Electrophysiological Identification of the Functional Presynaptic Nerve Terminals on an Isolated Single Vasopressin Neurone of the Rat Supraoptic Nucleus

Release of arginine vasopressin (AVP) and oxytocin from magnocellular neurosecretory cells (MNCs) of the supraoptic nucleus (SON) is under the control of glutamate‐dependent excitation and GABA‐dependent inhibition. The possible role of the synaptic terminals attached to SON neurones has been invest...

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Veröffentlicht in:Journal of neuroendocrinology 2010-05, Vol.22 (5), p.413-419
Hauptverfasser: Ohbuchi, T., Yokoyama, T., Fujihara, H., Suzuki, H., Ueta, Y.
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container_end_page 419
container_issue 5
container_start_page 413
container_title Journal of neuroendocrinology
container_volume 22
creator Ohbuchi, T.
Yokoyama, T.
Fujihara, H.
Suzuki, H.
Ueta, Y.
description Release of arginine vasopressin (AVP) and oxytocin from magnocellular neurosecretory cells (MNCs) of the supraoptic nucleus (SON) is under the control of glutamate‐dependent excitation and GABA‐dependent inhibition. The possible role of the synaptic terminals attached to SON neurones has been investigated using whole‐cell patch‐clamp recording in in vitro rat brain slice preparations. Recent evidence has provided new insights into the repercussions of glial environment modifications on the physiology of MNCs at the synaptic level in the SON. In the present study, excitatory glutamatergic and inhibitory GABAergic synaptic inputs were recorded from an isolated single SON neurone cultured for 12 h, using the whole‐cell patch clamp technique. Neurones expressed an AVP‐enhanced green fluorescent protein (eGFP) fusion gene in MNCs. In addition, native synaptic terminals attached to a dissociated AVP‐eGFP neurone were visualised with synaptic vesicle markers. These results suggest that the function of presynaptic nerve terminals may be evaluated directly in a single AVP‐eGFP neurone. These preparations would be helpful in future studies aiming to electrophysiologically distinguish between the functions of synaptic terminals and glial modifications in the SON neurones.
doi_str_mv 10.1111/j.1365-2826.2010.01979.x
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subjects Animals
Arginine Vasopressin - genetics
Arginine Vasopressin - physiology
green fluorescent protein
Green Fluorescent Proteins - genetics
Male
Neurons - physiology
Patch-Clamp Techniques
Presynaptic Terminals - physiology
Rats
Rats, Transgenic
supraoptic nucleus
Supraoptic Nucleus - physiology
vasopressin neurone
whole-cell patch-clamp
title Electrophysiological Identification of the Functional Presynaptic Nerve Terminals on an Isolated Single Vasopressin Neurone of the Rat Supraoptic Nucleus
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