Isolation and identification of previtamin D3 from the skin of rats exposed to ultraviolet irradiation

Isolation and identification of previtamin D3 from the skin of rats exposed to ultraviolet irradiation

The process of the photolytic activation of vitamin D precursor(s) in the skin has been elucidated by a detailed analysis of the products formed after ultraviolet light exposure. The photolytic product isolated from the skin of rats exposed to ultraviolet irradiation was identified as previtamin D3...

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Veröffentlicht in:Biochemistry (Easton) 1979-03, Vol.18 (6), p.1003-1008
Hauptverfasser: Holick, Michael F, Richtand, Neil M, McNeill, Stephen C, Holick, Sally A, Frommer, Jane E, Henley, John W, Potts, John T
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cholecalciferol - biosynthesis
Cholecalciferol - radiation effects
Chromatography, High Pressure Liquid
Kinetics
Mass Spectrometry
Rats
Skin - metabolism
Skin - radiation effects
Ultraviolet Rays
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container_end_page 1008
container_issue 6
container_start_page 1003
container_title Biochemistry (Easton)
container_volume 18
creator Holick, Michael F
Richtand, Neil M
McNeill, Stephen C
Holick, Sally A
Frommer, Jane E
Henley, John W
Potts, John T
description The process of the photolytic activation of vitamin D precursor(s) in the skin has been elucidated by a detailed analysis of the products formed after ultraviolet light exposure. The photolytic product isolated from the skin of rats exposed to ultraviolet irradiation was identified as previtamin D3 by several criteria including its (a) characteristic ultraviolet absorption spectrum, (b) mass spectrum, and (c) thermal isomerization to vitamin D3, which itself was identified also by mass spectroscopy. Vitamin D3 per se was not formed by ultraviolet irradiation--vitamin D3 arises exclusively from the thermal conversion of previtamin D3. Detectable amounts of lumisterol3 or tachysterol3 were not seen.
doi_str_mv 10.1021/bi00573a011
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subjects Animals
Cholecalciferol - biosynthesis
Cholecalciferol - radiation effects
Chromatography, High Pressure Liquid
Kinetics
Mass Spectrometry
Rats
Skin - metabolism
Skin - radiation effects
Ultraviolet Rays
title Isolation and identification of previtamin D3 from the skin of rats exposed to ultraviolet irradiation
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