Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis
Ferredoxins are required to supply electrons to the cytochrome P450 enzymes involved in cross-linking reactions during the biosynthesis of the glycopeptide antibiotics balhimycin and vancomycin. However, the biosynthetic gene clusters for these antibiotics contain no ferredoxin- or ferredoxin reduct...
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| Veröffentlicht in: | FEMS microbiology letters 2010-05, Vol.306 (1), p.45-53 |
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| creator | Geib, Nina Weber, Tilmann Wörtz, Tanja Zerbe, Katja Wohlleben, Wolfgang Robinson, John A |
| description | Ferredoxins are required to supply electrons to the cytochrome P450 enzymes involved in cross-linking reactions during the biosynthesis of the glycopeptide antibiotics balhimycin and vancomycin. However, the biosynthetic gene clusters for these antibiotics contain no ferredoxin- or ferredoxin reductase-like genes. In a search for potential ferredoxin partners for these P450s, here, we report an in silico analysis of the draft genome sequence of the balhimycin producer Amycolatopsis balhimycina, which revealed 11 putative Fe-S-containing ferredoxin genes. We show that two members (balFd-V and balFd-VII), produced as native-like holo-[3Fe-4S] ferredoxins in Escherichia coli, could supply electrons to the P450 OxyB (CYP165B) from both A. balhimycina and the vancomycin producer Amycolatopsis orientalis, and support in vitro turnover of peptidyl carrier protein-bound peptide substrates into monocyclic cross-linked products. These results show that ferredoxins encoded in the antibiotic-producing strain can act in a degenerate manner in supporting the catalytic functions of glycopeptide biosynthetic P450 enzymes from the same as well as heterologous gene clusters. |
| doi_str_mv | 10.1111/j.1574-6968.2010.01933.x |
| format | Article |
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However, the biosynthetic gene clusters for these antibiotics contain no ferredoxin- or ferredoxin reductase-like genes. In a search for potential ferredoxin partners for these P450s, here, we report an in silico analysis of the draft genome sequence of the balhimycin producer Amycolatopsis balhimycina, which revealed 11 putative Fe-S-containing ferredoxin genes. We show that two members (balFd-V and balFd-VII), produced as native-like holo-[3Fe-4S] ferredoxins in Escherichia coli, could supply electrons to the P450 OxyB (CYP165B) from both A. balhimycina and the vancomycin producer Amycolatopsis orientalis, and support in vitro turnover of peptidyl carrier protein-bound peptide substrates into monocyclic cross-linked products. These results show that ferredoxins encoded in the antibiotic-producing strain can act in a degenerate manner in supporting the catalytic functions of glycopeptide biosynthetic P450 enzymes from the same as well as heterologous gene clusters.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2010.01933.x</identifier><identifier>PMID: 20337711</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Actinomycetales - enzymology ; Actinomycetales - genetics ; Amino Acid Sequence ; Amycolatopsis ; Anti-Bacterial Agents - biosynthesis ; Antibiotics ; balhimycin ; Biosynthesis ; Cloning, Molecular ; Computational Biology ; Crosslinking ; Cytochrome ; cytochrome P-450 ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Cytochromes P450 ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; E coli ; Electrons ; enzyme ; Enzymes ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Ferredoxin ; Ferredoxin reductase ; Ferredoxins - genetics ; Ferredoxins - metabolism ; Gene clusters ; Genes ; Genome, Bacterial ; Genomes ; Glycopeptides - biosynthesis ; metalloenzymes ; Microbiology ; Molecular Sequence Data ; Nucleotide sequence ; peptide ; Protein turnover ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Reductases ; Sequence Alignment ; Sequence Analysis, DNA ; Substrates ; Vancomycin ; Vancomycin - analogs & derivatives ; Vancomycin - biosynthesis</subject><ispartof>FEMS microbiology letters, 2010-05, Vol.306 (1), p.45-53</ispartof><rights>2010 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2010</rights><rights>2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2010 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4963-3d9c515637615655b9bad6ab60d4249cf0882c3a6c09b449483c22fa3c7fa0243</citedby><cites>FETCH-LOGICAL-c4963-3d9c515637615655b9bad6ab60d4249cf0882c3a6c09b449483c22fa3c7fa0243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2010.01933.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2010.01933.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20337711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geib, Nina</creatorcontrib><creatorcontrib>Weber, Tilmann</creatorcontrib><creatorcontrib>Wörtz, Tanja</creatorcontrib><creatorcontrib>Zerbe, Katja</creatorcontrib><creatorcontrib>Wohlleben, Wolfgang</creatorcontrib><creatorcontrib>Robinson, John A</creatorcontrib><title>Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Ferredoxins are required to supply electrons to the cytochrome P450 enzymes involved in cross-linking reactions during the biosynthesis of the glycopeptide antibiotics balhimycin and vancomycin. However, the biosynthetic gene clusters for these antibiotics contain no ferredoxin- or ferredoxin reductase-like genes. In a search for potential ferredoxin partners for these P450s, here, we report an in silico analysis of the draft genome sequence of the balhimycin producer Amycolatopsis balhimycina, which revealed 11 putative Fe-S-containing ferredoxin genes. We show that two members (balFd-V and balFd-VII), produced as native-like holo-[3Fe-4S] ferredoxins in Escherichia coli, could supply electrons to the P450 OxyB (CYP165B) from both A. balhimycina and the vancomycin producer Amycolatopsis orientalis, and support in vitro turnover of peptidyl carrier protein-bound peptide substrates into monocyclic cross-linked products. These results show that ferredoxins encoded in the antibiotic-producing strain can act in a degenerate manner in supporting the catalytic functions of glycopeptide biosynthetic P450 enzymes from the same as well as heterologous gene clusters.