Association of histamine with cerebral microvessels in regions of bovine brain
Intracerebral blood vessels of diameter< 100 μm (microvessels) were isolated from homogenates of bovine brain regions by a sieving procedure which yielded a preparation suitable for biochemical analysis. It was found that microvessels isolated from cerebral cortex grey matter had levels of endoge...
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| Veröffentlicht in: | Brain research 1979-05, Vol.168 (2), p.323-330 |
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| creator | Jarrott, B. Hjelle, J.T. Spector, S. |
| description | Intracerebral blood vessels of diameter< 100 μm (microvessels) were isolated from homogenates of bovine brain regions by a sieving procedure which yielded a preparation suitable for biochemical analysis. It was found that microvessels isolated from cerebral cortex grey matter had levels of endogenous histamine which were approximately 23-fold greater than the concentration found in the initial homogenate. Microvessels isolated from the striatum and hippocampus also had concentrations of endogenous histamine which were 5- to 6-fold greater than the initial homogenate. It was calculated that approximately half of the endogenous histamine in bovine cerebral cortex grey matter and one tenth of the endogenous histamine in striatum and hippocampus was associated with the microvasculature.
The results of biochemical analysis of microvessel preparations from the cerebral cortex grey matter indicated that: (1) most of endogenous histamine was recovered in the crude nuclear pellet after subcellular fractionation of homogenates; and (2) the endogenous level of histamine was associated with minimal histidine decar☐ylase activity. The above features are characteristic of most cells and it is speculated that histamine in these microvessels was localized in mast cells. |
| doi_str_mv | 10.1016/0006-8993(79)90173-2 |
| format | Article |
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The results of biochemical analysis of microvessel preparations from the cerebral cortex grey matter indicated that: (1) most of endogenous histamine was recovered in the crude nuclear pellet after subcellular fractionation of homogenates; and (2) the endogenous level of histamine was associated with minimal histidine decar☐ylase activity. The above features are characteristic of most cells and it is speculated that histamine in these microvessels was localized in mast cells.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(79)90173-2</identifier><identifier>PMID: 445145</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cattle ; Cerebral Cortex - blood supply ; Cerebral Cortex - enzymology ; Cerebrovascular Circulation ; Corpus Striatum - blood supply ; Corpus Striatum - enzymology ; Hippocampus - blood supply ; Hippocampus - enzymology ; Histamine - metabolism ; Histidine Decarboxylase - metabolism ; Microcirculation - enzymology ; Subcellular Fractions - enzymology</subject><ispartof>Brain research, 1979-05, Vol.168 (2), p.323-330</ispartof><rights>1979 Elsevier/North-Holland Biomedical Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-1c166dbf39bcc973c299409346992d86075e5048763cca11308d2bf48e562aba3</citedby><cites>FETCH-LOGICAL-c356t-1c166dbf39bcc973c299409346992d86075e5048763cca11308d2bf48e562aba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(79)90173-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/445145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jarrott, B.</creatorcontrib><creatorcontrib>Hjelle, J.T.</creatorcontrib><creatorcontrib>Spector, S.</creatorcontrib><title>Association of histamine with cerebral microvessels in regions of bovine brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Intracerebral blood vessels of diameter< 100 μm (microvessels) were isolated from homogenates of bovine brain regions by a sieving procedure which yielded a preparation suitable for biochemical analysis. It was found that microvessels isolated from cerebral cortex grey matter had levels of endogenous histamine which were approximately 23-fold greater than the concentration found in the initial homogenate. Microvessels isolated from the striatum and hippocampus also had concentrations of endogenous histamine which were 5- to 6-fold greater than the initial homogenate. It was calculated that approximately half of the endogenous histamine in bovine cerebral cortex grey matter and one tenth of the endogenous histamine in striatum and hippocampus was associated with the microvasculature.
