Characterization of Water-Soluble Complexes of Polyacrylic Acid with [alpha]-Amylase from Aspergillus oryzae
Polycomplex formation of [alpha]-Amylase from Aspergillus oryzae (TAKA) with polyacrylic acid (PAA) was studied by pH titration, fluorescence, and high performance liquid chromatography (HPLC) methods in water solutions. Acording to the our results, the complex formation and its solubility were depe...
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| Veröffentlicht in: | Protein Journal 2010-02, Vol.29 (2), p.120-126 |
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| creator | Altikatoglu, Melda Tavukcuoglu, Özlem Mustafaev, Mamed |
| description | Polycomplex formation of [alpha]-Amylase from Aspergillus oryzae (TAKA) with polyacrylic acid (PAA) was studied by pH titration, fluorescence, and high performance liquid chromatography (HPLC) methods in water solutions. Acording to the our results, the complex formation and its solubility were depended on nature of enzyme and the pH of solutions. Both of them correlates isoelectric points (PI). The stability of PAA-amylase complexes was negligibly weak at pH 7 [pH > pI (isoelectric pH)]. Stable water-soluble polycomplexes were formed at pH 5 (pI ~4.5) and coexisted with free protein molecules. Insoluble complexes has been observed at pH < 4.5. The frozen storage stabilities of the obtained complexes were also studied by measuring the activities at different pH. |
| doi_str_mv | 10.1007/s10930-010-9231-y |
| format | Article |
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Acording to the our results, the complex formation and its solubility were depended on nature of enzyme and the pH of solutions. Both of them correlates isoelectric points (PI). The stability of PAA-amylase complexes was negligibly weak at pH 7 [pH > pI (isoelectric pH)]. Stable water-soluble polycomplexes were formed at pH 5 (pI ~4.5) and coexisted with free protein molecules. Insoluble complexes has been observed at pH < 4.5. The frozen storage stabilities of the obtained complexes were also studied by measuring the activities at different pH.</description><identifier>ISSN: 1572-3887</identifier><identifier>EISSN: 1573-4943</identifier><identifier>DOI: 10.1007/s10930-010-9231-y</identifier><language>eng</language><publisher>New York: Springer</publisher><subject>Acids ; Amylases ; Aspergillus oryzae ; Chromatography ; Enzymes ; Fluorescence ; High performance liquid chromatography ; Liquid chromatography ; Measurement techniques ; Proteins</subject><ispartof>Protein Journal, 2010-02, Vol.29 (2), p.120-126</ispartof><rights>COPYRIGHT 2010 Springer</rights><rights>Springer Science+Business Media, LLC 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Altikatoglu, Melda</creatorcontrib><creatorcontrib>Tavukcuoglu, Özlem</creatorcontrib><creatorcontrib>Mustafaev, Mamed</creatorcontrib><title>Characterization of Water-Soluble Complexes of Polyacrylic Acid with [alpha]-Amylase from Aspergillus oryzae</title><title>Protein Journal</title><description>Polycomplex formation of [alpha]-Amylase from Aspergillus oryzae (TAKA) with polyacrylic acid (PAA) was studied by pH titration, fluorescence, and high performance liquid chromatography (HPLC) methods in water solutions. Acording to the our results, the complex formation and its solubility were depended on nature of enzyme and the pH of solutions. Both of them correlates isoelectric points (PI). The stability of PAA-amylase complexes was negligibly weak at pH 7 [pH > pI (isoelectric pH)]. Stable water-soluble polycomplexes were formed at pH 5 (pI ~4.5) and coexisted with free protein molecules. Insoluble complexes has been observed at pH < 4.5. The frozen storage stabilities of the obtained complexes were also studied by measuring the activities at different pH.</description><subject>Acids</subject><subject>Amylases</subject><subject>Aspergillus oryzae</subject><subject>Chromatography</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>High performance liquid chromatography</subject><subject>Liquid chromatography</subject><subject>Measurement techniques</subject><subject>Proteins</subject><issn>1572-3887</issn><issn>1573-4943</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkE1LxDAQhosouK7-AG_Fi6esmXw06bEUv2BBwQUPIiVt090sabM2Ldr99UbXk7yHeWfmmWGYKLoEvACMxY0HnFKMMGCUEgpoOopmwAVFLGX0-NcTRKUUp9GZ91uMiUwFmUU236heVYPuzV4NxnWxa-JXFXL04uxYWh3nrt1Z_aX9T-vZ2UlV_WRNFWeVqeNPM2ziN2V3G_WOsnayyuu46V0bZ36n-7WxdgyT_bRX-jw6aZT1-uIvzqPV3e0qf0DLp_vHPFuidSo5UhzqkgHwhqS0VKQOhouKAVNUA6MJYYQQqoAKIRrMKpBY1qKUCRApBKXz6Pqwdte7j1H7oWiNr7S1qtNu9IVgXGDKGQ_k1T9y68a-C7cVBNIEgpIALQ7QWlldmK5xQ_hYUK1bU7lONybUMwEJJwmVjH4DzEZ3kQ</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Altikatoglu, Melda</creator><creator>Tavukcuoglu, Özlem</creator><creator>Mustafaev, Mamed</creator><general>Springer</general><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QL</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20100201</creationdate><title>Characterization of Water-Soluble Complexes of Polyacrylic Acid with [alpha]-Amylase from Aspergillus oryzae</title><author>Altikatoglu, Melda ; 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| subjects | Acids Amylases Aspergillus oryzae Chromatography Enzymes Fluorescence High performance liquid chromatography Liquid chromatography Measurement techniques Proteins |
| title | Characterization of Water-Soluble Complexes of Polyacrylic Acid with [alpha]-Amylase from Aspergillus oryzae |
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