Enzyme activities of isolated hepatic peroxisomes from genetically lean and obese male mice
Hepatic peroxisomes have been isolated on isopycnic sucrose gradients from white mice [HA(ICR)] and lean and obese (C57BL/6J) mice. Nearly all of the catalase activity was in the peroxisomal fraction. The activity for β-oxidation of palmitoyl-CoA was about threefold higher per milligram of protein i...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1979-03, Vol.193 (1), p.179-185 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Murphy, Patricia A. Krahling, Jeffrey B. Gee, Robert Kirk, James R. Tolbert, N.E. |
| description | Hepatic peroxisomes have been isolated on isopycnic sucrose gradients from white mice [HA(ICR)] and lean and obese (C57BL/6J) mice. Nearly all of the catalase activity was in the peroxisomal fraction. The activity for β-oxidation of palmitoyl-CoA was about threefold higher per milligram of protein in the isolated peroxisomal fraction or per gram of liver from the obese mouse compared to its lean littermates. Glycerol-3-phosphate dehydrogenase activity also was higher in the peroxisomes and cytoplasm of the obese mouse. The matrix enzymes of the organelles, catalase and urate oxidase of the peroxisome and glutamate dehydrogenase of the mitochondria, had similar activities per gram of liver from either lean or obese mice. Membrane components, NADPH: cytochrome
c reductase of the microsomes and β-hydroxybutyrate dehydrogenase of the mitochondria, had lower activities in the obese mouse in inverse proportion to the larger size of the liver. |
| doi_str_mv | 10.1016/0003-9861(79)90021-3 |
| format | Article |
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c reductase of the microsomes and β-hydroxybutyrate dehydrogenase of the mitochondria, had lower activities in the obese mouse in inverse proportion to the larger size of the liver.</description><subject>Animals</subject><subject>Body Weight</subject><subject>Glycerolphosphate Dehydrogenase - metabolism</subject><subject>In Vitro Techniques</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Obese</subject><subject>Microbodies - enzymology</subject><subject>NADH, NADPH Oxidoreductases - metabolism</subject><subject>Obesity - enzymology</subject><subject>Organoids - enzymology</subject><subject>Palmitoyl Coenzyme A</subject><subject>Species Specificity</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQx4P4Wle_gYecRA_VSZM07UUQ8QWCFz15CGky1UjbrElXXD-9XXfZo5cZmP8D5kfIMYNzBqy4AACeVWXBTlV1VgHkLONbZMKgKjLgpdgmk41lnxyk9AHAmCjyPbIr5GhQE_J60_8sOqTGDv7LDx4TDQ31KbRmQEffcWYGb-kMY_ger92oNzF09A17HAXTtgvaoump6R0NNSaknWnH4S0ekp3GtAmP1ntKXm5vnq_vs8enu4frq8fMijwfsrLIK6tELYFb1UDeSMSalxYVgHGyrhpe2dwqWddKYq6aouRCKmaUcc6VwKfkZNU7i-FzjmnQnU8W29b0GOZJKyGlYGxpFCujjSGliI2eRd-ZuNAM9BKpXvLSS15aVfoPqeZj7HjdP687dJvQiuEoX65kHH_88hh1sh57i85HtIN2wf_f_wuUi4Yp</recordid><startdate>197903</startdate><enddate>197903</enddate><creator>Murphy, Patricia A.</creator><creator>Krahling, Jeffrey B.</creator><creator>Gee, Robert</creator><creator>Kirk, James R.</creator><creator>Tolbert, N.E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197903</creationdate><title>Enzyme activities of isolated hepatic peroxisomes from genetically lean and obese male mice</title><author>Murphy, Patricia A. ; Krahling, Jeffrey B. ; Gee, Robert ; Kirk, James R. ; Tolbert, N.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-8629c74b503c7f02f5eeb38ce700ad5b9f39c2c75bb75e27f6834571a7addd803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Body Weight</topic><topic>Glycerolphosphate Dehydrogenase - metabolism</topic><topic>In Vitro Techniques</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Obese</topic><topic>Microbodies - enzymology</topic><topic>NADH, NADPH Oxidoreductases - metabolism</topic><topic>Obesity - enzymology</topic><topic>Organoids - enzymology</topic><topic>Palmitoyl Coenzyme A</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murphy, Patricia A.</creatorcontrib><creatorcontrib>Krahling, Jeffrey B.</creatorcontrib><creatorcontrib>Gee, Robert</creatorcontrib><creatorcontrib>Kirk, James R.</creatorcontrib><creatorcontrib>Tolbert, N.E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murphy, Patricia A.</au><au>Krahling, Jeffrey B.</au><au>Gee, Robert</au><au>Kirk, James R.</au><au>Tolbert, N.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme activities of isolated hepatic peroxisomes from genetically lean and obese male mice</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1979-03</date><risdate>1979</risdate><volume>193</volume><issue>1</issue><spage>179</spage><epage>185</epage><pages>179-185</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Hepatic peroxisomes have been isolated on isopycnic sucrose gradients from white mice [HA(ICR)] and lean and obese (C57BL/6J) mice. Nearly all of the catalase activity was in the peroxisomal fraction. The activity for β-oxidation of palmitoyl-CoA was about threefold higher per milligram of protein in the isolated peroxisomal fraction or per gram of liver from the obese mouse compared to its lean littermates. Glycerol-3-phosphate dehydrogenase activity also was higher in the peroxisomes and cytoplasm of the obese mouse. The matrix enzymes of the organelles, catalase and urate oxidase of the peroxisome and glutamate dehydrogenase of the mitochondria, had similar activities per gram of liver from either lean or obese mice. Membrane components, NADPH: cytochrome
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| ispartof | Archives of biochemistry and biophysics, 1979-03, Vol.193 (1), p.179-185 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Body Weight Glycerolphosphate Dehydrogenase - metabolism In Vitro Techniques Liver - enzymology Male Mice Mice, Obese Microbodies - enzymology NADH, NADPH Oxidoreductases - metabolism Obesity - enzymology Organoids - enzymology Palmitoyl Coenzyme A Species Specificity |
| title | Enzyme activities of isolated hepatic peroxisomes from genetically lean and obese male mice |
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