Saliva as a source of HCMV DNA in allogeneic stem cell transplantation patients
Oral Diseases (2010) 16, 210–216 Objective: The purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (HCMV) in allogeneic hematopoietic stem cell transplant patients by real time PCR compared with blood. Materials and methods: Saliva and blood...
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| Veröffentlicht in: | Oral diseases 2010-03, Vol.16 (2), p.210-216 |
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| creator | Correia-Silva, JF Bruna-Romero, O Resende, RG Miranda, LPM Oliveira, FE Costa, FO Xavier, SG Figueiredo-Neves, SP Almeida, HC Bittencourt, H Gomez, RS |
| description | Oral Diseases (2010) 16, 210–216
Objective: The purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (HCMV) in allogeneic hematopoietic stem cell transplant patients by real time PCR compared with blood.
Materials and methods: Saliva and blood samples were sampled weekly in 30 allogeneic hematopoietic stem cell transplant patients until 100 days after transplant. Total genomic DNA, extracted from saliva and whole‐blood samples, was used for HCMV real time PCR. Nonparametric tests were performed, and P value ≤0.05 was considered statistically significant.
Results: Human cytomegalovirus DNA load in saliva showed a high correlation with viral DNA in the blood (R = 0.858; P |
| doi_str_mv | 10.1111/j.1601-0825.2009.01637.x |
| format | Article |
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Objective: The purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (HCMV) in allogeneic hematopoietic stem cell transplant patients by real time PCR compared with blood.
Materials and methods: Saliva and blood samples were sampled weekly in 30 allogeneic hematopoietic stem cell transplant patients until 100 days after transplant. Total genomic DNA, extracted from saliva and whole‐blood samples, was used for HCMV real time PCR. Nonparametric tests were performed, and P value ≤0.05 was considered statistically significant.
Results: Human cytomegalovirus DNA load in saliva showed a high correlation with viral DNA in the blood (R = 0.858; P < 0.0001). Blood DNA levels also correlated with HCMV antigenemia (R = 0.773; P < 0.0001). The HCMV levels in saliva (P = 0.015) and blood (P = 0.008) showed higher levels at the beginning of antiviral treatment, with clear reduction after this period. Saliva showed earlier HCMV reactivation than blood detected by real time PCR and antigenemia assay in 11 out of 22 subjects.
Conclusions: This study shows that the real time PCR test could be useful to identify HCMV DNA in saliva and to monitor patients at risk of cytomegalovirus disease after allogeneic hematopoietic stem cell transplant. However, further studies are necessary to confirm this data.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/j.1601-0825.2009.01637.x</identifier><identifier>PMID: 20374507</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; allogeneic hematopoietic stem-cell transplantation ; Antigens, Viral - analysis ; Antigens, Viral - blood ; Antiviral Agents - therapeutic use ; Child ; Cytomegalovirus - genetics ; Cytomegalovirus - immunology ; Cytomegalovirus - isolation & purification ; Cytomegalovirus Infections - diagnosis ; Cytomegalovirus Infections - drug therapy ; Dentistry ; DNA, Viral - analysis ; DNA, Viral - blood ; Feasibility Studies ; Female ; Follow-Up Studies ; Ganciclovir - therapeutic use ; Hematopoietic Stem Cell Transplantation ; Human cytomegalovirus ; Humans ; Longitudinal Studies ; Male ; Middle Aged ; Phosphoproteins - analysis ; Phosphoproteins - blood ; Polymerase Chain Reaction ; Prospective Studies ; saliva ; Saliva - virology ; Transplantation, Homologous ; Viral Load ; Viral Matrix Proteins - analysis ; Viral Matrix Proteins - blood ; Viremia - virology ; Virus Activation ; Young Adult</subject><ispartof>Oral diseases, 2010-03, Vol.16 (2), p.210-216</ispartof><rights>2010 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4707-97d7d73ced7ecb4525ac78417cafc5669033fe3dac91c84116e4fdb32daeff873</citedby><cites>FETCH-LOGICAL-c4707-97d7d73ced7ecb4525ac78417cafc5669033fe3dac91c84116e4fdb32daeff873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1601-0825.2009.01637.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1601-0825.2009.01637.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20374507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Correia-Silva, JF</creatorcontrib><creatorcontrib>Bruna-Romero, O</creatorcontrib><creatorcontrib>Resende, RG</creatorcontrib><creatorcontrib>Miranda, LPM</creatorcontrib><creatorcontrib>Oliveira, FE</creatorcontrib><creatorcontrib>Costa, FO</creatorcontrib><creatorcontrib>Xavier, SG</creatorcontrib><creatorcontrib>Figueiredo-Neves, SP</creatorcontrib><creatorcontrib>Almeida, HC</creatorcontrib><creatorcontrib>Bittencourt, H</creatorcontrib><creatorcontrib>Gomez, RS</creatorcontrib><title>Saliva as a source of HCMV DNA in allogeneic stem cell transplantation patients</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Oral Diseases (2010) 16, 210–216
Objective: The purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (HCMV) in allogeneic hematopoietic stem cell transplant patients by real time PCR compared with blood.
