DNA-dependent RNA polymerases in skeletal muscle cells differentiating in vitro
The main classes of RNA polymerase in cultured chick embryo skeletal muscle cells were identified and their levels determined during differentiation in vitro. Cell cultures contained a high proportion of muscle cells (>80%) and exhibited a high degree of fusion (>80%). The levels of RNA polyme...
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| Veröffentlicht in: | Developmental biology 1979, Vol.68 (1), p.280-286 |
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| container_title | Developmental biology |
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| creator | van der Westhuyzen, D.R. |
| description | The main classes of RNA polymerase in cultured chick embryo skeletal muscle cells were identified and their levels determined during differentiation
in vitro. Cell cultures contained a high proportion of muscle cells (>80%) and exhibited a high degree of fusion (>80%). The levels of RNA polymerases I and II increased by 1.8 and 1.5 times, respectively, from prefusion myoblasts (24 hr) to postfusion myotubes (120 hr). RNA polymerase III, a single peak on DEAE-Sephadex chromatography, was less than 7% of the total activity. The results suggest that the rates of synthesis of the main RNA types, which reportedly decline during myogenesis, are not determined by the relative levels of the main RNA polymerases and are more likely a reflection of chromatin template availability. |
| doi_str_mv | 10.1016/0012-1606(79)90259-8 |
| format | Article |
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| ispartof | Developmental biology, 1979, Vol.68 (1), p.280-286 |
| issn | 0012-1606 1095-564X |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | animal science Animals Cell Differentiation Cells, Cultured Chick Embryo - enzymology Chromatography, Ion Exchange DNA-Directed RNA Polymerases - metabolism livestock Muscles - enzymology RNA Polymerase I - metabolism RNA Polymerase II - metabolism RNA Polymerase III - metabolism zoology |
| title | DNA-dependent RNA polymerases in skeletal muscle cells differentiating in vitro |
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