Radioimmunoassay of DSIP-like material in rat brain

KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691–695, 1978.—Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring...

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Veröffentlicht in:	Brain research bulletin 1978-11, Vol.3 (6), p.691-695
Hauptverfasser:	Kastin, Abba J., Nissen, Cheryl, Schally, Andrew V., Coy, David H.
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Sprache:	eng
Schlagworte:	Animals Brain Brain Chemistry Delta Sleep-Inducing Peptide - analysis DSIP Peptide Radioimmunoassay Rats Sleep
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creator	Kastin, Abba J. Nissen, Cheryl Schally, Andrew V. Coy, David H.
description	KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691–695, 1978.—Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.
doi_str_mv	10.1016/0361-9230(78)90019-9
format	Article
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J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691–695, 1978.—Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.</description><identifier>ISSN: 0361-9230</identifier><identifier>EISSN: 1873-2747</identifier><identifier>DOI: 10.1016/0361-9230(78)90019-9</identifier><identifier>PMID: 318193</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Brain ; Brain Chemistry ; Delta Sleep-Inducing Peptide - analysis ; DSIP ; Peptide ; Radioimmunoassay ; Rats ; Sleep</subject><ispartof>Brain research bulletin, 1978-11, Vol.3 (6), p.691-695</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-ad7d23fc5e34cfdc127953150aaec3e9b18f9784b4d8954b4831960fd52a01473</citedby><cites>FETCH-LOGICAL-c356t-ad7d23fc5e34cfdc127953150aaec3e9b18f9784b4d8954b4831960fd52a01473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0361-9230(78)90019-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/318193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kastin, Abba J.</creatorcontrib><creatorcontrib>Nissen, Cheryl</creatorcontrib><creatorcontrib>Schally, Andrew V.</creatorcontrib><creatorcontrib>Coy, David H.</creatorcontrib><title>Radioimmunoassay of DSIP-like material in rat brain</title><title>Brain research bulletin</title><addtitle>Brain Res Bull</addtitle><description>KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691–695, 1978.—Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. 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J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691–695, 1978.—Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>318193</pmid><doi>10.1016/0361-9230(78)90019-9</doi><tpages>5</tpages></addata></record>
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source	MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects	Animals Brain Brain Chemistry Delta Sleep-Inducing Peptide - analysis DSIP Peptide Radioimmunoassay Rats Sleep
title	Radioimmunoassay of DSIP-like material in rat brain
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