Evaluation of a modified dye pour-plate auxanographic method for the rapid identification of clinically significant yeasts. Comparison with two commercial systems
A modified dye pour-plate auxanographic (DPPA) method for the presumptive identification of medically important yeasts was evaluated, in a comparative study with a conventional procedure, the API 20C clinical yeast system (Analytab Products Inc.), and the Uni-Yeast-Tek (UYT) system. The 174 coded cl...
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| Veröffentlicht in: | Medical microbiology and immunology 1979-03, Vol.167 (1), p.11-20 |
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| container_title | Medical microbiology and immunology |
| container_volume | 167 |
| creator | Weymann, L H Stager, C E Qadri, S G Villarreal, A Qadri, S M |
| description | A modified dye pour-plate auxanographic (DPPA) method for the presumptive identification of medically important yeasts was evaluated, in a comparative study with a conventional procedure, the API 20C clinical yeast system (Analytab Products Inc.), and the Uni-Yeast-Tek (UYT) system. The 174 coded clinical isolates were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The identification accuracies with DPPA, API, and UYT were 95%, 93%, and 99% respectively. DPPA and API required more time to inoculate but gave rapid identification profiles. UYT was simple to inoculate and both UYT and DPPA were easy to read. Cost analysis of the three rapid methods demonstrated DPPA to be the most economical making it a feasible alternative for small clinical laboratories as well as large laboratories possessing the facilities to make their own media. |
| doi_str_mv | 10.1007/BF02123291 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0300-8584 |
| ispartof | Medical microbiology and immunology, 1979-03, Vol.167 (1), p.11-20 |
| issn | 0300-8584 1432-1831 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Carbohydrate Metabolism Fermentation Humans Microbiological Techniques Mycoses - microbiology Yeasts - classification Yeasts - metabolism |
| title | Evaluation of a modified dye pour-plate auxanographic method for the rapid identification of clinically significant yeasts. Comparison with two commercial systems |
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