Neutralizing single-chain antibodies against the tick-borne encephalitis virus
A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in Escherichia coli cells of the...
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| Veröffentlicht in: | Russian journal of bioorganic chemistry 2009-07, Vol.35 (4), p.474-481 |
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| creator | Levanov, L. N. Matveev, L. E. Yun, T. E. Goncharova, E. P. Lebedev, L. R. Shvalov, A. N. Ryzhykov, A. B. Baikov, I. K. Matveeva, V. A. Rikhter, V. A. Tikunova, N. V. |
| description | A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in
Escherichia coli
cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 10
7
M
−1
for the equilibrium state and (2.8 ± 0.3) × 10
7
M
−1
in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC
50
value for scl3D6 was 16.7 μg/ml. |
| doi_str_mv | 10.1134/S1068162009040098 |
| format | Article |
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Escherichia coli
cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 10
7
M
−1
for the equilibrium state and (2.8 ± 0.3) × 10
7
M
−1
in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC
50
value for scl3D6 was 16.7 μg/ml.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1134/S1068162009040098</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Escherichia coli ; Life Sciences ; Organic Chemistry ; Tick-borne encephalitis virus</subject><ispartof>Russian journal of bioorganic chemistry, 2009-07, Vol.35 (4), p.474-481</ispartof><rights>Pleiades Publishing, Ltd. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c272t-180b46c002dd0a9aac0e474d0c0641aca8fe561dd64dc881c464246f6538e3f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1068162009040098$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1068162009040098$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Levanov, L. N.</creatorcontrib><creatorcontrib>Matveev, L. E.</creatorcontrib><creatorcontrib>Yun, T. E.</creatorcontrib><creatorcontrib>Goncharova, E. P.</creatorcontrib><creatorcontrib>Lebedev, L. R.</creatorcontrib><creatorcontrib>Shvalov, A. N.</creatorcontrib><creatorcontrib>Ryzhykov, A. B.</creatorcontrib><creatorcontrib>Baikov, I. K.</creatorcontrib><creatorcontrib>Matveeva, V. A.</creatorcontrib><creatorcontrib>Rikhter, V. A.</creatorcontrib><creatorcontrib>Tikunova, N. V.</creatorcontrib><title>Neutralizing single-chain antibodies against the tick-borne encephalitis virus</title><title>Russian journal of bioorganic chemistry</title><addtitle>Russ J Bioorg Chem</addtitle><description>A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in
Escherichia coli
cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 10
7
M
−1
for the equilibrium state and (2.8 ± 0.3) × 10
7
M
−1
in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC
50
value for scl3D6 was 16.7 μg/ml.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Escherichia coli</subject><subject>Life Sciences</subject><subject>Organic Chemistry</subject><subject>Tick-borne encephalitis virus</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kEFPwzAMhSMEEmPwA7j1xqlgt2maHdEEDGkaB0DiFmWpu2V06UhSJPj1ZBo3JC625fc-S36MXSJcI5b85hlBSBQFwAR4KvKIjVCAzMsS3o7TnOR8r5-ysxA2AAhQyRFbLGiIXnf227pVFlLpKDdrbV2mXbTLvrEUMr1KixCzuKYsWvOeL3vvKCNnaLdOcLQh-7R-COfspNVdoIvfPmav93cv01k-f3p4nN7Oc1PURcxRwpILA1A0DeiJ1gaI17wBA4KjNlq2VAlsGsEbIyUaLnjBRSuqUlLZYjlmV4e7O99_DBSi2tpgqOu0o34IquZVDRMsZXLiwWl8H4KnVu283Wr_pRDUPjr1J7rEFAcmJK9bkVebfvAuPfQP9AOLlnDS</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Levanov, L. N.</creator><creator>Matveev, L. E.</creator><creator>Yun, T. E.</creator><creator>Goncharova, E. P.</creator><creator>Lebedev, L. R.</creator><creator>Shvalov, A. N.</creator><creator>Ryzhykov, A. B.</creator><creator>Baikov, I. K.</creator><creator>Matveeva, V. A.</creator><creator>Rikhter, V. A.</creator><creator>Tikunova, N. V.</creator><general>SP MAIK Nauka/Interperiodica</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20090701</creationdate><title>Neutralizing single-chain antibodies against the tick-borne encephalitis virus</title><author>Levanov, L. N. ; Matveev, L. E. ; Yun, T. E. ; Goncharova, E. P. ; Lebedev, L. R. ; Shvalov, A. N. ; Ryzhykov, A. B. ; Baikov, I. K. ; Matveeva, V. A. ; Rikhter, V. A. ; Tikunova, N. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-180b46c002dd0a9aac0e474d0c0641aca8fe561dd64dc881c464246f6538e3f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Escherichia coli</topic><topic>Life Sciences</topic><topic>Organic Chemistry</topic><topic>Tick-borne encephalitis virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Levanov, L. N.</creatorcontrib><creatorcontrib>Matveev, L. E.</creatorcontrib><creatorcontrib>Yun, T. E.</creatorcontrib><creatorcontrib>Goncharova, E. P.</creatorcontrib><creatorcontrib>Lebedev, L. R.</creatorcontrib><creatorcontrib>Shvalov, A. N.</creatorcontrib><creatorcontrib>Ryzhykov, A. B.</creatorcontrib><creatorcontrib>Baikov, I. K.</creatorcontrib><creatorcontrib>Matveeva, V. A.</creatorcontrib><creatorcontrib>Rikhter, V. A.</creatorcontrib><creatorcontrib>Tikunova, N. V.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Levanov, L. N.</au><au>Matveev, L. E.</au><au>Yun, T. E.</au><au>Goncharova, E. P.</au><au>Lebedev, L. R.</au><au>Shvalov, A. N.</au><au>Ryzhykov, A. B.</au><au>Baikov, I. K.</au><au>Matveeva, V. A.</au><au>Rikhter, V. A.</au><au>Tikunova, N. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutralizing single-chain antibodies against the tick-borne encephalitis virus</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2009-07-01</date><risdate>2009</risdate><volume>35</volume><issue>4</issue><spage>474</spage><epage>481</epage><pages>474-481</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in
Escherichia coli
cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 10
7
M
−1
for the equilibrium state and (2.8 ± 0.3) × 10
7
M
−1
in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC
50
value for scl3D6 was 16.7 μg/ml.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1068162009040098</doi><tpages>8</tpages></addata></record> |
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| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Escherichia coli Life Sciences Organic Chemistry Tick-borne encephalitis virus |
| title | Neutralizing single-chain antibodies against the tick-borne encephalitis virus |
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