Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes
In a patient with an IgG lambda monoclonal serum component possessing anti-streptolysin O activity, we have demonstrated peripheral blood B and T lymphocytes with shared or similar idiotypes. The idiotypic T lymphocyte membrane structure was capable of binding the specific antigen (SLO). After radio...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 1979-06, Vol.122 (6), p.2413-2417 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2417 |
|---|---|
| container_issue | 6 |
| container_start_page | 2413 |
| container_title | The Journal of immunology (1950) |
| container_volume | 122 |
| creator | Lea, Tor Forre, oystein T Michaelsen, Terje E Natvig, Jacob B |
| description | In a patient with an IgG lambda monoclonal serum component possessing anti-streptolysin O activity, we have demonstrated peripheral blood B and T lymphocytes with shared or similar idiotypes. The idiotypic T lymphocyte membrane structure was capable of binding the specific antigen (SLO). After radioiodination and subsequent detergent solubilization of the same T cell population, immunoprecipitation of the lysate by employing anti-idiotypic antibodies, resulted in the isolation of a polypeptide chain with a m.w. of 70,000 on SDS polyacrylamide gels under reducing conditions. The polypeptide expressed no isotypic immunoglobulin markers. Internal labeling experiments indicated that this membrane structure was actively synthesized by the T lymphocytes. |
| doi_str_mv | 10.4049/jimmunol.122.6.2413 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74549152</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>74549152</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2913-c8b5589b3cc2bcea045c62b56c45fb96172a8d272bd06318bef15c6e8ea160793</originalsourceid><addsrcrecordid>eNpNkM9PgzAYhhvjrzn9C_TQk57AtrQFLiZuUbdkiSbOc1NKEQyl2I4Q_ntZmMbTl3zv876HB4BrjEKKaHr_VRnTNbYOMSEhDwnF0RGYYcZQwDnix2CGECEBjnl8Di68_0IIcUToGTiNxl9EZ-DhvZRO53CdV3Y3tNpD28BVZ2QD37Sr2lI7WcNFbW0OF1A2OdzCzWDa0qphp_0lOClk7fXV4c7Bx_PTdrkKNq8v6-XjJlAkxVGgkoyxJM0ipUimtESUKU4yxhVlRZZyHBOZ5CQmWY54hJNMF3gkdKIl5ihOozm4nXZbZ7877XfCVF7pupaNtp0XMWU0xYyMYDSBylnvnS5E6yoj3SAwEntp4leaGKUJLvbSxtbNYb7LjM7_OpOlMb6b4rL6LPvKaeGNrOsRxqLv-39DP1vIdpM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74549152</pqid></control><display><type>article</type><title>Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Lea, Tor ; Forre, oystein T ; Michaelsen, Terje E ; Natvig, Jacob B</creator><creatorcontrib>Lea, Tor ; Forre, oystein T ; Michaelsen, Terje E ; Natvig, Jacob B</creatorcontrib><description>In a patient with an IgG lambda monoclonal serum component possessing anti-streptolysin O activity, we have demonstrated peripheral blood B and T lymphocytes with shared or similar idiotypes. The idiotypic T lymphocyte membrane structure was capable of binding the specific antigen (SLO). After radioiodination and subsequent detergent solubilization of the same T cell population, immunoprecipitation of the lysate by employing anti-idiotypic antibodies, resulted in the isolation of a polypeptide chain with a m.w. of 70,000 on SDS polyacrylamide gels under reducing conditions. The polypeptide expressed no isotypic immunoglobulin markers. Internal labeling experiments indicated that this membrane structure was actively synthesized by the T lymphocytes.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.122.6.2413</identifier><identifier>PMID: 376734</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Antigens ; B-Lymphocytes - immunology ; Classification ; Electrophoresis, Polyacrylamide Gel ; Fluorescent Antibody Technique ; Humans ; Immunoglobulin Idiotypes ; Lymphocytes ; Membrane Proteins - isolation & purification ; T-Lymphocytes - immunology ; T-Lymphocytes - metabolism</subject><ispartof>The Journal of immunology (1950), 1979-06, Vol.122 (6), p.2413-2417</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2913-c8b5589b3cc2bcea045c62b56c45fb96172a8d272bd06318bef15c6e8ea160793</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/376734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lea, Tor</creatorcontrib><creatorcontrib>Forre, oystein T</creatorcontrib><creatorcontrib>Michaelsen, Terje E</creatorcontrib><creatorcontrib>Natvig, Jacob B</creatorcontrib><title>Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>In a patient with an IgG lambda monoclonal serum component possessing anti-streptolysin O activity, we have demonstrated peripheral blood B and T lymphocytes with shared or similar idiotypes. The idiotypic T lymphocyte membrane structure was capable of binding the specific antigen (SLO). After radioiodination and subsequent detergent solubilization of the same T cell population, immunoprecipitation of the lysate by employing anti-idiotypic antibodies, resulted in the isolation of a polypeptide chain with a m.w. of 70,000 on SDS polyacrylamide gels under reducing conditions. The polypeptide expressed no isotypic immunoglobulin markers. Internal labeling experiments indicated that this membrane structure was actively synthesized by the T lymphocytes.