Ultrastructural aspects of tomato leaves infected by Tomato torrado virus (ToTV) and co-infected by other viruses
Optical and electron microscopy studies were carried out to investigate the cytopathology induced in tomato leaves infected by Tomato torrado virus (ToTV), a new picorna-like virus associated with the 'Torrado' disease. Infected leaves, showing typical Torrado disease symptoms were surveye...
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| Veröffentlicht in: | Plant pathology 2010-04, Vol.59 (2), p.231-239 |
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| creator | Alfaro-Fernández, A Medina, V Córdoba-Sellés, M.C Font, M.I Jornet, J Cebrián, M.C Jordá, C |
| description | Optical and electron microscopy studies were carried out to investigate the cytopathology induced in tomato leaves infected by Tomato torrado virus (ToTV), a new picorna-like virus associated with the 'Torrado' disease. Infected leaves, showing typical Torrado disease symptoms were surveyed in commercial greenhouses in the main tomato production areas of Spain. The effect of the co-infection of ToTV with other viruses which commonly infect tomato crops was also studied. Ultra-thin sections of ToTV-infected tomato leaves did not show a strong cellular alteration. However, crystalline arrays of isometric virus-like particles (VLPs) of 20-30 nm in the inclusion bodies were observed in phloem parenchyma cells of the infected tissues. Tissues co-infected by ToTV and either Tomato chlorosis virus (ToCV) or Pepino mosaic virus (PepMV) presented more severe cellular alterations. The most deleterious consequences for tomato cells were found in triple infections of ToTV, PepMV and Tomato spotted wilt virus (TSWV), where characteristic cell wall overgrowth was distinguishable, together with a large amount of necrotic cells. |
| doi_str_mv | 10.1111/j.1365-3059.2009.02215.x |
| format | Article |
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Infected leaves, showing typical Torrado disease symptoms were surveyed in commercial greenhouses in the main tomato production areas of Spain. The effect of the co-infection of ToTV with other viruses which commonly infect tomato crops was also studied. Ultra-thin sections of ToTV-infected tomato leaves did not show a strong cellular alteration. However, crystalline arrays of isometric virus-like particles (VLPs) of 20-30 nm in the inclusion bodies were observed in phloem parenchyma cells of the infected tissues. Tissues co-infected by ToTV and either Tomato chlorosis virus (ToCV) or Pepino mosaic virus (PepMV) presented more severe cellular alterations. The most deleterious consequences for tomato cells were found in triple infections of ToTV, PepMV and Tomato spotted wilt virus (TSWV), where characteristic cell wall overgrowth was distinguishable, together with a large amount of necrotic cells.</description><identifier>ISSN: 0032-0862</identifier><identifier>EISSN: 1365-3059</identifier><identifier>DOI: 10.1111/j.1365-3059.2009.02215.x</identifier><identifier>CODEN: PLPAAD</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Cell walls ; Chlorosis ; Crops ; cytopathology ; Electron microscopy ; Fundamental and applied biological sciences. Psychology ; Greenhouses ; Inclusion bodies ; Infection ; Isometric ; Leaves ; Lycopersicon esculentum ; mixed infection ; necrosis ; Parenchyma ; Pepino mosaic virus ; Phloem ; Phytopathology. Animal pests. Plant and forest protection ; Picorna-like virus ; synergism ; Tomato spotted wilt virus ; Torrado disease ; Virus-like particles ; Wilt</subject><ispartof>Plant pathology, 2010-04, Vol.59 (2), p.231-239</ispartof><rights>2010 The Authors</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4775-6dec7a543ea6d01e868a1996d1f97e66dc6d47d1239ef0d42cb3da965d2b9b633</citedby><cites>FETCH-LOGICAL-c4775-6dec7a543ea6d01e868a1996d1f97e66dc6d47d1239ef0d42cb3da965d2b9b633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3059.2009.02215.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3059.2009.02215.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22487177$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Alfaro-Fernández, A</creatorcontrib><creatorcontrib>Medina, V</creatorcontrib><creatorcontrib>Córdoba-Sellés, M.C</creatorcontrib><creatorcontrib>Font, M.I</creatorcontrib><creatorcontrib>Jornet, J</creatorcontrib><creatorcontrib>Cebrián, M.C</creatorcontrib><creatorcontrib>Jordá, C</creatorcontrib><title>Ultrastructural aspects of tomato leaves infected by Tomato torrado virus (ToTV) and co-infected by other viruses</title><title>Plant pathology</title><description>Optical and electron microscopy studies were carried out to investigate the cytopathology induced in tomato leaves infected by Tomato torrado virus (ToTV), a new picorna-like virus associated with the 'Torrado' disease. Infected leaves, showing typical Torrado disease symptoms were surveyed in commercial greenhouses in the main tomato production areas of Spain. The effect of the co-infection of ToTV with other viruses which commonly infect tomato crops was also studied. Ultra-thin sections of ToTV-infected tomato leaves did not show a strong cellular alteration. However, crystalline arrays of isometric virus-like particles (VLPs) of 20-30 nm in the inclusion bodies were observed in phloem parenchyma cells of the infected tissues. Tissues co-infected by ToTV and either Tomato chlorosis virus (ToCV) or Pepino mosaic virus (PepMV) presented more severe cellular alterations. The most deleterious consequences for tomato cells were found in triple infections of ToTV, PepMV and Tomato spotted wilt virus (TSWV), where characteristic cell wall overgrowth was distinguishable, together with a large amount of necrotic cells.