Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy
The influence of the membrane‐stablizing agents diphenylhydantoin (DPH) and orgotein (a drug with superoxide dismutase activity) on protein synthesis was studied in cultured human muscle cells obtained from 7 patients with Duchenne muscular dystrophy (DMD) and 14 controls. The cultures were obtained...
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| Veröffentlicht in: | Annals of neurology 1979-02, Vol.5 (2), p.107-110 |
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| container_title | Annals of neurology |
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| creator | Ionasescu, Victor Stern, Lawrence Z. Ionasescu, Rebeca Rubenstein, Peter |
| description | The influence of the membrane‐stablizing agents diphenylhydantoin (DPH) and orgotein (a drug with superoxide dismutase activity) on protein synthesis was studied in cultured human muscle cells obtained from 7 patients with Duchenne muscular dystrophy (DMD) and 14 controls. The cultures were obtained by dissociation and subsequent plating of cells from biopsied quadriceps muscle. These cultures were then labeled with tritiated leucine in the presence and absence of the membrane‐stabilizing drugs. Total protein synthesis (cpm/mg noncollagen protein) in first‐passage muscle cell cultures from DMD patients showed a 35% decrease, but myosin synthesis revealed normal values. DPH and orgotein increased total protein synthesis by 35 and 50%, respectively, in dystrophic cultured cells, but only by 7 and 8%, respectively, in control cultured cells. |
| doi_str_mv | 10.1002/ana.410050202 |
| format | Article |
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The cultures were obtained by dissociation and subsequent plating of cells from biopsied quadriceps muscle. These cultures were then labeled with tritiated leucine in the presence and absence of the membrane‐stabilizing drugs. Total protein synthesis (cpm/mg noncollagen protein) in first‐passage muscle cell cultures from DMD patients showed a 35% decrease, but myosin synthesis revealed normal values. DPH and orgotein increased total protein synthesis by 35 and 50%, respectively, in dystrophic cultured cells, but only by 7 and 8%, respectively, in control cultured cells.</description><identifier>ISSN: 0364-5134</identifier><identifier>EISSN: 1531-8249</identifier><identifier>DOI: 10.1002/ana.410050202</identifier><identifier>PMID: 426472</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Cells, Cultured ; Child ; Child, Preschool ; Humans ; Metalloproteins - pharmacology ; Muscle Proteins - biosynthesis ; Muscles - drug effects ; Muscles - metabolism ; Muscular Dystrophies - metabolism ; Phenytoin - pharmacology ; Stimulation, Chemical</subject><ispartof>Annals of neurology, 1979-02, Vol.5 (2), p.107-110</ispartof><rights>Copyright © 1979 American Neurological Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3952-3d8316996cd2373564c5478065dfbc184e317f7c1d57dce7a36c98371a1e86fa3</citedby><cites>FETCH-LOGICAL-c3952-3d8316996cd2373564c5478065dfbc184e317f7c1d57dce7a36c98371a1e86fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fana.410050202$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fana.410050202$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/426472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ionasescu, Victor</creatorcontrib><creatorcontrib>Stern, Lawrence Z.</creatorcontrib><creatorcontrib>Ionasescu, Rebeca</creatorcontrib><creatorcontrib>Rubenstein, Peter</creatorcontrib><title>Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy</title><title>Annals of neurology</title><addtitle>Ann Neurol</addtitle><description>The influence of the membrane‐stablizing agents diphenylhydantoin (DPH) and orgotein (a drug with superoxide dismutase activity) on protein synthesis was studied in cultured human muscle cells obtained from 7 patients with Duchenne muscular dystrophy (DMD) and 14 controls. The cultures were obtained by dissociation and subsequent plating of cells from biopsied quadriceps muscle. These cultures were then labeled with tritiated leucine in the presence and absence of the membrane‐stabilizing drugs. Total protein synthesis (cpm/mg noncollagen protein) in first‐passage muscle cell cultures from DMD patients showed a 35% decrease, but myosin synthesis revealed normal values. DPH and orgotein increased total protein synthesis by 35 and 50%, respectively, in dystrophic cultured cells, but only by 7 and 8%, respectively, in control cultured cells.