Bacterial synthesis, purification, and solubilization of membrane protein KCNE3, a regulator of voltage-gated potassium channels

An efficient method is described for production of membrane protein KCNE3 and its isotope labeled derivatives (¹⁵N-, ¹⁵N-/¹³C-) in amounts sufficient for structural-functional investigations. The purified protein preparation within different detergent micelles was characterized using dynamic light s...

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Veröffentlicht in:	Biochemistry (Moscow) 2009-12, Vol.74 (12), p.1344-1349
Hauptverfasser:	Goncharuk, S. A, Shulga, A. A, Ermolyuk, Ya. S, Kuzmichev, P. K, Sobol, V. A, Bocharov, E. V, Chupin, V. V, Arseniev, A. S, Kirpichnikov, M. P
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Circular Dichroism Humans Life Sciences Light scattering Magnetic Resonance Spectroscopy Membranes Micelles Microbiology Molecular biology Potassium Channels, Voltage-Gated - chemistry Potassium Channels, Voltage-Gated - genetics Potassium Channels, Voltage-Gated - isolation & purification Proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Scientific method Spectroscopy
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