Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation
Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose...
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| Veröffentlicht in: | Toxicology and industrial health 2010-06, Vol.26 (5), p.273-280 |
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| creator | Eken, Ayşe Aydın, Ahmet Erdem, Onur Akay, Cemal Sanal, Hatice Tuba Soykut, Buğra Sayal, Ahmet Somuncu, Ibrahim |
| description | Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation. |
| doi_str_mv | 10.1177/0748233710365693 |
| format | Article |
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The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation.</description><identifier>ISSN: 0748-2337</identifier><identifier>EISSN: 1477-0393</identifier><identifier>DOI: 10.1177/0748233710365693</identifier><identifier>PMID: 20371634</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Adult ; Alcohol ; Carcinogens ; Case-Control Studies ; Chromosomes ; Cytogenetic Analysis - methods ; Female ; Gender ; Humans ; Leukocytes, Mononuclear - radiation effects ; Lymphocytes ; Male ; Micronuclei, Chromosome-Defective - radiation effects ; Micronucleus Tests - methods ; Middle Aged ; Mutation ; Occupational Exposure - adverse effects ; Occupational Exposure - analysis ; Personnel, Hospital ; Radiation ; Radiation, Ionizing ; Radiology Department, Hospital ; Regression Analysis ; Risk Factors ; Sister Chromatid Exchange - radiation effects ; Smoking - blood ; Toxicology</subject><ispartof>Toxicology and industrial health, 2010-06, Vol.26 (5), p.273-280</ispartof><rights>The Author(s) 2010</rights><rights>SAGE Publications © Jun 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-ca37ea215edd3e365b824ba1dde40cba353476c0b8a041be4a7a21f2d3ede02e3</citedby><cites>FETCH-LOGICAL-c461t-ca37ea215edd3e365b824ba1dde40cba353476c0b8a041be4a7a21f2d3ede02e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0748233710365693$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0748233710365693$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20371634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eken, Ayşe</creatorcontrib><creatorcontrib>Aydın, Ahmet</creatorcontrib><creatorcontrib>Erdem, Onur</creatorcontrib><creatorcontrib>Akay, Cemal</creatorcontrib><creatorcontrib>Sanal, Hatice Tuba</creatorcontrib><creatorcontrib>Soykut, Buğra</creatorcontrib><creatorcontrib>Sayal, Ahmet</creatorcontrib><creatorcontrib>Somuncu, Ibrahim</creatorcontrib><title>Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation</title><title>Toxicology and industrial health</title><addtitle>Toxicol Ind Health</addtitle><description>Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation.</description><subject>Adult</subject><subject>Alcohol</subject><subject>Carcinogens</subject><subject>Case-Control Studies</subject><subject>Chromosomes</subject><subject>Cytogenetic Analysis - methods</subject><subject>Female</subject><subject>Gender</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - radiation effects</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Micronuclei, Chromosome-Defective - radiation effects</subject><subject>Micronucleus Tests - methods</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Occupational Exposure - adverse effects</subject><subject>Occupational Exposure - analysis</subject><subject>Personnel, Hospital</subject><subject>Radiation</subject><subject>Radiation, Ionizing</subject><subject>Radiology Department, Hospital</subject><subject>Regression Analysis</subject><subject>Risk Factors</subject><subject>Sister Chromatid Exchange - radiation effects</subject><subject>Smoking - blood</subject><subject>Toxicology</subject><issn>0748-2337</issn><issn>1477-0393</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUtrGzEUhUVoqF2n-6yK6CarafQayV4Wk6SBQDbJetBId2wZ2ZpKGtrJKj-98qMNBLq6iPOdc7k6CF1S8o1Spa6JEnPGuaKEy1ou-BmaUqFURfiCf0DTvVzt9Qn6lNKGECJlzT6iCSPFI7mYotflmMMKdpCdwXqn_ZhcwqHDPUTXryFqj1sfgsV-3PbrYMYMB30dUu9yUVPWXYeDMUOvswslwo8YfvchgcU5YB9-YVseB1fR3YvbrXDU1h3wC3TeaZ_g82nO0PPtzdPyR_XweHe__P5QGSFprozmCjSjNVjLoVzbzploNbUWBDGt5jUXShrSzjURtAWhVaE7VmALhAGfoatjbh_DzwFSbrYuGfBe7yAMqVFCyIWqy6_M0Nd35CYMsdyVGl5LJTgTrEDkCJkYUorQNX10Wx3HhpJm303zvpti-XLKHdot2H-Gv2UUoDoCSa_gbel_A_8AlGSZlw</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Eken, 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eken, Ayşe</au><au>Aydın, Ahmet</au><au>Erdem, Onur</au><au>Akay, Cemal</au><au>Sanal, Hatice Tuba</au><au>Soykut, Buğra</au><au>Sayal, Ahmet</au><au>Somuncu, Ibrahim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation</atitle><jtitle>Toxicology and industrial health</jtitle><addtitle>Toxicol Ind Health</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>26</volume><issue>5</issue><spage>273</spage><epage>280</epage><pages>273-280</pages><issn>0748-2337</issn><eissn>1477-0393</eissn><abstract>Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>20371634</pmid><doi>10.1177/0748233710365693</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Alcohol Carcinogens Case-Control Studies Chromosomes Cytogenetic Analysis - methods Female Gender Humans Leukocytes, Mononuclear - radiation effects Lymphocytes Male Micronuclei, Chromosome-Defective - radiation effects Micronucleus Tests - methods Middle Aged Mutation Occupational Exposure - adverse effects Occupational Exposure - analysis Personnel, Hospital Radiation Radiation, Ionizing Radiology Department, Hospital Regression Analysis Risk Factors Sister Chromatid Exchange - radiation effects Smoking - blood Toxicology |
| title | Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation |
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