cells ; Cancer Research ; Cell Line ; Cell lines ; Databases, Genetic ; Gene Expression Profiling - standards ; Humans ; Identification and classification ; Methods ; Microsatellite Repeats ; Oncology, Experimental ; Peer Review, Research ; science-and-society</subject><ispartof>Nature reviews. Cancer, 2010-06, Vol.10 (6), p.441-448</ispartof><rights>Springer Nature Limited 2010</rights><rights>COPYRIGHT 2010 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-81a7b18841b14c9b2f4af087624cbb44014cdef5b6bec9952cf27fcffc31925b3</citedby><cites>FETCH-LOGICAL-c470t-81a7b18841b14c9b2f4af087624cbb44014cdef5b6bec9952cf27fcffc31925b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nrc2852$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nrc2852$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>313,314,776,780,788,27899,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20448633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masters, John R W</creatorcontrib><creatorcontrib>American Type Culture Collection Standards Development Organization Workgroup ASN-0002</creatorcontrib><title>Cell line misidentification: the beginning of the end</title><title>Nature reviews. Cancer</title><addtitle>Nat Rev Cancer</addtitle><addtitle>Nat Rev Cancer</addtitle><description>That a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species has been a long known, but largely ignored problem. The history of cell line misidentification and recent efforts to develop a standard for the authentication of human cell lines using short tandem repeat profiling is discussed in this article.
Cell lines are used extensively in research and drug development as models of normal and cancer tissues. However, a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species. The scientific community has failed to tackle this problem and consequently thousands of misleading and potentially erroneous papers have been published using cell lines that are incorrectly identified. 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Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masters, John R W</au><aucorp>American Type Culture Collection Standards Development Organization Workgroup ASN-0002</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell line misidentification: the beginning of the end</atitle><jtitle>Nature reviews. Cancer</jtitle><stitle>Nat Rev Cancer</stitle><addtitle>Nat Rev Cancer</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>10</volume><issue>6</issue><spage>441</spage><epage>448</epage><pages>441-448</pages><issn>1474-175X</issn><eissn>1474-1768</eissn><abstract>That a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species has been a long known, but largely ignored problem. The history of cell line misidentification and recent efforts to develop a standard for the authentication of human cell lines using short tandem repeat profiling is discussed in this article.
Cell lines are used extensively in research and drug development as models of normal and cancer tissues. However, a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species. The scientific community has failed to tackle this problem and consequently thousands of misleading and potentially erroneous papers have been published using cell lines that are incorrectly identified. Recent efforts to develop a standard for the authentication of human cell lines using short tandem repeat profiling is an important step to eradicate this problem.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>20448633</pmid><doi>10.1038/nrc2852</doi><tpages>8</tpages></addata></record> |
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| subjects | 631/1647/1513 631/67/70 Affinity labeling Animals Bibliometrics Biomedical and Life Sciences Biomedicine Cancer Cancer cells Cancer Research Cell Line Cell lines Databases, Genetic Gene Expression Profiling - standards Humans Identification and classification Methods Microsatellite Repeats Oncology, Experimental Peer Review, Research science-and-society |
| title | Cell line misidentification: the beginning of the end |
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