Induction of chemoresistance in a cultured canine cell line by retroviral transduction of the canine multidrug resistance 1 gene
To induce chemoresistance in a normal canine cell line through the transduction of the canine multidrug resistance 1 gene (mdr1). Madin-Darby canine kidney (MDCK) epithelial cell line. The full-length canine mdr1 cDNA clone isolated in our laboratory was inserted into a Moloney murine leukemia virus...
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| Veröffentlicht in: | American journal of veterinary research 2007, Vol.68 (1), p.95-100 |
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| creator | Matsuura, S Koto, H Ide, K Fujino, Y Setoguchi-Mukai, A Ohno, K Tsujimoto, H |
| description | To induce chemoresistance in a normal canine cell line through the transduction of the canine multidrug resistance 1 gene (mdr1).
Madin-Darby canine kidney (MDCK) epithelial cell line.
The full-length canine mdr1 cDNA clone isolated in our laboratory was inserted into a Moloney murine leukemia virus-based vector to construct the retroviral vector, pLNC-cMDR1. After retroviral transduction of pLNC-cMDR1 into MDCK cells, the expression and function of the P-glycoprotein, a product of mdr1, were assessed by immunoblotting, measurement of rhodamine123 (Rh123) retention, and drug sensitivity assays.
P-glycoprotein was strongly expressed in cells transduced with pLNC-cMDR1. This P-glycoprotein was fully functional, as demonstrated by the decreased Rh123 retention and the increased resistance to chemotherapeutic drugs. Measured as 50% inhibitory concentrations, resistance increased 59 times to vincristine and 25 times to doxorubicin in MDCK cells after transduction of pLNC-cMDR1.
Transduction of canine mdr1 is an effective method for inducing chemoresistance in normal canine cells. This system may be applicable to the induction of drug resistance in hematopoietic cells. |
| doi_str_mv | 10.2460/ajvr.68.1.95 |
| format | Article |
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Madin-Darby canine kidney (MDCK) epithelial cell line.
The full-length canine mdr1 cDNA clone isolated in our laboratory was inserted into a Moloney murine leukemia virus-based vector to construct the retroviral vector, pLNC-cMDR1. After retroviral transduction of pLNC-cMDR1 into MDCK cells, the expression and function of the P-glycoprotein, a product of mdr1, were assessed by immunoblotting, measurement of rhodamine123 (Rh123) retention, and drug sensitivity assays.
P-glycoprotein was strongly expressed in cells transduced with pLNC-cMDR1. This P-glycoprotein was fully functional, as demonstrated by the decreased Rh123 retention and the increased resistance to chemotherapeutic drugs. Measured as 50% inhibitory concentrations, resistance increased 59 times to vincristine and 25 times to doxorubicin in MDCK cells after transduction of pLNC-cMDR1.
Transduction of canine mdr1 is an effective method for inducing chemoresistance in normal canine cells. This system may be applicable to the induction of drug resistance in hematopoietic cells.</description><identifier>ISSN: 0002-9645</identifier><identifier>EISSN: 1943-5681</identifier><identifier>DOI: 10.2460/ajvr.68.1.95</identifier><identifier>PMID: 17199425</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antineoplastic Agents - pharmacology ; ATP-Binding Cassette, Sub-Family B, Member 1 - biosynthesis ; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Base Sequence ; Cell Line ; cell lines ; Cercopithecus aethiops ; complementary DNA ; COS Cells ; cultured cells ; DNA, Complementary - genetics ; dogs ; Dogs - genetics ; Dogs - metabolism ; Doxorubicin - pharmacology ; drug resistance ; Drug Resistance, Neoplasm ; epithelium ; Flow Cytometry - veterinary ; Fluorescent Dyes - metabolism ; genes ; genetic transduction ; Genetic Vectors - genetics ; Inhibitory Concentration 50 ; Molecular Sequence Data ; multiple drug resistance ; retroviral vectors ; Retroviridae - genetics ; Rhodamine 123 - metabolism ; Sequence Analysis, DNA ; Transduction, Genetic - veterinary ; Vincristine - pharmacology</subject><ispartof>American journal of veterinary research, 2007, Vol.68 (1), p.95-100</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-8e16576d8239d5bda01ab3c83232b61221fb9b768580d1fa40ee116cccb9fbfd3</citedby><cites>FETCH-LOGICAL-c449t-8e16576d8239d5bda01ab3c83232b61221fb9b768580d1fa40ee116cccb9fbfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17199425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuura, S</creatorcontrib><creatorcontrib>Koto, H</creatorcontrib><creatorcontrib>Ide, K</creatorcontrib><creatorcontrib>Fujino, Y</creatorcontrib><creatorcontrib>Setoguchi-Mukai, A</creatorcontrib><creatorcontrib>Ohno, K</creatorcontrib><creatorcontrib>Tsujimoto, H</creatorcontrib><title>Induction of chemoresistance in a cultured canine cell line by retroviral transduction of the canine multidrug resistance 1 gene</title><title>American journal of veterinary research</title><addtitle>Am J Vet Res</addtitle><description>To induce chemoresistance in a normal canine cell line through the transduction of the canine multidrug resistance 1 gene (mdr1).
