Comparison of distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma, or histologically normal urinary bladders
To compare distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma (TCC), or histologically normal urinary bladders. 24 archived and 7 fresh-frozen specimens of urinary bladders from dogs with cystitis. Immunohistochemical analysis of archived...
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| Veröffentlicht in: | American journal of veterinary research 2008-08, Vol.69 (8), p.1073-1078 |
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| creator | Rankin, W.V Henry, C.J Turnquist, S.E Turk, J.R Beissenherz, M.E Tyler, J.W Green, J.A |
| description | To compare distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma (TCC), or histologically normal urinary bladders.
24 archived and 7 fresh-frozen specimens of urinary bladders from dogs with cystitis.
Immunohistochemical analysis of archived tissue specimens was performed to identify survivin protein in the nucleus and cytoplasm of cells by use of polyclonal rabbit anti-survivin antibody. Tissues that contained > or = 5% immunoreactive cells were considered positive for survivin protein. Reverse-transcription PCR analysis was performed on fresh-frozen tissues to identify survivin mRNA. Data on tissues from dogs with TCC or histologically normal urinary bladders that were obtained during another study were used for statistical comparisons.
Twelve of 24 (50%) cystitic tissues were positive for nuclear survivin, compared with 28 of 41 (68%) TCC tissues and 0 of 46 (0%) normal tissues. Two of 24 (8%) cystitic tissues were positive for cytoplasmic survivin, compared with 7 of 41 (17%) TCC tissues and 17 of 46 (37%) normal tissues. Proportions of specimens that contained nuclear or cytoplasmic survivin were significantly different between cystitic and normal tissues but not between cystitic and TCC tissues. Four of 7 cystitic tissues were positive for survivin mRNA, which was comparable with results for TCC and normal tissues.
Nuclear survivin was detected in TCC and cystitic tissues but not in normal urinary bladder tissues. Additional studies are needed to determine whether nuclear survivin contributes to the development or progression of TCC. |
| doi_str_mv | 10.2460/ajvr.69.8.1073 |
| format | Article |
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24 archived and 7 fresh-frozen specimens of urinary bladders from dogs with cystitis.
Immunohistochemical analysis of archived tissue specimens was performed to identify survivin protein in the nucleus and cytoplasm of cells by use of polyclonal rabbit anti-survivin antibody. Tissues that contained > or = 5% immunoreactive cells were considered positive for survivin protein. Reverse-transcription PCR analysis was performed on fresh-frozen tissues to identify survivin mRNA. Data on tissues from dogs with TCC or histologically normal urinary bladders that were obtained during another study were used for statistical comparisons.
Twelve of 24 (50%) cystitic tissues were positive for nuclear survivin, compared with 28 of 41 (68%) TCC tissues and 0 of 46 (0%) normal tissues. Two of 24 (8%) cystitic tissues were positive for cytoplasmic survivin, compared with 7 of 41 (17%) TCC tissues and 17 of 46 (37%) normal tissues. Proportions of specimens that contained nuclear or cytoplasmic survivin were significantly different between cystitic and normal tissues but not between cystitic and TCC tissues. Four of 7 cystitic tissues were positive for survivin mRNA, which was comparable with results for TCC and normal tissues.
Nuclear survivin was detected in TCC and cystitic tissues but not in normal urinary bladder tissues. Additional studies are needed to determine whether nuclear survivin contributes to the development or progression of TCC.</description><identifier>ISSN: 0002-9645</identifier><identifier>EISSN: 1943-5681</identifier><identifier>DOI: 10.2460/ajvr.69.8.1073</identifier><identifier>PMID: 18672973</identifier><language>eng</language><publisher>United States</publisher><subject>animal proteins ; Animals ; apoptosis ; bladder ; bladder diseases ; carcinoma ; Carcinoma, Transitional Cell - metabolism ; Carcinoma, Transitional Cell - veterinary ; cell nucleus ; Cell Nucleus - metabolism ; cell proliferation ; cystitis ; Cystitis - metabolism ; Cystitis - veterinary ; cytoplasm ; dog diseases ; Dog Diseases - metabolism ; Dogs ; histology ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; neoplasms ; normal values ; pathogenesis ; Reference Values ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; survivin ; tissue analysis ; transitional cell carcinoma ; Urinary Bladder - metabolism ; Urinary Bladder Neoplasms - metabolism ; Urinary Bladder Neoplasms - veterinary</subject><ispartof>American journal of veterinary research, 2008-08, Vol.69 (8), p.1073-1078</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ab20b7a395295ac2e7604e76dab3183ca7151a4f0122227c1b6b73c7f5c0ff3a3</citedby><cites>FETCH-LOGICAL-c389t-ab20b7a395295ac2e7604e76dab3183ca7151a4f0122227c1b6b73c7f5c0ff3a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18672973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rankin, W.V</creatorcontrib><creatorcontrib>Henry, C.J</creatorcontrib><creatorcontrib>Turnquist, S.E</creatorcontrib><creatorcontrib>Turk, J.R</creatorcontrib><creatorcontrib>Beissenherz, M.E</creatorcontrib><creatorcontrib>Tyler, J.W</creatorcontrib><creatorcontrib>Green, J.A</creatorcontrib><title>Comparison of distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma, or histologically normal urinary bladders</title><title>American journal of veterinary research</title><addtitle>Am J Vet Res</addtitle><description>To compare distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma (TCC), or histologically normal urinary bladders.
