Glial fibrillary acidic protein (GFAP): Purification from human fibrillary astrocytoma, development and validation of a radioimmunoassay for GFAP-like immunoactivity
The extraction and purification of glial fibrillary acidic protein (GFAP) from human fibrillary cerebellar astrocytoma is described. Using an immunoperoxidase method, antisera raised to the protein showed specific staining of astrocytes in normal spinal cord and in tumours of astrocytic origin. A do...
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| Veröffentlicht in: | Journal of the neurological sciences 1979-01, Vol.41 (1), p.101-113 |
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| container_title | Journal of the neurological sciences |
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| creator | Palfreyman, J.W. Thomas, D.G.T. Ratcliffe, J.G. Graham, D.I. |
| description | The extraction and purification of glial fibrillary acidic protein (GFAP) from human fibrillary cerebellar astrocytoma is described. Using an immunoperoxidase method, antisera raised to the protein showed specific staining of astrocytes in normal spinal cord and in tumours of astrocytic origin. A double antibody radioimmunoassay for GFAP in tissue extract was developed, the detection limit of the assay being 360 pg. Extracts of tissues other than brain or spinal cord did not cross-react significantly in the assay, neither did purified preparations of myelin basic and S-100 proteins. Levels of GFAP in normal CNS tissue were highest in spinal cord (1370 μg/g wet weight) but a level of 3050 μg/g wet weight was detected in a fibrillary astrocytoma. |
| doi_str_mv | 10.1016/0022-510X(79)90144-8 |
| format | Article |
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Using an immunoperoxidase method, antisera raised to the protein showed specific staining of astrocytes in normal spinal cord and in tumours of astrocytic origin. A double antibody radioimmunoassay for GFAP in tissue extract was developed, the detection limit of the assay being 360 pg. Extracts of tissues other than brain or spinal cord did not cross-react significantly in the assay, neither did purified preparations of myelin basic and S-100 proteins. Levels of GFAP in normal CNS tissue were highest in spinal cord (1370 μg/g wet weight) but a level of 3050 μg/g wet weight was detected in a fibrillary astrocytoma.</description><identifier>ISSN: 0022-510X</identifier><identifier>EISSN: 1878-5883</identifier><identifier>DOI: 10.1016/0022-510X(79)90144-8</identifier><identifier>PMID: 438840</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Astrocytes - analysis ; Astrocytes - ultrastructure ; Astrocytoma - analysis ; Astrocytoma - ultrastructure ; Cerebellar Neoplasms - analysis ; Cerebellar Neoplasms - ultrastructure ; Humans ; Molecular Weight ; Neoplasm Metastasis ; Nerve Tissue Proteins - isolation & purification ; Radioimmunoassay - methods ; Spinal Cord - ultrastructure</subject><ispartof>Journal of the neurological sciences, 1979-01, Vol.41 (1), p.101-113</ispartof><rights>1979</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0022510X79901448$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/438840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palfreyman, J.W.</creatorcontrib><creatorcontrib>Thomas, D.G.T.</creatorcontrib><creatorcontrib>Ratcliffe, J.G.</creatorcontrib><creatorcontrib>Graham, D.I.</creatorcontrib><title>Glial fibrillary acidic protein (GFAP): Purification from human fibrillary astrocytoma, development and validation of a radioimmunoassay for GFAP-like immunoactivity</title><title>Journal of the neurological sciences</title><addtitle>J Neurol Sci</addtitle><description>The extraction and purification of glial fibrillary acidic protein (GFAP) from human fibrillary cerebellar astrocytoma is described. Using an immunoperoxidase method, antisera raised to the protein showed specific staining of astrocytes in normal spinal cord and in tumours of astrocytic origin. A double antibody radioimmunoassay for GFAP in tissue extract was developed, the detection limit of the assay being 360 pg. Extracts of tissues other than brain or spinal cord did not cross-react significantly in the assay, neither did purified preparations of myelin basic and S-100 proteins. Levels of GFAP in normal CNS tissue were highest in spinal cord (1370 μg/g wet weight) but a level of 3050 μg/g wet weight was detected in a fibrillary astrocytoma.