Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?
We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2005-06, Vol.308 (5727), p.1469-1472 |
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| creator | Kural, Comert Kim, Hwajin Syed, Sheyum Goshima, Gohta Gelfand, Vladimir I Selvin, Paul R |
| description | We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be [approximately]8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed. |
| doi_str_mv | 10.1126/science.1108408 |
| format | Article |
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We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be [approximately]8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1108408</identifier><identifier>PMID: 15817813</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Accuracy ; Animals ; Average speed ; Biochemistry ; Biological and medical sciences ; Biological Transport ; cell biology ; Cell Line ; cell lines ; Cell organelles ; Cell structures and functions ; Cells ; contractile proteins ; Correlation ; Drosophila ; Drosophila melanogaster ; dynein ATPase ; Dyneins - physiology ; Fiona ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Green Fluorescent Proteins ; Human mechanics ; Inhibition ; Insects ; kinensin ; Kinesin ; Kinesin - physiology ; Methods ; Microtubules ; Miscellaneous ; Molecular and cellular biology ; Molecular Motor Proteins - physiology ; Motors ; Movement (Physiology) ; Organelles ; peroxisome movement ; Peroxisomes ; Peroxisomes - metabolism ; Proteins ; Speed ; Testing ; Velocity ; War</subject><ispartof>Science (American Association for the Advancement of Science), 2005-06, Vol.308 (5727), p.1469-1472</ispartof><rights>Copyright 2005 American Association for the Advancement of Science</rights><rights>2005 INIST-CNRS</rights><rights>COPYRIGHT 2005 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Jun 3, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c767t-a6692ef4495faf4b843c6fdfb4e89afbd0e88fe66302005687532fb29cfc0d673</citedby><cites>FETCH-LOGICAL-c767t-a6692ef4495faf4b843c6fdfb4e89afbd0e88fe66302005687532fb29cfc0d673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3841511$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3841511$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16834411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15817813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kural, Comert</creatorcontrib><creatorcontrib>Kim, Hwajin</creatorcontrib><creatorcontrib>Syed, Sheyum</creatorcontrib><creatorcontrib>Goshima, Gohta</creatorcontrib><creatorcontrib>Gelfand, Vladimir I</creatorcontrib><creatorcontrib>Selvin, Paul R</creatorcontrib><title>Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be [approximately]8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.</description><subject>Accuracy</subject><subject>Animals</subject><subject>Average speed</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>cell biology</subject><subject>Cell Line</subject><subject>cell lines</subject><subject>Cell organelles</subject><subject>Cell structures and functions</subject><subject>Cells</subject><subject>contractile proteins</subject><subject>Correlation</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>dynein ATPase</subject><subject>Dyneins - physiology</subject><subject>Fiona</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Green Fluorescent Proteins</subject><subject>Human mechanics</subject><subject>Inhibition</subject><subject>Insects</subject><subject>kinensin</subject><subject>Kinesin</subject><subject>Kinesin - physiology</subject><subject>Methods</subject><subject>Microtubules</subject><subject>Miscellaneous</subject><subject>Molecular and cellular biology</subject><subject>Molecular Motor Proteins - physiology</subject><subject>Motors</subject><subject>Movement (Physiology)</subject><subject>Organelles</subject><subject>peroxisome movement</subject><subject>Peroxisomes</subject><subject>Peroxisomes - metabolism</subject><subject>Proteins</subject><subject>Speed</subject><subject>Testing</subject><subject>Velocity</subject><subject>War</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN00Fv0zAUAOAIgdgonLkgiIYAcchmx47jcEGlQJkoFKnbOFqu8xylSuxhJ9P273HXiKmomqocnPh978WO86LoOUbHGKfsxKsajILwgDhF_EF0iFGRJUWKyMPoECHCEo7y7CB64v0KoRAryOPoAGcc5xyTw2jxvTbgaxNLU8afbwyE2x_2CmIZ_wJnr2tvW4jD5EV9ZT_E4_isrxKrk9_SxdbFE2tdWRvZQXmb1oLpPj6NHmnZeHg2jKPo_OuXs8m3ZDafnk7Gs0TlLO8SyViRgqa0yLTUdMkpUUyXekmBF1IvSwSca2CMoBShjPE8I6lepoXSCpUsJ6Po3abupbN_evCdaGuvoGmkAdt7kdOMEY4p3UOSlBYYreXbeyXLeUHzsKZRdPQfXNnembBfkWLCcFgsDuj1BlWyAVEbbTsn1bqiGGNCCM5Ivn5nskNVYMDJxhrQdZje8sc7fLhKaGu1M-H9VkIwHVx3ley9F6eLn_vb-cX-9tN0b8uns_s-yGCVbRqoQISfaDLf9icbr5z13oEWl65upbsRGIl1n4ihT8TQJyHj5XB6_bKF8s4PjRHAmwFIr2SjnTSq9neOcUIpXp_wi41b-c66f3HCKc5uw682YS2tkJULJc4XKcIEYYQw5oj8BbcFJKU</recordid><startdate>20050603</startdate><enddate>20050603</enddate><creator>Kural, Comert</creator><creator>Kim, Hwajin</creator><creator>Syed, Sheyum</creator><creator>Goshima, Gohta</creator><creator>Gelfand, Vladimir I</creator><creator>Selvin, Paul R</creator><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>0-V</scope><scope>3V.</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88B</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CJNVE</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9-</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0K</scope><scope>M0P</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEDU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050603</creationdate><title>Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?</title><author>Kural, Comert ; 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| subjects | Accuracy Animals Average speed Biochemistry Biological and medical sciences Biological Transport cell biology Cell Line cell lines Cell organelles Cell structures and functions Cells contractile proteins Correlation Drosophila Drosophila melanogaster dynein ATPase Dyneins - physiology Fiona Fluorescence Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins Human mechanics Inhibition Insects kinensin Kinesin Kinesin - physiology Methods Microtubules Miscellaneous Molecular and cellular biology Molecular Motor Proteins - physiology Motors Movement (Physiology) Organelles peroxisome movement Peroxisomes Peroxisomes - metabolism Proteins Speed Testing Velocity War |
| title | Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement? |
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