Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger lipase for tallow hydrolysis
A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7±13U/g dry substrate (U/gds) at 30°C...
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| Veröffentlicht in: | Bioresource technology 2010-09, Vol.101 (17), p.6791-6796 |
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| container_title | Bioresource technology |
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| creator | Edwinoliver, N.G. Thirunavukarasu, K. Naidu, R.B. Gowthaman, M.K. Kambe, T. Nakajima Kamini, N.R. |
| description | A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7±13U/g dry substrate (U/gds) at 30°C and 96h and growth studies indicated that addition of WR significantly augmented the biomass and lipase production. Scale up of lipase production at 100g and 3kg (3×1kg) tray-level batch fermentation resulted in 96% and 83.0% of enzyme activities, respectively, at 72h. Maximum activity of 745.7±11U/gds was obtained, when fermented substrate was extracted in buffer containing 1% (w/v) sodium chloride and 0.5% (w/v) Triton X-100. Furthermore, the direct application of fermented substrate for tallow hydrolysis makes the process economical for industrial production of biofuel. |
| doi_str_mv | 10.1016/j.biortech.2010.03.091 |
| format | Article |
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Nakajima ; Kamini, N.R.</creator><creatorcontrib>Edwinoliver, N.G. ; Thirunavukarasu, K. ; Naidu, R.B. ; Gowthaman, M.K. ; Kambe, T. Nakajima ; Kamini, N.R.</creatorcontrib><description>A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7±13U/g dry substrate (U/gds) at 30°C and 96h and growth studies indicated that addition of WR significantly augmented the biomass and lipase production. Scale up of lipase production at 100g and 3kg (3×1kg) tray-level batch fermentation resulted in 96% and 83.0% of enzyme activities, respectively, at 72h. Maximum activity of 745.7±11U/gds was obtained, when fermented substrate was extracted in buffer containing 1% (w/v) sodium chloride and 0.5% (w/v) Triton X-100. Furthermore, the direct application of fermented substrate for tallow hydrolysis makes the process economical for industrial production of biofuel.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2010.03.091</identifier><identifier>PMID: 20400303</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Aspergillus niger ; Aspergillus niger - enzymology ; Biological and medical sciences ; Biomass ; Biotechnology ; Fats - metabolism ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Lipase ; Lipase - biosynthesis ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Scanning electron microscopy ; Substrate Specificity ; Tallow hydrolysis ; Tri-substrate fermentation ; Triticum aestivum</subject><ispartof>Bioresource technology, 2010-09, Vol.101 (17), p.6791-6796</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>(c) 2010 Elsevier Ltd. 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Nakajima</creatorcontrib><creatorcontrib>Kamini, N.R.</creatorcontrib><title>Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger lipase for tallow hydrolysis</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7±13U/g dry substrate (U/gds) at 30°C and 96h and growth studies indicated that addition of WR significantly augmented the biomass and lipase production. Scale up of lipase production at 100g and 3kg (3×1kg) tray-level batch fermentation resulted in 96% and 83.0% of enzyme activities, respectively, at 72h. Maximum activity of 745.7±11U/gds was obtained, when fermented substrate was extracted in buffer containing 1% (w/v) sodium chloride and 0.5% (w/v) Triton X-100. Furthermore, the direct application of fermented substrate for tallow hydrolysis makes the process economical for industrial production of biofuel.</description><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Fats - metabolism</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Lipase</subject><subject>Lipase - biosynthesis</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Scanning electron microscopy</subject><subject>Substrate Specificity</subject><subject>Tallow hydrolysis</subject><subject>Tri-substrate fermentation</subject><subject>Triticum aestivum</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtvEzEURi0EoqHwF4o3iNUEP8eeHVXFS6rEonRteTzXiSNnHOyZlvx7nEdhCQvL1tX5fK_uQeiKkiUltP2wWfYh5QnceslILRK-JB19hhZUK96wTrXP0YJ0LWm0ZOICvSplQwjhVLGX6IIRUd-EL9CvO2cj4HmHk8cWj-kBIp5yaMrclynbCbCHvIVxslNII_YpYxjXdnQw4F1Ow-yO9Zq-LjvIqxDjXPAYVpBxDDtb4JiZbIzpEa_3Q05xX0J5jV54Gwu8Od-X6P7zpx83X5vb71--3VzfNk7Sbmr6oVVOSS1lqyWXWvTEt44J4a1VkmnFiJV0cJ11UlPHhrYFXRPOc6UcVfwSvT_9W4f9OUOZzDYUBzHaEdJcjBKyFUKR_yA5l3WETlSyPZEup1IyeLPLYWvz3lBiDnrMxjzpMQc9hnBT9dTg1bnF3G9h-BN78lGBd2fAlirG57roUP5yTHPNjhO8PXHeJmNXuTL3d7UTJ1QLUU8lPp4IqMt9CJBNcQEO2kIGN5khhX9N-xvdT7vS</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Edwinoliver, N.G.</creator><creator>Thirunavukarasu, K.</creator><creator>Naidu, R.B.</creator><creator>Gowthaman, M.K.</creator><creator>Kambe, T. 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| subjects | Aspergillus niger Aspergillus niger - enzymology Biological and medical sciences Biomass Biotechnology Fats - metabolism Fermentation Fundamental and applied biological sciences. Psychology Hydrolysis Lipase Lipase - biosynthesis Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Scanning electron microscopy Substrate Specificity Tallow hydrolysis Tri-substrate fermentation Triticum aestivum |
| title | Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger lipase for tallow hydrolysis |
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