The results of biochemical analysis of microvessel preparations from the cerebral cortex grey matter indicated that: (1) most of endogenous histamine was recovered in the crude nuclear pellet after subcellular fractionation of homogenates; and (2) the endogenous level of histamine was associated with minimal histidine decar☐ylase activity. The above features are characteristic of most cells and it is speculated that histamine in these microvessels was localized in mast cells.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cerebral Cortex - blood supply</subject><subject>Cerebral Cortex - enzymology</subject><subject>Cerebrovascular Circulation</subject><subject>Corpus Striatum - blood supply</subject><subject>Corpus Striatum - enzymology</subject><subject>Hippocampus - blood supply</subject><subject>Hippocampus - enzymology</subject><subject>Histamine - metabolism</subject><subject>Histidine Decarboxylase - metabolism</subject><subject>Microcirculation - enzymology</subject><subject>Subcellular Fractions - enzymology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS3EqxT-oIusECwCfsWJN0hVxUuqYANry3Em1CiJi50W8fc4pOqS1Wg0516NDkIzgm8IJuIWYyzSQkp2lctriUnOUnqAJqTIaSoox4doskdO0VkIn3FlTOITdMx5Rng2QS_zEJyxureuS1ydrGzodWs7SL5tv0oMeCi9bpLWGu-2EAI0IbFd4uEjJsIQKd124CNmu3N0VOsmwMVuTtH7w_3b4ildvj4-L-bL1LBM9CkxRIiqrJksjZE5M1RKjiXjQkpaFQLnGWSYF7lgxmhCGC4qWta8gExQXWo2RZdj79q7rw2EXrU2GGga3YHbBJXzLArIcAT5CMb3Q_BQq7W3rfY_imA1WFSDIjUoUrlUfxYVjbHZrn9TtlDtQ6O2eL4bz9EGbC14FYyFzkBlPZheVc7-3_8LB2SAoQ</recordid><startdate>19790525</startdate><enddate>19790525</enddate><creator>Jarrott, B.</creator><creator>Hjelle, J.T.</creator><creator>Spector, S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19790525</creationdate><title>Association of histamine with cerebral microvessels in regions of bovine brain</title><author>Jarrott, B. ; Hjelle, J.T. ; Spector, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-1c166dbf39bcc973c299409346992d86075e5048763cca11308d2bf48e562aba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cerebral Cortex - blood supply</topic><topic>Cerebral Cortex - enzymology</topic><topic>Cerebrovascular Circulation</topic><topic>Corpus Striatum - blood supply</topic><topic>Corpus Striatum - enzymology</topic><topic>Hippocampus - blood supply</topic><topic>Hippocampus - enzymology</topic><topic>Histamine - metabolism</topic><topic>Histidine Decarboxylase - metabolism</topic><topic>Microcirculation - enzymology</topic><topic>Subcellular Fractions - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jarrott, B.</creatorcontrib><creatorcontrib>Hjelle, J.T.</creatorcontrib><creatorcontrib>Spector, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jarrott, B.</au><au>Hjelle, J.T.</au><au>Spector, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of histamine with cerebral microvessels in regions of bovine brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1979-05-25</date><risdate>1979</risdate><volume>168</volume><issue>2</issue><spage>323</spage><epage>330</epage><pages>323-330</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>Intracerebral blood vessels of diameter< 100 μm (microvessels) were isolated from homogenates of bovine brain regions by a sieving procedure which yielded a preparation suitable for biochemical analysis. It was found that microvessels isolated from cerebral cortex grey matter had levels of endogenous histamine which were approximately 23-fold greater than the concentration found in the initial homogenate. Microvessels isolated from the striatum and hippocampus also had concentrations of endogenous histamine which were 5- to 6-fold greater than the initial homogenate. It was calculated that approximately half of the endogenous histamine in bovine cerebral cortex grey matter and one tenth of the endogenous histamine in striatum and hippocampus was associated with the microvasculature.
The results of biochemical analysis of microvessel preparations from the cerebral cortex grey matter indicated that: (1) most of endogenous histamine was recovered in the crude nuclear pellet after subcellular fractionation of homogenates; and (2) the endogenous level of histamine was associated with minimal histidine decar☐ylase activity. The above features are characteristic of most cells and it is speculated that histamine in these microvessels was localized in mast cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>445145</pmid><doi>10.1016/0006-8993(79)90173-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Brain research, 1979-05, Vol.168 (2), p.323-330 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Cattle Cerebral Cortex - blood supply Cerebral Cortex - enzymology Cerebrovascular Circulation Corpus Striatum - blood supply Corpus Striatum - enzymology Hippocampus - blood supply Hippocampus - enzymology Histamine - metabolism Histidine Decarboxylase - metabolism Microcirculation - enzymology Subcellular Fractions - enzymology |
| title | Association of histamine with cerebral microvessels in regions of bovine brain |
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