A fluorimetric method for continuously assaying ATPase: Application to small specimens of glycerol-extracted muscle fibers
A continuous fluorimetric method using auxiliary-coupling enzymes such as pyruvate kinase and lactate dehydrogenase for measuring ADP production to assay ATPase activity is described. This method is simpler, more rapid, and more sensitive than the previously used spectrophotometric method. The appli...
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| Veröffentlicht in: | Analytical biochemistry 1979-01, Vol.92 (2), p.375-382 |
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| container_title | Analytical biochemistry |
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| creator | Takashi, Reiji Putnam, Susan |
| description | A continuous fluorimetric method using auxiliary-coupling enzymes such as pyruvate kinase and lactate dehydrogenase for measuring ADP production to assay ATPase activity is described. This method is simpler, more rapid, and more sensitive than the previously used spectrophotometric method. The application of this method for studying the ATPase of rabbit psoas muscle fibers during Mg
2+-ATP activation is also illustrated and discussed. |
| doi_str_mv | 10.1016/0003-2697(79)90674-2 |
| format | Article |
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| ispartof | Analytical biochemistry, 1979-01, Vol.92 (2), p.375-382 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74539177 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenosine Triphosphatases - analysis Animals Autoanalysis Enzyme Activation Glycerol L-Lactate Dehydrogenase - pharmacology Muscle Proteins - analysis Muscles - enzymology NAD - analysis Pyruvate Kinase - pharmacology Rabbits Spectrometry, Fluorescence |
| title | A fluorimetric method for continuously assaying ATPase: Application to small specimens of glycerol-extracted muscle fibers |
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