Separation of cytoplasts and whole cells using density gradients of renografin
Cytoplasts prepared from L929 or Hepa‐2 cells were separated from whole cells using density gradients of renografin. Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vital dye trypan...
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| Veröffentlicht in: | Journal of cellular physiology 1979-03, Vol.98 (3), p.637-642 |
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| container_end_page | 642 |
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| container_issue | 3 |
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| container_title | Journal of cellular physiology |
| container_volume | 98 |
| creator | Lipsich, Leah Ann Lucas, Joseph J. Kates, Joseph R. |
| description | Cytoplasts prepared from L929 or Hepa‐2 cells were separated from whole cells using density gradients of renografin. Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vital dye trypan blue and were utilized in nuclear transplantation experiments to reconstruct whole viable cells capable of division. In addition, the renografin gradient technique was used to separate the newly reconstructed cells from any contaminating “non‐renucleated” cytoplasts. This will permit immediate biochemical characterization of cytoplasmic‐nuclear hybrid cells without interference from contaminating cytoplasts. |
| doi_str_mv | 10.1002/jcp.1040980322 |
| format | Article |
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Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vital dye trypan blue and were utilized in nuclear transplantation experiments to reconstruct whole viable cells capable of division. In addition, the renografin gradient technique was used to separate the newly reconstructed cells from any contaminating “non‐renucleated” cytoplasts. This will permit immediate biochemical characterization of cytoplasmic‐nuclear hybrid cells without interference from contaminating cytoplasts.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.1040980322</identifier><identifier>PMID: 438307</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Cell Fractionation - methods ; Cell Line ; Cell Nucleus - ultrastructure ; Cell Separation - methods ; Centrifugation, Density Gradient ; Cytoplasm - ultrastructure ; Diatrizoate Meglumine ; Hybrid Cells - ultrastructure ; L Cells (Cell Line) - ultrastructure ; Liver Neoplasms, Experimental - ultrastructure ; Mice</subject><ispartof>Journal of cellular physiology, 1979-03, Vol.98 (3), p.637-642</ispartof><rights>Copyright © 1979 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3772-550ba3dc273b2539ad7b2258fea8c7044c8ed998d04527e48a33db858c37cd883</citedby><cites>FETCH-LOGICAL-c3772-550ba3dc273b2539ad7b2258fea8c7044c8ed998d04527e48a33db858c37cd883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.1040980322$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.1040980322$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/438307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lipsich, Leah Ann</creatorcontrib><creatorcontrib>Lucas, Joseph J.</creatorcontrib><creatorcontrib>Kates, Joseph R.</creatorcontrib><title>Separation of cytoplasts and whole cells using density gradients of renografin</title><title>Journal of cellular physiology</title><addtitle>J. Cell. Physiol</addtitle><description>Cytoplasts prepared from L929 or Hepa‐2 cells were separated from whole cells using density gradients of renografin. Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vital dye trypan blue and were utilized in nuclear transplantation experiments to reconstruct whole viable cells capable of division. In addition, the renografin gradient technique was used to separate the newly reconstructed cells from any contaminating “non‐renucleated” cytoplasts. This will permit immediate biochemical characterization of cytoplasmic‐nuclear hybrid cells without interference from contaminating cytoplasts.</description><subject>Animals</subject><subject>Cell Fractionation - methods</subject><subject>Cell Line</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cell Separation - methods</subject><subject>Centrifugation, Density Gradient</subject><subject>Cytoplasm - ultrastructure</subject><subject>Diatrizoate Meglumine</subject><subject>Hybrid Cells - ultrastructure</subject><subject>L Cells (Cell Line) - ultrastructure</subject><subject>Liver Neoplasms, Experimental - ultrastructure</subject><subject>Mice</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAURi3EqzxWJoZMbAHHj9oeUaEFhADxHC3HdopLmgQ7Uem_xyhVERPTte4955P1AXCUwdMMQnQ20018ECg4xAhtgEEGBUvJkKJNMIhAlgpKsl2wF8IMQigExjtgm2COIRuAuyfbKK9aV1dJXSR62dZNqUIbElWZZPFelzbRtixD0gVXTRNjq-DaZTL1yjhbRS5a3lZ1XBSuOgBbhSqDPVzNffAyvnweXaW395Pr0fltqjFjKKUU5gobjRjOEcVCGZYjRHlhFdcMEqK5NUJwAwlFzBKuMDY5pzzq2nCO98FJn9v4-rOzoZVzF37-qSpbd0EyQocEIRLB0x7Uvg7B20I23s2VX8oMyp_-ZOxP_vYXheNVcpfPrVnjfWHxLPrzwpV2-U-YvBk9_IlOe9eF1n6tXeU_5JBhRuXb3USysRi-InYhH_E3yX2LKA</recordid><startdate>197903</startdate><enddate>197903</enddate><creator>Lipsich, Leah Ann</creator><creator>Lucas, Joseph J.</creator><creator>Kates, Joseph R.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197903</creationdate><title>Separation of cytoplasts and whole cells using density gradients of renografin</title><author>Lipsich, Leah Ann ; Lucas, Joseph J. ; Kates, Joseph R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3772-550ba3dc273b2539ad7b2258fea8c7044c8ed998d04527e48a33db858c37cd883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Cell Fractionation - methods</topic><topic>Cell Line</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cell Separation - methods</topic><topic>Centrifugation, Density Gradient</topic><topic>Cytoplasm - ultrastructure</topic><topic>Diatrizoate Meglumine</topic><topic>Hybrid Cells - ultrastructure</topic><topic>L Cells (Cell Line) - ultrastructure</topic><topic>Liver Neoplasms, Experimental - ultrastructure</topic><topic>Mice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lipsich, Leah Ann</creatorcontrib><creatorcontrib>Lucas, Joseph J.</creatorcontrib><creatorcontrib>Kates, Joseph R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lipsich, Leah Ann</au><au>Lucas, Joseph J.</au><au>Kates, Joseph R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of cytoplasts and whole cells using density gradients of renografin</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J. Cell. Physiol</addtitle><date>1979-03</date><risdate>1979</risdate><volume>98</volume><issue>3</issue><spage>637</spage><epage>642</epage><pages>637-642</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Cytoplasts prepared from L929 or Hepa‐2 cells were separated from whole cells using density gradients of renografin. Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vital dye trypan blue and were utilized in nuclear transplantation experiments to reconstruct whole viable cells capable of division. In addition, the renografin gradient technique was used to separate the newly reconstructed cells from any contaminating “non‐renucleated” cytoplasts. This will permit immediate biochemical characterization of cytoplasmic‐nuclear hybrid cells without interference from contaminating cytoplasts.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>438307</pmid><doi>10.1002/jcp.1040980322</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9541 |
| ispartof | Journal of cellular physiology, 1979-03, Vol.98 (3), p.637-642 |
| issn | 0021-9541 1097-4652 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals |
| subjects | Animals Cell Fractionation - methods Cell Line Cell Nucleus - ultrastructure Cell Separation - methods Centrifugation, Density Gradient Cytoplasm - ultrastructure Diatrizoate Meglumine Hybrid Cells - ultrastructure L Cells (Cell Line) - ultrastructure Liver Neoplasms, Experimental - ultrastructure Mice |
| title | Separation of cytoplasts and whole cells using density gradients of renografin |
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