</description><subject>Antigens</subject><subject>B-Lymphocytes - immunology</subject><subject>Classification</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Immunoglobulin Idiotypes</subject><subject>Lymphocytes</subject><subject>Membrane Proteins - isolation & purification</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - metabolism</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM9PgzAYhhvjrzn9C_TQk57AtrQFLiZuUbdkiSbOc1NKEQyl2I4Q_ntZmMbTl3zv876HB4BrjEKKaHr_VRnTNbYOMSEhDwnF0RGYYcZQwDnix2CGECEBjnl8Di68_0IIcUToGTiNxl9EZ-DhvZRO53CdV3Y3tNpD28BVZ2QD37Sr2lI7WcNFbW0OF1A2OdzCzWDa0qphp_0lOClk7fXV4c7Bx_PTdrkKNq8v6-XjJlAkxVGgkoyxJM0ipUimtESUKU4yxhVlRZZyHBOZ5CQmWY54hJNMF3gkdKIl5ihOozm4nXZbZ7877XfCVF7pupaNtp0XMWU0xYyMYDSBylnvnS5E6yoj3SAwEntp4leaGKUJLvbSxtbNYb7LjM7_OpOlMb6b4rL6LPvKaeGNrOsRxqLv-39DP1vIdpM</recordid><startdate>197906</startdate><enddate>197906</enddate><creator>Lea, Tor</creator><creator>Forre, oystein T</creator><creator>Michaelsen, Terje E</creator><creator>Natvig, Jacob B</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197906</creationdate><title>Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes</title><author>Lea, Tor ; Forre, oystein T ; Michaelsen, Terje E ; Natvig, Jacob B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2913-c8b5589b3cc2bcea045c62b56c45fb96172a8d272bd06318bef15c6e8ea160793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Antigens</topic><topic>B-Lymphocytes - immunology</topic><topic>Classification</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Immunoglobulin Idiotypes</topic><topic>Lymphocytes</topic><topic>Membrane Proteins - isolation & purification</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lea, Tor</creatorcontrib><creatorcontrib>Forre, oystein T</creatorcontrib><creatorcontrib>Michaelsen, Terje E</creatorcontrib><creatorcontrib>Natvig, Jacob B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lea, Tor</au><au>Forre, oystein T</au><au>Michaelsen, Terje E</au><au>Natvig, Jacob B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1979-06</date><risdate>1979</risdate><volume>122</volume><issue>6</issue><spage>2413</spage><epage>2417</epage><pages>2413-2417</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>In a patient with an IgG lambda monoclonal serum component possessing anti-streptolysin O activity, we have demonstrated peripheral blood B and T lymphocytes with shared or similar idiotypes. The idiotypic T lymphocyte membrane structure was capable of binding the specific antigen (SLO). After radioiodination and subsequent detergent solubilization of the same T cell population, immunoprecipitation of the lysate by employing anti-idiotypic antibodies, resulted in the isolation of a polypeptide chain with a m.w. of 70,000 on SDS polyacrylamide gels under reducing conditions. The polypeptide expressed no isotypic immunoglobulin markers. Internal labeling experiments indicated that this membrane structure was actively synthesized by the T lymphocytes.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>376734</pmid><doi>10.4049/jimmunol.122.6.2413</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1979-06, Vol.122 (6), p.2413-2417 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74549152 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antigens B-Lymphocytes - immunology Classification Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Humans Immunoglobulin Idiotypes Lymphocytes Membrane Proteins - isolation & purification T-Lymphocytes - immunology T-Lymphocytes - metabolism |
| title | Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T09%3A10%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Shared%20Idiotypes%20on%20Human%20Peripheral%20Blood%20B%20and%20T%20Lymphocytes&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Lea,%20Tor&rft.date=1979-06&rft.volume=122&rft.issue=6&rft.spage=2413&rft.epage=2417&rft.pages=2413-2417&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.122.6.2413&rft_dat=%3Cproquest_cross%3E74549152%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74549152&rft_id=info:pmid/376734&rfr_iscdi=true |