Materials and methods: Saliva and blood samples were sampled weekly in 30 allogeneic hematopoietic stem cell transplant patients until 100 days after transplant. Total genomic DNA, extracted from saliva and whole‐blood samples, was used for HCMV real time PCR. Nonparametric tests were performed, and P value ≤0.05 was considered statistically significant.
Results: Human cytomegalovirus DNA load in saliva showed a high correlation with viral DNA in the blood (R = 0.858; P < 0.0001). Blood DNA levels also correlated with HCMV antigenemia (R = 0.773; P < 0.0001). The HCMV levels in saliva (P = 0.015) and blood (P = 0.008) showed higher levels at the beginning of antiviral treatment, with clear reduction after this period. Saliva showed earlier HCMV reactivation than blood detected by real time PCR and antigenemia assay in 11 out of 22 subjects.
Conclusions: This study shows that the real time PCR test could be useful to identify HCMV DNA in saliva and to monitor patients at risk of cytomegalovirus disease after allogeneic hematopoietic stem cell transplant. However, further studies are necessary to confirm this data.</description><subject>Adolescent</subject><subject>Adult</subject><subject>allogeneic hematopoietic stem-cell transplantation</subject><subject>Antigens, Viral - analysis</subject><subject>Antigens, Viral - blood</subject><subject>Antiviral Agents - therapeutic use</subject><subject>Child</subject><subject>Cytomegalovirus - genetics</subject><subject>Cytomegalovirus - immunology</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytomegalovirus Infections - diagnosis</subject><subject>Cytomegalovirus Infections - drug therapy</subject><subject>Dentistry</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - blood</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Ganciclovir - therapeutic use</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Human cytomegalovirus</subject><subject>Humans</subject><subject>Longitudinal Studies</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Phosphoproteins - analysis</subject><subject>Phosphoproteins - blood</subject><subject>Polymerase Chain Reaction</subject><subject>Prospective Studies</subject><subject>saliva</subject><subject>Saliva - virology</subject><subject>Transplantation, Homologous</subject><subject>Viral Load</subject><subject>Viral Matrix Proteins - analysis</subject><subject>Viral Matrix Proteins - blood</subject><subject>Viremia - virology</subject><subject>Virus Activation</subject><subject>Young Adult</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u2zAQhIkiRZ04fYWCt5ykkFr-SIceDKexDTg20KRNbwRNrQq5suSKcmK_fag69dnkYRfkzOwCHyGUs5iHc7uOuWI8Ymki44SxLGZcgY73H8jl6eMi9CBFJBP4NSBX3q8Z4zqD5BMZJAy0kExfkuWjrcoXS62nlvpm1zqkTUGn44ef9G4xomVNbVU1v7HG0lHf4YY6rCratbb228rWne3KpqbbULDu_DX5WNjK4-f3OiQ_7r89jafRfDmZjUfzyAnNdJTpPFxwmGt0KyETaZ1OBdfOFk4qlTGAAiG3LuMuvHOFoshXkOQWiyLVMCQ3x9xt2_zdoe_MpvT9ZrbGZueNFkKlIBScpUyUEmdkAqRK8rDbkKRHpWsb71sszLYtN7Y9GM5MT8isTQ_C9CBMT8j8I2T2wfrlfchutcH8ZPyPJAi-HgWvZYWHs4PN8m7Wd8EfHf1loLU_-W37xygNWprnxcQ8TueLxfPDdzOGN-7trXk</recordid><startdate>201003</startdate><enddate>201003</enddate><creator>Correia-Silva, JF</creator><creator>Bruna-Romero, O</creator><creator>Resende, RG</creator><creator>Miranda, LPM</creator><creator>Oliveira, FE</creator><creator>Costa, FO</creator><creator>Xavier, SG</creator><creator>Figueiredo-Neves, SP</creator><creator>Almeida, HC</creator><creator>Bittencourt, H</creator><creator>Gomez, RS</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>201003</creationdate><title>Saliva as a source of HCMV DNA in allogeneic stem cell transplantation patients</title><author>Correia-Silva, JF ; Bruna-Romero, O ; Resende, RG ; Miranda, LPM ; Oliveira, FE ; Costa, FO ; Xavier, SG ; Figueiredo-Neves, SP ; Almeida, HC ; Bittencourt, H ; Gomez, RS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4707-97d7d73ced7ecb4525ac78417cafc5669033fe3dac91c84116e4fdb32daeff873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>allogeneic hematopoietic stem-cell transplantation</topic><topic>Antigens, Viral - analysis</topic><topic>Antigens, Viral - blood</topic><topic>Antiviral Agents - therapeutic use</topic><topic>Child</topic><topic>Cytomegalovirus - genetics</topic><topic>Cytomegalovirus - immunology</topic><topic>Cytomegalovirus - isolation & purification</topic><topic>Cytomegalovirus Infections - diagnosis</topic><topic>Cytomegalovirus Infections - drug therapy</topic><topic>Dentistry</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - blood</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Ganciclovir - therapeutic use</topic><topic>Hematopoietic Stem Cell Transplantation</topic><topic>Human cytomegalovirus</topic><topic>Humans</topic><topic>Longitudinal Studies</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Phosphoproteins - analysis</topic><topic>Phosphoproteins - blood</topic><topic>Polymerase Chain Reaction</topic><topic>Prospective Studies</topic><topic>saliva</topic><topic>Saliva - virology</topic><topic>Transplantation, Homologous</topic><topic>Viral Load</topic><topic>Viral Matrix Proteins - analysis</topic><topic>Viral Matrix Proteins - blood</topic><topic>Viremia - virology</topic><topic>Virus Activation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Correia-Silva, JF</creatorcontrib><creatorcontrib>Bruna-Romero, O</creatorcontrib><creatorcontrib>Resende, RG</creatorcontrib><creatorcontrib>Miranda, LPM</creatorcontrib><creatorcontrib>Oliveira, FE</creatorcontrib><creatorcontrib>Costa, FO</creatorcontrib><creatorcontrib>Xavier, SG</creatorcontrib><creatorcontrib>Figueiredo-Neves, SP</creatorcontrib><creatorcontrib>Almeida, HC</creatorcontrib><creatorcontrib>Bittencourt, H</creatorcontrib><creatorcontrib>Gomez, RS</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Correia-Silva, JF</au><au>Bruna-Romero, O</au><au>Resende, RG</au><au>Miranda, LPM</au><au>Oliveira, FE</au><au>Costa, FO</au><au>Xavier, SG</au><au>Figueiredo-Neves, SP</au><au>Almeida, HC</au><au>Bittencourt, H</au><au>Gomez, RS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saliva as a source of HCMV DNA in allogeneic stem cell transplantation patients</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2010-03</date><risdate>2010</risdate><volume>16</volume><issue>2</issue><spage>210</spage><epage>216</epage><pages>210-216</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Oral Diseases (2010) 16, 210–216
Objective: The purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (HCMV) in allogeneic hematopoietic stem cell transplant patients by real time PCR compared with blood.
Materials and methods: Saliva and blood samples were sampled weekly in 30 allogeneic hematopoietic stem cell transplant patients until 100 days after transplant. Total genomic DNA, extracted from saliva and whole‐blood samples, was used for HCMV real time PCR. Nonparametric tests were performed, and P value ≤0.05 was considered statistically significant.
Results: Human cytomegalovirus DNA load in saliva showed a high correlation with viral DNA in the blood (R = 0.858; P < 0.0001). Blood DNA levels also correlated with HCMV antigenemia (R = 0.773; P < 0.0001). The HCMV levels in saliva (P = 0.015) and blood (P = 0.008) showed higher levels at the beginning of antiviral treatment, with clear reduction after this period. Saliva showed earlier HCMV reactivation than blood detected by real time PCR and antigenemia assay in 11 out of 22 subjects.
Conclusions: This study shows that the real time PCR test could be useful to identify HCMV DNA in saliva and to monitor patients at risk of cytomegalovirus disease after allogeneic hematopoietic stem cell transplant. However, further studies are necessary to confirm this data.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20374507</pmid><doi>10.1111/j.1601-0825.2009.01637.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult allogeneic hematopoietic stem-cell transplantation Antigens, Viral - analysis Antigens, Viral - blood Antiviral Agents - therapeutic use Child Cytomegalovirus - genetics Cytomegalovirus - immunology Cytomegalovirus - isolation & purification Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - drug therapy Dentistry DNA, Viral - analysis DNA, Viral - blood Feasibility Studies Female Follow-Up Studies Ganciclovir - therapeutic use Hematopoietic Stem Cell Transplantation Human cytomegalovirus Humans Longitudinal Studies Male Middle Aged Phosphoproteins - analysis Phosphoproteins - blood Polymerase Chain Reaction Prospective Studies saliva Saliva - virology Transplantation, Homologous Viral Load Viral Matrix Proteins - analysis Viral Matrix Proteins - blood Viremia - virology Virus Activation Young Adult |
| title | Saliva as a source of HCMV DNA in allogeneic stem cell transplantation patients |
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