C1q: Isolation from Human Serum in High Yield by Affinity Chromatography and Development of a Highly Sensitive Hemolytic Assay

C1q, a subcomponent of the first component of complement, has been isolated from human serum in fully hemolytically active form by affinity column chromatography and gel filtration with Bio-Gel A-5M. The affinity column was prepared by covalent coupling of purified human IgG to CNBr-activated Sephar...

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Veröffentlicht in:	The Journal of immunology (1950) 1979-05, Vol.122 (5), p.2103-2111
Hauptverfasser:	Kolb, William P, Kolb, Linda M, Podack, Eckhard R
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity Complement C1 - immunology Complement C1 - isolation & purification Complement C1 Inactivator Proteins - immunology Dose-Response Relationship, Immunologic Electrophoresis, Polyacrylamide Gel Hemolysis Humans Immunodiffusion Immunoelectrophoresis Immunologic Techniques
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