24 archived and 7 fresh-frozen specimens of urinary bladders from dogs with cystitis.
Immunohistochemical analysis of archived tissue specimens was performed to identify survivin protein in the nucleus and cytoplasm of cells by use of polyclonal rabbit anti-survivin antibody. Tissues that contained > or = 5% immunoreactive cells were considered positive for survivin protein. Reverse-transcription PCR analysis was performed on fresh-frozen tissues to identify survivin mRNA. Data on tissues from dogs with TCC or histologically normal urinary bladders that were obtained during another study were used for statistical comparisons.
Twelve of 24 (50%) cystitic tissues were positive for nuclear survivin, compared with 28 of 41 (68%) TCC tissues and 0 of 46 (0%) normal tissues. Two of 24 (8%) cystitic tissues were positive for cytoplasmic survivin, compared with 7 of 41 (17%) TCC tissues and 17 of 46 (37%) normal tissues. Proportions of specimens that contained nuclear or cytoplasmic survivin were significantly different between cystitic and normal tissues but not between cystitic and TCC tissues. Four of 7 cystitic tissues were positive for survivin mRNA, which was comparable with results for TCC and normal tissues.
Nuclear survivin was detected in TCC and cystitic tissues but not in normal urinary bladder tissues. Additional studies are needed to determine whether nuclear survivin contributes to the development or progression of TCC.</description><subject>animal proteins</subject><subject>Animals</subject><subject>apoptosis</subject><subject>bladder</subject><subject>bladder diseases</subject><subject>carcinoma</subject><subject>Carcinoma, Transitional Cell - metabolism</subject><subject>Carcinoma, Transitional Cell - veterinary</subject><subject>cell nucleus</subject><subject>Cell Nucleus - metabolism</subject><subject>cell proliferation</subject><subject>cystitis</subject><subject>Cystitis - metabolism</subject><subject>Cystitis - veterinary</subject><subject>cytoplasm</subject><subject>dog diseases</subject><subject>Dog Diseases - metabolism</subject><subject>Dogs</subject><subject>histology</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>neoplasms</subject><subject>normal values</subject><subject>pathogenesis</subject><subject>Reference Values</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>survivin</subject><subject>tissue analysis</subject><subject>transitional cell carcinoma</subject><subject>Urinary Bladder - metabolism</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><subject>Urinary Bladder Neoplasms - veterinary</subject><issn>0002-9645</issn><issn>1943-5681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv3CAURlGVqpmm3XbZsko3scvDBrOMRulDipRFmjW6xmZCZMME7InmJ-VfBndGqpRFQLpI6PDB5SD0hZKSVYL8gIddLIUqm5ISyd-hFVUVL2rR0BO0IoSwQomqPkUfU3oghLKG1h_QKW2EZEryFXpeh3EL0aXgcbC4c2mKrp0nF3xaNtIcd27nPIYx-A2eXEpzn7CNYcRzdB7iHrcDdF0f__Fd2CT85KZ7bPZpcpm_wFMEn9wSCQM2_ZALRON8GOECh4jv86VhCBtnYBj22Ic4ZvB1-if03sKQ-s_H9Qzd_bz6u_5dXN_8-rO-vC4Mb9RUQMtIK4GrmqkaDOulIFUuHbScNtyApDWFyua_yEMa2opWciNtbYi1HPgZ-n7I3cbwmHud9OjS8mrwfZiTllUtuMozk-dvkkJxKQWlGSwPoIkhpdhbvY1uzM1pSvSiUS8aM68bvWjMB74ek-d27Lv_-NFbBr4dAAtBwyb703e3jFBOiGJCMcJfABi3p54</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Rankin, W.V</creator><creator>Henry, C.J</creator><creator>Turnquist, S.E</creator><creator>Turk, J.R</creator><creator>Beissenherz, M.E</creator><creator>Tyler, J.W</creator><creator>Green, J.A</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20080801</creationdate><title>Comparison of distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma, or histologically normal urinary bladders</title><author>Rankin, W.V ; Henry, C.J ; Turnquist, S.E ; Turk, J.R ; Beissenherz, M.E ; Tyler, J.W ; Green, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ab20b7a395295ac2e7604e76dab3183ca7151a4f0122227c1b6b73c7f5c0ff3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>animal proteins</topic><topic>Animals</topic><topic>apoptosis</topic><topic>bladder</topic><topic>bladder diseases</topic><topic>carcinoma</topic><topic>Carcinoma, Transitional Cell - metabolism</topic><topic>Carcinoma, Transitional Cell - veterinary</topic><topic>cell nucleus</topic><topic>Cell Nucleus - metabolism</topic><topic>cell proliferation</topic><topic>cystitis</topic><topic>Cystitis - metabolism</topic><topic>Cystitis - veterinary</topic><topic>cytoplasm</topic><topic>dog diseases</topic><topic>Dog Diseases - metabolism</topic><topic>Dogs</topic><topic>histology</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>neoplasms</topic><topic>normal values</topic><topic>pathogenesis</topic><topic>Reference Values</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>survivin</topic><topic>tissue analysis</topic><topic>transitional cell carcinoma</topic><topic>Urinary Bladder - metabolism</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><topic>Urinary Bladder Neoplasms - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rankin, W.V</creatorcontrib><creatorcontrib>Henry, C.J</creatorcontrib><creatorcontrib>Turnquist, S.E</creatorcontrib><creatorcontrib>Turk, J.R</creatorcontrib><creatorcontrib>Beissenherz, M.E</creatorcontrib><creatorcontrib>Tyler, J.W</creatorcontrib><creatorcontrib>Green, J.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>American journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rankin, W.V</au><au>Henry, C.J</au><au>Turnquist, S.E</au><au>Turk, J.R</au><au>Beissenherz, M.E</au><au>Tyler, J.W</au><au>Green, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma, or histologically normal urinary bladders</atitle><jtitle>American journal of veterinary research</jtitle><addtitle>Am J Vet Res</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>69</volume><issue>8</issue><spage>1073</spage><epage>1078</epage><pages>1073-1078</pages><issn>0002-9645</issn><eissn>1943-5681</eissn><abstract>To compare distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma (TCC), or histologically normal urinary bladders.
24 archived and 7 fresh-frozen specimens of urinary bladders from dogs with cystitis.
Immunohistochemical analysis of archived tissue specimens was performed to identify survivin protein in the nucleus and cytoplasm of cells by use of polyclonal rabbit anti-survivin antibody. Tissues that contained > or = 5% immunoreactive cells were considered positive for survivin protein. Reverse-transcription PCR analysis was performed on fresh-frozen tissues to identify survivin mRNA. Data on tissues from dogs with TCC or histologically normal urinary bladders that were obtained during another study were used for statistical comparisons.
Twelve of 24 (50%) cystitic tissues were positive for nuclear survivin, compared with 28 of 41 (68%) TCC tissues and 0 of 46 (0%) normal tissues. Two of 24 (8%) cystitic tissues were positive for cytoplasmic survivin, compared with 7 of 41 (17%) TCC tissues and 17 of 46 (37%) normal tissues. Proportions of specimens that contained nuclear or cytoplasmic survivin were significantly different between cystitic and normal tissues but not between cystitic and TCC tissues. Four of 7 cystitic tissues were positive for survivin mRNA, which was comparable with results for TCC and normal tissues.
Nuclear survivin was detected in TCC and cystitic tissues but not in normal urinary bladder tissues. Additional studies are needed to determine whether nuclear survivin contributes to the development or progression of TCC.</abstract><cop>United States</cop><pmid>18672973</pmid><doi>10.2460/ajvr.69.8.1073</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | American journal of veterinary research, 2008-08, Vol.69 (8), p.1073-1078 |
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| subjects | animal proteins Animals apoptosis bladder bladder diseases carcinoma Carcinoma, Transitional Cell - metabolism Carcinoma, Transitional Cell - veterinary cell nucleus Cell Nucleus - metabolism cell proliferation cystitis Cystitis - metabolism Cystitis - veterinary cytoplasm dog diseases Dog Diseases - metabolism Dogs histology Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism neoplasms normal values pathogenesis Reference Values Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics survivin tissue analysis transitional cell carcinoma Urinary Bladder - metabolism Urinary Bladder Neoplasms - metabolism Urinary Bladder Neoplasms - veterinary |
| title | Comparison of distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma, or histologically normal urinary bladders |
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