High throughput sequencing of microRNAs in chicken somites
High throughput Solexa sequencing technology was applied to identify microRNAs in somites of developing chicken embryos. We obtained 651 273 reads, from which 340 415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA...
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| Veröffentlicht in: | FEBS letters 2009-05, Vol.583 (9), p.1422-1426 |
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| creator | Rathjen, Tina Pais, Helio Sweetman, Dylan Moulton, Vincent Munsterberg, Andrea Dalmay, Tamas |
| description | High throughput Solexa sequencing technology was applied to identify microRNAs in somites of developing chicken embryos. We obtained 651
273 reads, from which 340
415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA candidates were identified. Accumulation of 18 of 42 sequences was confirmed by Northern blot analysis. Ten of the 18 sequences are new variants of known miRNAs and eight short RNAs are novel miRNAs. Six of these eight have not been reported by other deep sequencing projects. One of the six new miRNAs is highly enriched in somite tissue suggesting that deep sequencing of other specific tissues has the potential to identify novel tissue specific miRNAs. |
| doi_str_mv | 10.1016/j.febslet.2009.03.048 |
| format | Article |
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273 reads, from which 340
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273 reads, from which 340
415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA candidates were identified. Accumulation of 18 of 42 sequences was confirmed by Northern blot analysis. Ten of the 18 sequences are new variants of known miRNAs and eight short RNAs are novel miRNAs. Six of these eight have not been reported by other deep sequencing projects. One of the six new miRNAs is highly enriched in somite tissue suggesting that deep sequencing of other specific tissues has the potential to identify novel tissue specific miRNAs.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Chick Embryo</subject><subject>Chicken</subject><subject>Cloning, Molecular</subject><subject>Deep sequencing</subject><subject>microRNA</subject><subject>MicroRNAs - genetics</subject><subject>Somite</subject><subject>Somites - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P20AQhleoCALtTwD5VE42s9-7XBAgaCohkPpxXjnrcbKpP4LXbsW_Z6NE6q3lNBrpmXdG8xByRqGgQNXluqhxERscCwZgC-AFCHNAZtRonnOhzAcyA6Ail9ryY3IS4xpSb6g9IsfUcma0sTNyNQ_LVTauhn5arjbTmEV8mbDzoVtmfZ21wQ_9t6ebmIUu86vgf2GXxb4NI8aP5LAum4if9vWU_Hy4_3E3zx-fv3y9u3nMvQLJc1ZrrSuUpdXCMlbyskJRUeRKWWsoKgHeL4zUXBoPpeDCMOmlFsoaVlWMn5KLXe5m6NNtcXRtiB6bpuywn6LTQiqrmZDvI4FxmsjP_ySVpoYZZRIod2D6Q4wD1m4zhLYcXh0FtxXh1m4vwm1FOOAuiUhz5_sF06LF6u_U_vMJmO-AP6HB1_eluof7W_Z9a3UrFSwAE8BT1PUuCpOF3wEHF31IDrEKA_rRVX34z7VvU2-vdA</recordid><startdate>20090506</startdate><enddate>20090506</enddate><creator>Rathjen, Tina</creator><creator>Pais, Helio</creator><creator>Sweetman, Dylan</creator><creator>Moulton, Vincent</creator><creator>Munsterberg, Andrea</creator><creator>Dalmay, Tamas</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20090506</creationdate><title>High throughput sequencing of microRNAs in chicken somites</title><author>Rathjen, Tina ; Pais, Helio ; Sweetman, Dylan ; Moulton, Vincent ; Munsterberg, Andrea ; Dalmay, Tamas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6053-2f777de5a974922a3ade4d1e3669981e640ccb857358c0a434825c5746982dd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Chick Embryo</topic><topic>Chicken</topic><topic>Cloning, Molecular</topic><topic>Deep sequencing</topic><topic>microRNA</topic><topic>MicroRNAs - genetics</topic><topic>Somite</topic><topic>Somites - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rathjen, Tina</creatorcontrib><creatorcontrib>Pais, Helio</creatorcontrib><creatorcontrib>Sweetman, Dylan</creatorcontrib><creatorcontrib>Moulton, Vincent</creatorcontrib><creatorcontrib>Munsterberg, Andrea</creatorcontrib><creatorcontrib>Dalmay, Tamas</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rathjen, Tina</au><au>Pais, Helio</au><au>Sweetman, Dylan</au><au>Moulton, Vincent</au><au>Munsterberg, Andrea</au><au>Dalmay, Tamas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High throughput sequencing of microRNAs in chicken somites</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2009-05-06</date><risdate>2009</risdate><volume>583</volume><issue>9</issue><spage>1422</spage><epage>1426</epage><pages>1422-1426</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>High throughput Solexa sequencing technology was applied to identify microRNAs in somites of developing chicken embryos. We obtained 651
273 reads, from which 340
415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA candidates were identified. Accumulation of 18 of 42 sequences was confirmed by Northern blot analysis. Ten of the 18 sequences are new variants of known miRNAs and eight short RNAs are novel miRNAs. Six of these eight have not been reported by other deep sequencing projects. One of the six new miRNAs is highly enriched in somite tissue suggesting that deep sequencing of other specific tissues has the potential to identify novel tissue specific miRNAs.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>19328789</pmid><doi>10.1016/j.febslet.2009.03.048</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2009-05, Vol.583 (9), p.1422-1426 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Blotting, Northern Chick Embryo Chicken Cloning, Molecular Deep sequencing microRNA MicroRNAs - genetics Somite Somites - metabolism |
| title | High throughput sequencing of microRNAs in chicken somites |
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