Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy
In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between -634C/G, -460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum level...
Gespeichert in:
| Veröffentlicht in: | American journal of reproductive immunology (1989) 2010-02, Vol.63 (2), p.120-125 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 125 |
|---|---|
| container_issue | 2 |
| container_start_page | 120 |
| container_title | American journal of reproductive immunology (1989) |
| container_volume | 63 |
| creator | Elito, Jr, Julio Daher, Silvia Fernandes da Silva, Marcelo Octávio Marconi, Natali Maiumi Higashi Pendeloski, Karen Priscilla Tezotto Moron, Antonio Fernandes Camano, Luiz |
| description | In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between -634C/G, -460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.
of study This is a case-control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post-menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. For 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (-634C/G, -460T/C, and +936C/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.
The -634C/G, -460T/C, and +936C/T VEGF polymorphisms were not associated with ectopic pregnancy (P = 0.170, P = 0.285, and P = 0.700, respectively). The serum levels of VEGF were not associated with the genotype of -634C/G, -460T/C, and +936C/T VEGF polymorphisms (P = 0.702; P = 0.347, and P = 0.256, respectively).
There was no association between ectopic pregnancy and -634C/G, -460T/C, and +936C/T VEGF polymorphisms. There was no correlation between VEGF genotype and the expression of VEGF in blood samples. |
| doi_str_mv | 10.1111/j.1600-0897.2009.00769.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_745711309</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>745711309</sourcerecordid><originalsourceid>FETCH-LOGICAL-p242t-88c0a48e23f5901fb67293886269040cd537297e472c51634eba978c1070a3243</originalsourceid><addsrcrecordid>eNqFkEtPwzAQhC0EolD4C8g3TgnrR2L7WFW8pEpc4By5jtO6SuJgJ5T-e4woXNnLrGa_mcMihAnkJM3dLiclQAZSiZwCqBxAlCr_PEEXf4fTtAMvM8FBztBljDuA5DNxjmYpQwpG1QVqFzF64_TofI_jONUH7Bv8oaOZWh2w7Ws_bm3rdIs3we_HLW60GX3Auq_x4NtD58OwdbGL3zk3RryxvcV7l0ibwMEZPAS76XVvDlforNFttNdHnaO3h_vX5VO2enl8Xi5W2UA5HTMpDWguLWVNoYA061JQxaQsaamAg6kLlgxhuaCmICXjdq2VkIaAAM0oZ3N0-9M7BP8-2ThWnYvGtq3urZ9iJXghCGGg_icZKwtJlEjkzZGc1p2tqyG4TodD9ftK9gXuCXe8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733658197</pqid></control><display><type>article</type><title>Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Elito, Jr, Julio ; Daher, Silvia ; Fernandes da Silva, Marcelo Octávio ; Marconi, Natali Maiumi Higashi ; Pendeloski, Karen Priscilla Tezotto ; Moron, Antonio Fernandes ; Camano, Luiz</creator><creatorcontrib>Elito, Jr, Julio ; Daher, Silvia ; Fernandes da Silva, Marcelo Octávio ; Marconi, Natali Maiumi Higashi ; Pendeloski, Karen Priscilla Tezotto ; Moron, Antonio Fernandes ; Camano, Luiz</creatorcontrib><description>In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between -634C/G, -460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.
of study This is a case-control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post-menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. For 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (-634C/G, -460T/C, and +936C/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.
The -634C/G, -460T/C, and +936C/T VEGF polymorphisms were not associated with ectopic pregnancy (P = 0.170, P = 0.285, and P = 0.700, respectively). The serum levels of VEGF were not associated with the genotype of -634C/G, -460T/C, and +936C/T VEGF polymorphisms (P = 0.702; P = 0.347, and P = 0.256, respectively).
There was no association between ectopic pregnancy and -634C/G, -460T/C, and +936C/T VEGF polymorphisms. There was no correlation between VEGF genotype and the expression of VEGF in blood samples.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2009.00769.x</identifier><identifier>PMID: 20015329</identifier><language>eng</language><publisher>Denmark</publisher><subject>Adult ; Alleles ; Base Sequence ; Brazil ; Case-Control Studies ; DNA Primers - genetics ; Female ; Gene Frequency ; Genotype ; Haplotypes ; Humans ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Pregnancy ; Pregnancy, Ectopic - blood ; Pregnancy, Ectopic - genetics ; Vascular Endothelial Growth Factor A - blood ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>American journal of reproductive immunology (1989), 2010-02, Vol.63 (2), p.120-125</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20015329$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elito, Jr, Julio</creatorcontrib><creatorcontrib>Daher, Silvia</creatorcontrib><creatorcontrib>Fernandes da Silva, Marcelo Octávio</creatorcontrib><creatorcontrib>Marconi, Natali Maiumi Higashi</creatorcontrib><creatorcontrib>Pendeloski, Karen Priscilla Tezotto</creatorcontrib><creatorcontrib>Moron, Antonio Fernandes</creatorcontrib><creatorcontrib>Camano, Luiz</creatorcontrib><title>Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between -634C/G, -460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.
of study This is a case-control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post-menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. For 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (-634C/G, -460T/C, and +936C/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.
The -634C/G, -460T/C, and +936C/T VEGF polymorphisms were not associated with ectopic pregnancy (P = 0.170, P = 0.285, and P = 0.700, respectively). The serum levels of VEGF were not associated with the genotype of -634C/G, -460T/C, and +936C/T VEGF polymorphisms (P = 0.702; P = 0.347, and P = 0.256, respectively).