</description><subject>Biological and medical sciences</subject><subject>Cell walls</subject><subject>Chlorosis</subject><subject>Crops</subject><subject>cytopathology</subject><subject>Electron microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Greenhouses</subject><subject>Inclusion bodies</subject><subject>Infection</subject><subject>Isometric</subject><subject>Leaves</subject><subject>Lycopersicon esculentum</subject><subject>mixed infection</subject><subject>necrosis</subject><subject>Parenchyma</subject><subject>Pepino mosaic virus</subject><subject>Phloem</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Picorna-like virus</subject><subject>synergism</subject><subject>Tomato spotted wilt virus</subject><subject>Torrado disease</subject><subject>Virus-like particles</subject><subject>Wilt</subject><issn>0032-0862</issn><issn>1365-3059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkVtr3DAQhUVpodu0v6GCUpo-2NHNujz0IYReAoEGsttXMSvJrRevtZHsNPvvK9chhD5VLyM435kZ5iCEKalpeWe7mnLZVJw0pmaEmJowRpv6_hlaPQrP0YoQziqiJXuJXuW8I4Q2xugVut30Y4I8psmNU4IeQz4EN2YcWzzGPYwR9wHuQsbd0BYheLw94vWijDEl8BHfdWnK-HQd1z8-Yhg8drF6isfxV0gLFfJr9KKFPoc3D_UEbb58Xl98q66-f728OL-qnFCqqaQPTkEjeADpCQ1aaqDGSE9bo4KU3kkvlKeMm9ASL5jbcg9GNp5tzVZyfoI-LH0PKd5OIY9232UX-h6GEKdslRCK6IbO5Lt_yF2c0lCWs1RJqQURghRKL5RLMecUWntI3R7S0VJi5yjszs4Xt_PF7RyF_RuFvS_W9w8DIDvo2wSD6_KjnzGhFVWqcJ8W7nfXh-N_97fX1-fzr_jfLv4WooWfqczY3DBCOaGalqL5H8UIpgw</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Alfaro-Fernández, A</creator><creator>Medina, V</creator><creator>Córdoba-Sellés, M.C</creator><creator>Font, M.I</creator><creator>Jornet, J</creator><creator>Cebrián, M.C</creator><creator>Jordá, C</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201004</creationdate><title>Ultrastructural aspects of tomato leaves infected by Tomato torrado virus (ToTV) and co-infected by other viruses</title><author>Alfaro-Fernández, A ; Medina, V ; Córdoba-Sellés, M.C ; Font, M.I ; Jornet, J ; Cebrián, M.C ; Jordá, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4775-6dec7a543ea6d01e868a1996d1f97e66dc6d47d1239ef0d42cb3da965d2b9b633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Cell walls</topic><topic>Chlorosis</topic><topic>Crops</topic><topic>cytopathology</topic><topic>Electron microscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Greenhouses</topic><topic>Inclusion bodies</topic><topic>Infection</topic><topic>Isometric</topic><topic>Leaves</topic><topic>Lycopersicon esculentum</topic><topic>mixed infection</topic><topic>necrosis</topic><topic>Parenchyma</topic><topic>Pepino mosaic virus</topic><topic>Phloem</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Picorna-like virus</topic><topic>synergism</topic><topic>Tomato spotted wilt virus</topic><topic>Torrado disease</topic><topic>Virus-like particles</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alfaro-Fernández, A</creatorcontrib><creatorcontrib>Medina, V</creatorcontrib><creatorcontrib>Córdoba-Sellés, M.C</creatorcontrib><creatorcontrib>Font, M.I</creatorcontrib><creatorcontrib>Jornet, J</creatorcontrib><creatorcontrib>Cebrián, M.C</creatorcontrib><creatorcontrib>Jordá, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alfaro-Fernández, A</au><au>Medina, V</au><au>Córdoba-Sellés, M.C</au><au>Font, M.I</au><au>Jornet, J</au><au>Cebrián, M.C</au><au>Jordá, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural aspects of tomato leaves infected by Tomato torrado virus (ToTV) and co-infected by other viruses</atitle><jtitle>Plant pathology</jtitle><date>2010-04</date><risdate>2010</risdate><volume>59</volume><issue>2</issue><spage>231</spage><epage>239</epage><pages>231-239</pages><issn>0032-0862</issn><eissn>1365-3059</eissn><coden>PLPAAD</coden><abstract>Optical and electron microscopy studies were carried out to investigate the cytopathology induced in tomato leaves infected by Tomato torrado virus (ToTV), a new picorna-like virus associated with the 'Torrado' disease. Infected leaves, showing typical Torrado disease symptoms were surveyed in commercial greenhouses in the main tomato production areas of Spain. The effect of the co-infection of ToTV with other viruses which commonly infect tomato crops was also studied. Ultra-thin sections of ToTV-infected tomato leaves did not show a strong cellular alteration. However, crystalline arrays of isometric virus-like particles (VLPs) of 20-30 nm in the inclusion bodies were observed in phloem parenchyma cells of the infected tissues. Tissues co-infected by ToTV and either Tomato chlorosis virus (ToCV) or Pepino mosaic virus (PepMV) presented more severe cellular alterations. The most deleterious consequences for tomato cells were found in triple infections of ToTV, PepMV and Tomato spotted wilt virus (TSWV), where characteristic cell wall overgrowth was distinguishable, together with a large amount of necrotic cells.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><doi>10.1111/j.1365-3059.2009.02215.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Journals; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection) |
| subjects | Biological and medical sciences Cell walls Chlorosis Crops cytopathology Electron microscopy Fundamental and applied biological sciences. Psychology Greenhouses Inclusion bodies Infection Isometric Leaves Lycopersicon esculentum mixed infection necrosis Parenchyma Pepino mosaic virus Phloem Phytopathology. Animal pests. Plant and forest protection Picorna-like virus synergism Tomato spotted wilt virus Torrado disease Virus-like particles Wilt |
| title | Ultrastructural aspects of tomato leaves infected by Tomato torrado virus (ToTV) and co-infected by other viruses |
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