</description><subject>Astrocytes - analysis</subject><subject>Astrocytes - ultrastructure</subject><subject>Astrocytoma - analysis</subject><subject>Astrocytoma - ultrastructure</subject><subject>Cerebellar Neoplasms - analysis</subject><subject>Cerebellar Neoplasms - ultrastructure</subject><subject>Humans</subject><subject>Molecular Weight</subject><subject>Neoplasm Metastasis</subject><subject>Nerve Tissue Proteins - isolation & purification</subject><subject>Radioimmunoassay - methods</subject><subject>Spinal Cord - ultrastructure</subject><issn>0022-510X</issn><issn>1878-5883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUctuFDEQtBABlsAf5OATSqRM6LHn4ckBKYrIEilScgCJm-WnaBiPF9uz0n4Q_8lsdoXEqQ9V1d1VRchZDVc11N1HAMaqtobv5_1wMUDdNJV4QVa16EXVCsFfktU_yhvyNuefANAJMbwmrxouRAMr8mc9ohqpR51wHFXaUWXQoqGbFIvDiZ6v726eLq7p05zQo1EF40R9ioH-mIOa_lPmkqLZlRjUJbVu68a4CW4qVE2WbtWI9qCOniqalMWIIcxTVDmrHfUx0f2tasRfjh4RU3CLZfeOnHg1Zvf-OE_Jt7vPX2-_VA-P6_vbm4fKsQ5KxQcOTPQtCOUVMxqcY7oGrRkXHixnuofWm34QotOi81ox1eq26zX4XlvFT8mHw97F_e_Z5SIDZuMWe5OLc5Z90_KB1Xwhnh2Jsw7Oyk3CsEQgD7Eu8KcD7JZnt-iSzAbdZJzF5EyRNqKsQe5blPuK5L4i2Q_yuUUp-F-LHpI-</recordid><startdate>19790101</startdate><enddate>19790101</enddate><creator>Palfreyman, J.W.</creator><creator>Thomas, D.G.T.</creator><creator>Ratcliffe, J.G.</creator><creator>Graham, D.I.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19790101</creationdate><title>Glial fibrillary acidic protein (GFAP): Purification from human fibrillary astrocytoma, development and validation of a radioimmunoassay for GFAP-like immunoactivity</title><author>Palfreyman, J.W. ; Thomas, D.G.T. ; Ratcliffe, J.G. ; Graham, D.I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e260t-3930287508afa2cb0ee2b10bb238f0d32b705fc79886b86fba2a5b567b0f7bda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Astrocytes - analysis</topic><topic>Astrocytes - ultrastructure</topic><topic>Astrocytoma - analysis</topic><topic>Astrocytoma - ultrastructure</topic><topic>Cerebellar Neoplasms - analysis</topic><topic>Cerebellar Neoplasms - ultrastructure</topic><topic>Humans</topic><topic>Molecular Weight</topic><topic>Neoplasm Metastasis</topic><topic>Nerve Tissue Proteins - isolation & purification</topic><topic>Radioimmunoassay - methods</topic><topic>Spinal Cord - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palfreyman, J.W.</creatorcontrib><creatorcontrib>Thomas, D.G.T.</creatorcontrib><creatorcontrib>Ratcliffe, J.G.</creatorcontrib><creatorcontrib>Graham, D.I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the neurological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palfreyman, J.W.</au><au>Thomas, D.G.T.</au><au>Ratcliffe, J.G.</au><au>Graham, D.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glial fibrillary acidic protein (GFAP): Purification from human fibrillary astrocytoma, development and validation of a radioimmunoassay for GFAP-like immunoactivity</atitle><jtitle>Journal of the neurological sciences</jtitle><addtitle>J Neurol Sci</addtitle><date>1979-01-01</date><risdate>1979</risdate><volume>41</volume><issue>1</issue><spage>101</spage><epage>113</epage><pages>101-113</pages><issn>0022-510X</issn><eissn>1878-5883</eissn><abstract>The extraction and purification of glial fibrillary acidic protein (GFAP) from human fibrillary cerebellar astrocytoma is described. Using an immunoperoxidase method, antisera raised to the protein showed specific staining of astrocytes in normal spinal cord and in tumours of astrocytic origin. A double antibody radioimmunoassay for GFAP in tissue extract was developed, the detection limit of the assay being 360 pg. Extracts of tissues other than brain or spinal cord did not cross-react significantly in the assay, neither did purified preparations of myelin basic and S-100 proteins. Levels of GFAP in normal CNS tissue were highest in spinal cord (1370 μg/g wet weight) but a level of 3050 μg/g wet weight was detected in a fibrillary astrocytoma.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>438840</pmid><doi>10.1016/0022-510X(79)90144-8</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-510X |
| ispartof | Journal of the neurological sciences, 1979-01, Vol.41 (1), p.101-113 |
| issn | 0022-510X 1878-5883 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Astrocytes - analysis Astrocytes - ultrastructure Astrocytoma - analysis Astrocytoma - ultrastructure Cerebellar Neoplasms - analysis Cerebellar Neoplasms - ultrastructure Humans Molecular Weight Neoplasm Metastasis Nerve Tissue Proteins - isolation & purification Radioimmunoassay - methods Spinal Cord - ultrastructure |
| title | Glial fibrillary acidic protein (GFAP): Purification from human fibrillary astrocytoma, development and validation of a radioimmunoassay for GFAP-like immunoactivity |
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