The Role of Monocytes in Phagocytosis and Mixed Leukocyte Reactivity in Human Acute Myeloid Leukemia
Lymphocyte proliferation, as measured by incorporation of tritiated thymidine (3H-TdR), was significantly enhanced (p < 0.01) when macrophages sensitized by target myeloblasts were added to monocyte-depleted lymphocyte fractions in the mixed leukocyte reaction (MLR) with human leukemic myeloblast...
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| Veröffentlicht in: | Immunological communications 1979, Vol.8 (3), p.279-301 |
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| container_title | Immunological communications |
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| creator | Miale, Thomas D. Stenke, Leif Å. L. Penchansky, Marta Lehtinen, Toini Reizenstein, Peter G. |
| description | Lymphocyte proliferation, as measured by incorporation of tritiated thymidine (3H-TdR), was significantly enhanced (p < 0.01) when macrophages sensitized by target myeloblasts were added to monocyte-depleted lymphocyte fractions in the mixed leukocyte reaction (MLR) with human leukemic myeloblasts as stimulators and panels of normal lymphocytes as responders. Monocyte addition in the same concentration range to unfractionated lymphocytes resulted in highly significant facilitation (p < 0.0001) of MLR response patterns to myeloblastic stimulation. However, with substitution of a different myeloblastic stimulator, this facilitation was not observed. At higher monocyte-lymphocyte ratios (1:15) the monocytes appeared to be capable of strongly inhibiting the MLR. Monocyte capacity to engulf and kill Candida albicans organisms was normal in acute myeloid leukemia (AML) patients given 'immunotherapy" with BCG and leukemic cells. |
| doi_str_mv | 10.3109/08820137909050043 |
| format | Article |
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L.</creatorcontrib><creatorcontrib>Penchansky, Marta</creatorcontrib><creatorcontrib>Lehtinen, Toini</creatorcontrib><creatorcontrib>Reizenstein, Peter G.</creatorcontrib><title>The Role of Monocytes in Phagocytosis and Mixed Leukocyte Reactivity in Human Acute Myeloid Leukemia</title><title>Immunological communications</title><addtitle>Immunol Commun</addtitle><description>Lymphocyte proliferation, as measured by incorporation of tritiated thymidine (3H-TdR), was significantly enhanced (p < 0.01) when macrophages sensitized by target myeloblasts were added to monocyte-depleted lymphocyte fractions in the mixed leukocyte reaction (MLR) with human leukemic myeloblasts as stimulators and panels of normal lymphocytes as responders. Monocyte addition in the same concentration range to unfractionated lymphocytes resulted in highly significant facilitation (p < 0.0001) of MLR response patterns to myeloblastic stimulation. However, with substitution of a different myeloblastic stimulator, this facilitation was not observed. At higher monocyte-lymphocyte ratios (1:15) the monocytes appeared to be capable of strongly inhibiting the MLR. Monocyte capacity to engulf and kill Candida albicans organisms was normal in acute myeloid leukemia (AML) patients given 'immunotherapy" with BCG and leukemic cells.</description><subject>Cell Separation</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Humans</subject><subject>Leukemia, Myeloid - drug therapy</subject><subject>Leukemia, Myeloid - immunology</subject><subject>Leukemia, Myeloid - therapy</subject><subject>Lymphocyte Activation</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Male</subject><subject>Monocytes - immunology</subject><subject>Phagocytosis</subject><issn>0882-0139</issn><issn>0090-0877</issn><issn>1532-4311</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYP4Gh8_QHCRlbtqMklsim5EfMEMiui63ElvnWjbaNKq_femjiAiyl2Ey_nO4eYQssPZvuAsO2BajxkXacYyphiTYomMuBLjRArOl8lo0JMIZOtkI4RHxphQh9kaWeVKHUo1IsXdHOmtq5C6kk5d40zfYqC2oTdzeBg2F2yg0BR0at-xoBPsnj4heotgWvtq237AL7saGnpiuqhMe6ycXbBYW9giKyVUAbe_3k1yf352d3qZTK4vrk5PJomRQrYJ16bUMyUzOTaZKuKJWMiUZQakNqi1LjRoBXGETE2ZzngKJkUsdfwVKik2yd4i99m7lw5Dm9c2GKwqaNB1IU-lEkJqEUG-AI13IXgs82dva_B9zlk-FJv_KjZ6dr_Cu1mNxbfjs8koHy9k25TO1_DmfFXkLfSV86WHxtgwJP-dfvTDPkeo2rkBj_mj63wTa_vntg_Wo5hp</recordid><startdate>1979</startdate><enddate>1979</enddate><creator>Miale, Thomas D.</creator><creator>Stenke, Leif Å. 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L.</creatorcontrib><creatorcontrib>Penchansky, Marta</creatorcontrib><creatorcontrib>Lehtinen, Toini</creatorcontrib><creatorcontrib>Reizenstein, Peter G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunological communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miale, Thomas D.</au><au>Stenke, Leif Å. 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Monocyte addition in the same concentration range to unfractionated lymphocytes resulted in highly significant facilitation (p < 0.0001) of MLR response patterns to myeloblastic stimulation. However, with substitution of a different myeloblastic stimulator, this facilitation was not observed. At higher monocyte-lymphocyte ratios (1:15) the monocytes appeared to be capable of strongly inhibiting the MLR. Monocyte capacity to engulf and kill Candida albicans organisms was normal in acute myeloid leukemia (AML) patients given 'immunotherapy" with BCG and leukemic cells.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>155645</pmid><doi>10.3109/08820137909050043</doi><tpages>23</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-0139 |
| ispartof | Immunological communications, 1979, Vol.8 (3), p.279-301 |
| issn | 0882-0139 0090-0877 1532-4311 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Cell Separation Dose-Response Relationship, Immunologic Humans Leukemia, Myeloid - drug therapy Leukemia, Myeloid - immunology Leukemia, Myeloid - therapy Lymphocyte Activation Lymphocyte Culture Test, Mixed Male Monocytes - immunology Phagocytosis |
| title | The Role of Monocytes in Phagocytosis and Mixed Leukocyte Reactivity in Human Acute Myeloid Leukemia |
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