Solubilization of Antigens of Fasciola hepatica Which React with Antibodies to Schistosoma mansoni

Extracts of Fasciola hepatica adult worms contain antigens reactive with antisera prepared against Schistosoma mansoni adult worms. These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Sol...

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Veröffentlicht in:	The Journal of parasitology 1979-02, Vol.65 (1), p.55-60
Hauptverfasser:	Hillyer, George V., Pelley, Ronald P., Ana del Llano de Diaz
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Antibodies Antibodies - immunology Antibodies - isolation & purification Antigen-Antibody Reactions Antigens Antigens - isolation & purification Antiserum Centrifugation Chromatography, Gel Fasciola hepatica - immunology Homogenization Immunodiffusion Low speed Mice Reactivity Schistosoma mansoni - immunology Schistosomiasis - immunology Solubility Solubilization
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container_title	The Journal of parasitology
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creator	Hillyer, George V. Pelley, Ronald P. Ana del Llano de Diaz
description	Extracts of Fasciola hepatica adult worms contain antigens reactive with antisera prepared against Schistosoma mansoni adult worms. These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Solubilization is improved greatly by the addition of sodium dodecyl sulfate (0.03%) to the PBS. When this is done, one obtains approximately the same total amount of crude Lowry reactive material as with PBS extraction followed by high speed centrifugation but antigenic reactivity to an anti-S. mansoni antiserum increases enormously. The antigens liberated from F. hepatica SDS extraction are largely materials under 200,000 MW and over 60,000 MW.
doi_str_mv	10.2307/3280202
format	Article
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These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Solubilization is improved greatly by the addition of sodium dodecyl sulfate (0.03%) to the PBS. When this is done, one obtains approximately the same total amount of crude Lowry reactive material as with PBS extraction followed by high speed centrifugation but antigenic reactivity to an anti-S. mansoni antiserum increases enormously. The antigens liberated from F. hepatica SDS extraction are largely materials under 200,000 MW and over 60,000 MW.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3280202</identifier><identifier>PMID: 448600</identifier><language>eng</language><publisher>United States: American Society of Parasitologists</publisher><subject>Amino acids ; Animals ; Antibodies ; Antibodies - immunology ; Antibodies - isolation & purification ; Antigen-Antibody Reactions ; Antigens ; Antigens - isolation & purification ; Antiserum ; Centrifugation ; Chromatography, Gel ; Fasciola hepatica - immunology ; Homogenization ; Immunodiffusion ; Low speed ; Mice ; Reactivity ; Schistosoma mansoni - immunology ; Schistosomiasis - immunology ; Solubility ; Solubilization</subject><ispartof>The Journal of parasitology, 1979-02, Vol.65 (1), p.55-60</ispartof><rights>Copyright 1979 American Society of Parasitologists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c304t-bf378c908889a635eb37460ee68f79eac969d907d878a32f396ed8ae3285ef9c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3280202$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3280202$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/448600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hillyer, George V.</creatorcontrib><creatorcontrib>Pelley, Ronald P.</creatorcontrib><creatorcontrib>Ana del Llano de Diaz</creatorcontrib><title>Solubilization of Antigens of Fasciola hepatica Which React with Antibodies to Schistosoma mansoni</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Extracts of Fasciola hepatica adult worms contain antigens reactive with antisera prepared against Schistosoma mansoni adult worms. These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Solubilization is improved greatly by the addition of sodium dodecyl sulfate (0.03%) to the PBS. When this is done, one obtains approximately the same total amount of crude Lowry reactive material as with PBS extraction followed by high speed centrifugation but antigenic reactivity to an anti-S. mansoni antiserum increases enormously. 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These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Solubilization is improved greatly by the addition of sodium dodecyl sulfate (0.03%) to the PBS. When this is done, one obtains approximately the same total amount of crude Lowry reactive material as with PBS extraction followed by high speed centrifugation but antigenic reactivity to an anti-S. mansoni antiserum increases enormously. The antigens liberated from F. hepatica SDS extraction are largely materials under 200,000 MW and over 60,000 MW.</abstract><cop>United States</cop><pub>American Society of Parasitologists</pub><pmid>448600</pmid><doi>10.2307/3280202</doi><tpages>6</tpages></addata></record>
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subjects	Amino acids Animals Antibodies Antibodies - immunology Antibodies - isolation & purification Antigen-Antibody Reactions Antigens Antigens - isolation & purification Antiserum Centrifugation Chromatography, Gel Fasciola hepatica - immunology Homogenization Immunodiffusion Low speed Mice Reactivity Schistosoma mansoni - immunology Schistosomiasis - immunology Solubility Solubilization
title	Solubilization of Antigens of Fasciola hepatica Which React with Antibodies to Schistosoma mansoni
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