Centrifugal separation of cells in sputum

Centrifugal separation of cells in sputum

A centrifugation technique was developed and used to separate fixed cells from the sputa of patients with bronchogenic squamous cell carcinoma and ones with no evidence of cancer. This article presents the relative frequencies of occurrence of five cell types (i.e., leukocytes, macrophages, squamous...

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Veröffentlicht in:The journal of histochemistry and cytochemistry 1979-01, Vol.27 (1), p.7-13
Hauptverfasser: Frost, JK, Pressman, NJ, Albright, CD, Gill, GW, Vansickel, MH
Format: Artikel
Sprache:eng
Schlagworte:
Carcinoma, Bronchogenic - pathology
Carcinoma, Squamous Cell - pathology
Cell Separation - methods
Centrifugation, Density Gradient
Humans
Lung Neoplasms - pathology
Sputum - cytology
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container_issue 1
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container_title The journal of histochemistry and cytochemistry
container_volume 27
creator Frost, JK
Pressman, NJ
Albright, CD
Gill, GW
Vansickel, MH
description A centrifugation technique was developed and used to separate fixed cells from the sputa of patients with bronchogenic squamous cell carcinoma and ones with no evidence of cancer. This article presents the relative frequencies of occurrence of five cell types (i.e., leukocytes, macrophages, squamous, columnar and atypical/cancer) in specimen fractions separated from a discontinuous aqueous Ficoll density gradient. These differential counts show that individual cell types may be selectively collected. Atypical and cancer cells are found at high-density gradient fractions (p congruent to 1.138-1.155 g/ml) with a 10-fold enrichment over unprocessed samples.
doi_str_mv 10.1177/27.1.438506
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subjects Carcinoma, Bronchogenic - pathology
Carcinoma, Squamous Cell - pathology
Cell Separation - methods
Centrifugation, Density Gradient
Humans
Lung Neoplasms - pathology
Sputum - cytology
title Centrifugal separation of cells in sputum
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