Madin-Darby canine kidney (MDCK) epithelial cell line.
The full-length canine mdr1 cDNA clone isolated in our laboratory was inserted into a Moloney murine leukemia virus-based vector to construct the retroviral vector, pLNC-cMDR1. After retroviral transduction of pLNC-cMDR1 into MDCK cells, the expression and function of the P-glycoprotein, a product of mdr1, were assessed by immunoblotting, measurement of rhodamine123 (Rh123) retention, and drug sensitivity assays.
P-glycoprotein was strongly expressed in cells transduced with pLNC-cMDR1. This P-glycoprotein was fully functional, as demonstrated by the decreased Rh123 retention and the increased resistance to chemotherapeutic drugs. Measured as 50% inhibitory concentrations, resistance increased 59 times to vincristine and 25 times to doxorubicin in MDCK cells after transduction of pLNC-cMDR1.
Transduction of canine mdr1 is an effective method for inducing chemoresistance in normal canine cells. This system may be applicable to the induction of drug resistance in hematopoietic cells.</description><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - biosynthesis</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - genetics</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>cell lines</subject><subject>Cercopithecus aethiops</subject><subject>complementary DNA</subject><subject>COS Cells</subject><subject>cultured cells</subject><subject>DNA, Complementary - genetics</subject><subject>dogs</subject><subject>Dogs - genetics</subject><subject>Dogs - metabolism</subject><subject>Doxorubicin - pharmacology</subject><subject>drug resistance</subject><subject>Drug Resistance, Neoplasm</subject><subject>epithelium</subject><subject>Flow Cytometry - veterinary</subject><subject>Fluorescent Dyes - metabolism</subject><subject>genes</subject><subject>genetic transduction</subject><subject>Genetic Vectors - genetics</subject><subject>Inhibitory Concentration 50</subject><subject>Molecular Sequence Data</subject><subject>multiple drug resistance</subject><subject>retroviral vectors</subject><subject>Retroviridae - genetics</subject><subject>Rhodamine 123 - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Transduction, Genetic - veterinary</subject><subject>Vincristine - pharmacology</subject><issn>0002-9645</issn><issn>1943-5681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkD1PHDEQQK0oUThIutTEHQ178Xhtr11GiAQkJIqE2vLH7GG06yX2LhIdPz13ukNQzRRvnjSPkG_A1lwo9sM9PJW10mtYG_mBrMCItpFKw0eyYozxxighj8hxrQ-MAdcgP5Mj6MAYweWKvFznuIQ5TZlOPQ33OE4Fa6qzywFpytTRsAzzUjDS4HLKSAMOAx12m3-mBecyPaXiBjoXl-s72XyPryfjVpFiWTb0nRzoBjN-IZ96N1T8epgn5O7X5d-Lq-bm9vf1xc-bJghh5kYjKNmpqHlrovTRMXC-DbrlLfcKOIfeG98pLTWL0DvBEAFUCMGb3vexPSFne-9jmf4tWGc7prp7xWWclmo7IZVgnTFb8nxPhjLVWrC3jyWNrjxbYHaX3O6SW6UtWCO3-OlBvPgR4xt8aLwFvu-B3k3WbUqq9u4PZ9Ay1gnDTdf-By8YiQw</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Matsuura, S</creator><creator>Koto, H</creator><creator>Ide, K</creator><creator>Fujino, Y</creator><creator>Setoguchi-Mukai, A</creator><creator>Ohno, K</creator><creator>Tsujimoto, H</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>2007</creationdate><title>Induction of chemoresistance in a cultured canine cell line by retroviral transduction of the canine multidrug resistance 1 gene</title><author>Matsuura, S ; Koto, H ; Ide, K ; Fujino, Y ; Setoguchi-Mukai, A ; Ohno, K ; Tsujimoto, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-8e16576d8239d5bda01ab3c83232b61221fb9b768580d1fa40ee116cccb9fbfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - biosynthesis</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - genetics</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>cell lines</topic><topic>Cercopithecus aethiops</topic><topic>complementary DNA</topic><topic>COS Cells</topic><topic>cultured cells</topic><topic>DNA, Complementary - genetics</topic><topic>dogs</topic><topic>Dogs - genetics</topic><topic>Dogs - metabolism</topic><topic>Doxorubicin - pharmacology</topic><topic>drug resistance</topic><topic>Drug Resistance, Neoplasm</topic><topic>epithelium</topic><topic>Flow Cytometry - veterinary</topic><topic>Fluorescent Dyes - metabolism</topic><topic>genes</topic><topic>genetic transduction</topic><topic>Genetic Vectors - genetics</topic><topic>Inhibitory Concentration 50</topic><topic>Molecular Sequence Data</topic><topic>multiple drug resistance</topic><topic>retroviral vectors</topic><topic>Retroviridae - genetics</topic><topic>Rhodamine 123 - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Transduction, Genetic - veterinary</topic><topic>Vincristine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuura, S</creatorcontrib><creatorcontrib>Koto, H</creatorcontrib><creatorcontrib>Ide, K</creatorcontrib><creatorcontrib>Fujino, Y</creatorcontrib><creatorcontrib>Setoguchi-Mukai, A</creatorcontrib><creatorcontrib>Ohno, K</creatorcontrib><creatorcontrib>Tsujimoto, H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>American journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuura, S</au><au>Koto, H</au><au>Ide, K</au><au>Fujino, Y</au><au>Setoguchi-Mukai, A</au><au>Ohno, K</au><au>Tsujimoto, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of chemoresistance in a cultured canine cell line by retroviral transduction of the canine multidrug resistance 1 gene</atitle><jtitle>American journal of veterinary research</jtitle><addtitle>Am J Vet Res</addtitle><date>2007</date><risdate>2007</risdate><volume>68</volume><issue>1</issue><spage>95</spage><epage>100</epage><pages>95-100</pages><issn>0002-9645</issn><eissn>1943-5681</eissn><abstract>To induce chemoresistance in a normal canine cell line through the transduction of the canine multidrug resistance 1 gene (mdr1).
Madin-Darby canine kidney (MDCK) epithelial cell line.
The full-length canine mdr1 cDNA clone isolated in our laboratory was inserted into a Moloney murine leukemia virus-based vector to construct the retroviral vector, pLNC-cMDR1. After retroviral transduction of pLNC-cMDR1 into MDCK cells, the expression and function of the P-glycoprotein, a product of mdr1, were assessed by immunoblotting, measurement of rhodamine123 (Rh123) retention, and drug sensitivity assays.
P-glycoprotein was strongly expressed in cells transduced with pLNC-cMDR1. This P-glycoprotein was fully functional, as demonstrated by the decreased Rh123 retention and the increased resistance to chemotherapeutic drugs. Measured as 50% inhibitory concentrations, resistance increased 59 times to vincristine and 25 times to doxorubicin in MDCK cells after transduction of pLNC-cMDR1.
Transduction of canine mdr1 is an effective method for inducing chemoresistance in normal canine cells. This system may be applicable to the induction of drug resistance in hematopoietic cells.</abstract><cop>United States</cop><pmid>17199425</pmid><doi>10.2460/ajvr.68.1.95</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antineoplastic Agents - pharmacology ATP-Binding Cassette, Sub-Family B, Member 1 - biosynthesis ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Base Sequence Cell Line cell lines Cercopithecus aethiops complementary DNA COS Cells cultured cells DNA, Complementary - genetics dogs Dogs - genetics Dogs - metabolism Doxorubicin - pharmacology drug resistance Drug Resistance, Neoplasm epithelium Flow Cytometry - veterinary Fluorescent Dyes - metabolism genes genetic transduction Genetic Vectors - genetics Inhibitory Concentration 50 Molecular Sequence Data multiple drug resistance retroviral vectors Retroviridae - genetics Rhodamine 123 - metabolism Sequence Analysis, DNA Transduction, Genetic - veterinary Vincristine - pharmacology |
| title | Induction of chemoresistance in a cultured canine cell line by retroviral transduction of the canine multidrug resistance 1 gene |
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