</description><subject>Actinomycetales - enzymology</subject><subject>Actinomycetales - genetics</subject><subject>Amino Acid Sequence</subject><subject>Amycolatopsis</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Antibiotics</subject><subject>balhimycin</subject><subject>Biosynthesis</subject><subject>Cloning, Molecular</subject><subject>Computational Biology</subject><subject>Crosslinking</subject><subject>Cytochrome</subject><subject>cytochrome P-450</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochrome P450</subject><subject>Cytochromes P450</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>E coli</subject><subject>Electrons</subject><subject>enzyme</subject><subject>Enzymes</subject><subject>Escherichia coli - 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enzymology</topic><topic>Actinomycetales - genetics</topic><topic>Amino Acid Sequence</topic><topic>Amycolatopsis</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Antibiotics</topic><topic>balhimycin</topic><topic>Biosynthesis</topic><topic>Cloning, Molecular</topic><topic>Computational Biology</topic><topic>Crosslinking</topic><topic>Cytochrome</topic><topic>cytochrome P-450</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Cytochrome P450</topic><topic>Cytochromes P450</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>E coli</topic><topic>Electrons</topic><topic>enzyme</topic><topic>Enzymes</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Ferredoxin</topic><topic>Ferredoxin reductase</topic><topic>Ferredoxins - genetics</topic><topic>Ferredoxins - metabolism</topic><topic>Gene clusters</topic><topic>Genes</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Glycopeptides - 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Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geib, Nina</au><au>Weber, Tilmann</au><au>Wörtz, Tanja</au><au>Zerbe, Katja</au><au>Wohlleben, Wolfgang</au><au>Robinson, John A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2010-05</date><risdate>2010</risdate><volume>306</volume><issue>1</issue><spage>45</spage><epage>53</epage><pages>45-53</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Ferredoxins are required to supply electrons to the cytochrome P450 enzymes involved in cross-linking reactions during the biosynthesis of the glycopeptide antibiotics balhimycin and vancomycin. However, the biosynthetic gene clusters for these antibiotics contain no ferredoxin- or ferredoxin reductase-like genes. In a search for potential ferredoxin partners for these P450s, here, we report an in silico analysis of the draft genome sequence of the balhimycin producer Amycolatopsis balhimycina, which revealed 11 putative Fe-S-containing ferredoxin genes. We show that two members (balFd-V and balFd-VII), produced as native-like holo-[3Fe-4S] ferredoxins in Escherichia coli, could supply electrons to the P450 OxyB (CYP165B) from both A. balhimycina and the vancomycin producer Amycolatopsis orientalis, and support in vitro turnover of peptidyl carrier protein-bound peptide substrates into monocyclic cross-linked products. These results show that ferredoxins encoded in the antibiotic-producing strain can act in a degenerate manner in supporting the catalytic functions of glycopeptide biosynthetic P450 enzymes from the same as well as heterologous gene clusters.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>20337711</pmid><doi>10.1111/j.1574-6968.2010.01933.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEMS microbiology letters, 2010-05, Vol.306 (1), p.45-53 |
| issn | 0378-1097 1574-6968 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Actinomycetales - enzymology Actinomycetales - genetics Amino Acid Sequence Amycolatopsis Anti-Bacterial Agents - biosynthesis Antibiotics balhimycin Biosynthesis Cloning, Molecular Computational Biology Crosslinking Cytochrome cytochrome P-450 Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Cytochromes P450 DNA, Bacterial - chemistry DNA, Bacterial - genetics E coli Electrons enzyme Enzymes Escherichia coli - genetics Escherichia coli - metabolism Ferredoxin Ferredoxin reductase Ferredoxins - genetics Ferredoxins - metabolism Gene clusters Genes Genome, Bacterial Genomes Glycopeptides - biosynthesis metalloenzymes Microbiology Molecular Sequence Data Nucleotide sequence peptide Protein turnover Recombinant Proteins - genetics Recombinant Proteins - metabolism Reductases Sequence Alignment Sequence Analysis, DNA Substrates Vancomycin Vancomycin - analogs & derivatives Vancomycin - biosynthesis |
| title | Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis |
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