</description><subject>Cells, Cultured</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Humans</subject><subject>Metalloproteins - pharmacology</subject><subject>Muscle Proteins - biosynthesis</subject><subject>Muscles - drug effects</subject><subject>Muscles - metabolism</subject><subject>Muscular Dystrophies - metabolism</subject><subject>Phenytoin - pharmacology</subject><subject>Stimulation, Chemical</subject><issn>0364-5134</issn><issn>1531-8249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtvFDEUhS0EhE2gTEfhim4Se_yaKZclbJCipAgIicY49jU7yTw2fijMv4-jXa2oqGzpfPfo6EPolJIzSkh9bkZzxstPkJrUr9CCCkarpubta7QgTPJKUMbfoeMY7wkhraTkCL3lteSqXqDft6kbcm_SFGYM3oNNEU8eu5D_ROyngLdhStCNOM5j2kDsSjziIUfbA7bQ99jmPuUAERfIZbuBcQTs5pjCtN3M79Ebb_oIH_bvCfrx9eL76rK6ull_Wy2vKstaUVfMNYzKtpXW1UwxIbkVXDVECufvLG04MKq8stQJ5Swow6RtG6aoodBIb9gJ-rTrLXsfM8Skhy6-7DMjTDlqxYsGJlkBqx1owxRjAK-3oRtMmDUl-kWoLkL1QWjhP-6L890A7kDvDJZY7eKnrof5_116eb38t3g_pIsJ_h4uTXjQUjEl9M_rtf61-rL6LNdr3bJnuDmQ6g</recordid><startdate>197902</startdate><enddate>197902</enddate><creator>Ionasescu, Victor</creator><creator>Stern, Lawrence Z.</creator><creator>Ionasescu, Rebeca</creator><creator>Rubenstein, Peter</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197902</creationdate><title>Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy</title><author>Ionasescu, Victor ; Stern, Lawrence Z. ; Ionasescu, Rebeca ; Rubenstein, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3952-3d8316996cd2373564c5478065dfbc184e317f7c1d57dce7a36c98371a1e86fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Cells, Cultured</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Humans</topic><topic>Metalloproteins - pharmacology</topic><topic>Muscle Proteins - biosynthesis</topic><topic>Muscles - drug effects</topic><topic>Muscles - metabolism</topic><topic>Muscular Dystrophies - metabolism</topic><topic>Phenytoin - pharmacology</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ionasescu, Victor</creatorcontrib><creatorcontrib>Stern, Lawrence Z.</creatorcontrib><creatorcontrib>Ionasescu, Rebeca</creatorcontrib><creatorcontrib>Rubenstein, Peter</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ionasescu, Victor</au><au>Stern, Lawrence Z.</au><au>Ionasescu, Rebeca</au><au>Rubenstein, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy</atitle><jtitle>Annals of neurology</jtitle><addtitle>Ann Neurol</addtitle><date>1979-02</date><risdate>1979</risdate><volume>5</volume><issue>2</issue><spage>107</spage><epage>110</epage><pages>107-110</pages><issn>0364-5134</issn><eissn>1531-8249</eissn><abstract>The influence of the membrane‐stablizing agents diphenylhydantoin (DPH) and orgotein (a drug with superoxide dismutase activity) on protein synthesis was studied in cultured human muscle cells obtained from 7 patients with Duchenne muscular dystrophy (DMD) and 14 controls. The cultures were obtained by dissociation and subsequent plating of cells from biopsied quadriceps muscle. These cultures were then labeled with tritiated leucine in the presence and absence of the membrane‐stabilizing drugs. Total protein synthesis (cpm/mg noncollagen protein) in first‐passage muscle cell cultures from DMD patients showed a 35% decrease, but myosin synthesis revealed normal values. DPH and orgotein increased total protein synthesis by 35 and 50%, respectively, in dystrophic cultured cells, but only by 7 and 8%, respectively, in control cultured cells.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>426472</pmid><doi>10.1002/ana.410050202</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0364-5134 |
| ispartof | Annals of neurology, 1979-02, Vol.5 (2), p.107-110 |
| issn | 0364-5134 1531-8249 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Cells, Cultured Child Child, Preschool Humans Metalloproteins - pharmacology Muscle Proteins - biosynthesis Muscles - drug effects Muscles - metabolism Muscular Dystrophies - metabolism Phenytoin - pharmacology Stimulation, Chemical |
| title | Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy |
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