There was no association between ectopic pregnancy and -634C/G, -460T/C, and +936C/T VEGF polymorphisms. There was no correlation between VEGF genotype and the expression of VEGF in blood samples.</description><subject>Adult</subject><subject>Alleles</subject><subject>Base Sequence</subject><subject>Brazil</subject><subject>Case-Control Studies</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Gene Frequency</subject><subject>Genotype</subject><subject>Haplotypes</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Pregnancy</subject><subject>Pregnancy, Ectopic - blood</subject><subject>Pregnancy, Ectopic - genetics</subject><subject>Vascular Endothelial Growth Factor A - blood</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPwzAQhC0EolD4C8g3TgnrR2L7WFW8pEpc4By5jtO6SuJgJ5T-e4woXNnLrGa_mcMihAnkJM3dLiclQAZSiZwCqBxAlCr_PEEXf4fTtAMvM8FBztBljDuA5DNxjmYpQwpG1QVqFzF64_TofI_jONUH7Bv8oaOZWh2w7Ws_bm3rdIs3we_HLW60GX3Auq_x4NtD58OwdbGL3zk3RryxvcV7l0ibwMEZPAS76XVvDlforNFttNdHnaO3h_vX5VO2enl8Xi5W2UA5HTMpDWguLWVNoYA061JQxaQsaamAg6kLlgxhuaCmICXjdq2VkIaAAM0oZ3N0-9M7BP8-2ThWnYvGtq3urZ9iJXghCGGg_icZKwtJlEjkzZGc1p2tqyG4TodD9ftK9gXuCXe8</recordid><startdate>201002</startdate><enddate>201002</enddate><creator>Elito, Jr, Julio</creator><creator>Daher, Silvia</creator><creator>Fernandes da Silva, Marcelo Octávio</creator><creator>Marconi, Natali Maiumi Higashi</creator><creator>Pendeloski, Karen Priscilla Tezotto</creator><creator>Moron, Antonio Fernandes</creator><creator>Camano, Luiz</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201002</creationdate><title>Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy</title><author>Elito, Jr, Julio ; Daher, Silvia ; Fernandes da Silva, Marcelo Octávio ; Marconi, Natali Maiumi Higashi ; Pendeloski, Karen Priscilla Tezotto ; Moron, Antonio Fernandes ; Camano, Luiz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p242t-88c0a48e23f5901fb67293886269040cd537297e472c51634eba978c1070a3243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Alleles</topic><topic>Base Sequence</topic><topic>Brazil</topic><topic>Case-Control Studies</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Gene Frequency</topic><topic>Genotype</topic><topic>Haplotypes</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Pregnancy</topic><topic>Pregnancy, Ectopic - blood</topic><topic>Pregnancy, Ectopic - genetics</topic><topic>Vascular Endothelial Growth Factor A - blood</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elito, Jr, Julio</creatorcontrib><creatorcontrib>Daher, Silvia</creatorcontrib><creatorcontrib>Fernandes da Silva, Marcelo Octávio</creatorcontrib><creatorcontrib>Marconi, Natali Maiumi Higashi</creatorcontrib><creatorcontrib>Pendeloski, Karen Priscilla Tezotto</creatorcontrib><creatorcontrib>Moron, Antonio Fernandes</creatorcontrib><creatorcontrib>Camano, Luiz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elito, Jr, Julio</au><au>Daher, Silvia</au><au>Fernandes da Silva, Marcelo Octávio</au><au>Marconi, Natali Maiumi Higashi</au><au>Pendeloski, Karen Priscilla Tezotto</au><au>Moron, Antonio Fernandes</au><au>Camano, Luiz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2010-02</date><risdate>2010</risdate><volume>63</volume><issue>2</issue><spage>120</spage><epage>125</epage><pages>120-125</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between -634C/G, -460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.
of study This is a case-control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post-menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. For 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (-634C/G, -460T/C, and +936C/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.
The -634C/G, -460T/C, and +936C/T VEGF polymorphisms were not associated with ectopic pregnancy (P = 0.170, P = 0.285, and P = 0.700, respectively). The serum levels of VEGF were not associated with the genotype of -634C/G, -460T/C, and +936C/T VEGF polymorphisms (P = 0.702; P = 0.347, and P = 0.256, respectively).
There was no association between ectopic pregnancy and -634C/G, -460T/C, and +936C/T VEGF polymorphisms. There was no correlation between VEGF genotype and the expression of VEGF in blood samples.</abstract><cop>Denmark</cop><pmid>20015329</pmid><doi>10.1111/j.1600-0897.2009.00769.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2010-02, Vol.63 (2), p.120-125 |
| issn | 1046-7408 1600-0897 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_745711309 |
| source | MEDLINE; Wiley Journals |
| subjects | Adult Alleles Base Sequence Brazil Case-Control Studies DNA Primers - genetics Female Gene Frequency Genotype Haplotypes Humans Middle Aged Polymerase Chain Reaction Polymorphism, Single Nucleotide Pregnancy Pregnancy, Ectopic - blood Pregnancy, Ectopic - genetics Vascular Endothelial Growth Factor A - blood Vascular Endothelial Growth Factor A - genetics |
| title | Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T10%3A42%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Association%20study%20of%20vascular%20endothelial%20growth%20factor%20and%20polymorphisms%20of%20its%20gene%20with%20ectopic%20pregnancy&rft.jtitle=American%20journal%20of%20reproductive%20immunology%20(1989)&rft.au=Elito,%20Jr,%20Julio&rft.date=2010-02&rft.volume=63&rft.issue=2&rft.spage=120&rft.epage=125&rft.pages=120-125&rft.issn=1046-7408&rft.eissn=1600-0897&rft_id=info:doi/10.1111/j.1600-0897.2009.00769.x&rft_dat=%3Cproquest_pubme%3E745711309%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733658197&rft_id=info:pmid/20015329&rfr_iscdi=true |