The electrophoretic desorption of human serum albumin from an affinity chromatography matrix using a preparative desorption cell
Preparative amounts of human serum albumin (10 mg) have been electrophoretically desorbed from Cibacron Blue F3G-A-Sepharose 4B in 85% yield. For this purpose an electrophoretic desorption cell was constructed that was reusable and capable of containing larger quantities of affinity matrix.
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| Veröffentlicht in: | Analytical biochemistry 1979, Vol.92 (1), p.144-146 |
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| container_end_page | 146 |
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| container_issue | 1 |
| container_start_page | 144 |
| container_title | Analytical biochemistry |
| container_volume | 92 |
| creator | Morgan, M.R.A. Slater, N.A. Dean, P.D.G. |
| description | Preparative amounts of human serum albumin (10 mg) have been electrophoretically desorbed from Cibacron Blue F3G-A-Sepharose 4B in 85% yield. For this purpose an electrophoretic desorption cell was constructed that was reusable and capable of containing larger quantities of affinity matrix. |
| doi_str_mv | 10.1016/0003-2697(79)90636-5 |
| format | Article |
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For this purpose an electrophoretic desorption cell was constructed that was reusable and capable of containing larger quantities of affinity matrix.</description><subject>Blood Protein Electrophoresis</subject><subject>Chromatography, Affinity - instrumentation</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Humans</subject><subject>Serum Albumin - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3TAQhS1USm8pb8DCq6osAuM4seMNEkKlRUJiA2vLccbEKImDnaDeXR-9vr0IddXV_J05mvkIOWVwzoCJCwDgRSmU_CbVmQLBRVEfkA0DJQrgoD6QzbvkE_mc0jMAY1UtjsjHqhSlLDfk90OPFAe0SwxzHyIu3tIOU4jz4sNEg6P9OpqJJozrSM3QrqOfqIshFxM1zvnJL1tq-9wxS3iKZu63NKfR_6Jr8tMTNXSOOJtoFv-K_5pbHIYv5NCZIeHJWzwmjzffH65_Fnf3P26vr-4Ky2uxFJifYKIy1mHbiNbW3FqrgPGmbRqOjeuMkGCRCwBVd8o5NLJltZFQKpCcH5Ove985hpcV06JHn3YHmAnDmrSsKlVxqLKw2gttDClFdHqOfjRxqxnoHXe9g6p3ULVU-i93Xee10zf_tR2xe1_ag87jy_0Y84-vHqNO1uNksfMxw9dd8P_3_wMqX5U-</recordid><startdate>1979</startdate><enddate>1979</enddate><creator>Morgan, M.R.A.</creator><creator>Slater, N.A.</creator><creator>Dean, P.D.G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1979</creationdate><title>The electrophoretic desorption of human serum albumin from an affinity chromatography matrix using a preparative desorption cell</title><author>Morgan, M.R.A. ; Slater, N.A. ; Dean, P.D.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-e269164acfeb86bc53ccc90138b883e8fda670ce360095d9ffea7b15a70290733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Blood Protein Electrophoresis</topic><topic>Chromatography, Affinity - instrumentation</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Humans</topic><topic>Serum Albumin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morgan, M.R.A.</creatorcontrib><creatorcontrib>Slater, N.A.</creatorcontrib><creatorcontrib>Dean, P.D.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morgan, M.R.A.</au><au>Slater, N.A.</au><au>Dean, P.D.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The electrophoretic desorption of human serum albumin from an affinity chromatography matrix using a preparative desorption cell</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1979</date><risdate>1979</risdate><volume>92</volume><issue>1</issue><spage>144</spage><epage>146</epage><pages>144-146</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Preparative amounts of human serum albumin (10 mg) have been electrophoretically desorbed from Cibacron Blue F3G-A-Sepharose 4B in 85% yield. 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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1979, Vol.92 (1), p.144-146 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74494304 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Blood Protein Electrophoresis Chromatography, Affinity - instrumentation Electrophoresis, Polyacrylamide Gel Humans Serum Albumin - analysis |
| title | The electrophoretic desorption of human serum albumin from an affinity chromatography matrix using a preparative desorption cell |
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