Chitosan microfiber fabrication using a microfluidic chip and its application to cell cultures
In this study, a poly-methyl-methacrylate (PMMA) microfluidic chip with a 45° cross-junction microchannel is fabricated using a CO 2 laser machine to generate chitosan microfibers. Chitosan solution and sodium tripolyphosphate (STPP) solution were injected into the cross-junction microchannel of the...
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| Veröffentlicht in: | Microfluidics and nanofluidics 2010-01, Vol.8 (1), p.115-121 |
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| creator | Yeh, Chia-Hsien Lin, Po-Wen Lin, Yu-Cheng |
| description | In this study, a poly-methyl-methacrylate (PMMA) microfluidic chip with a 45° cross-junction microchannel is fabricated using a CO
2
laser machine to generate chitosan microfibers. Chitosan solution and sodium tripolyphosphate (STPP) solution were injected into the cross-junction microchannel of the microfluidic chip. The laminar flow of the chitosan solution was generated by hydrodynamic focusing. The diameter of laminar flow, which ranged from 30 to 50 μm, was controlled by changing the ratio between chitosan solution and STPP solution flow rates in the PMMA microfluidic chip. The laminar flow of the chitosan solution was converted into chitosan microfibers with STPP solution via the cross-linking reaction; the diameter of chitosan microfibers was in the range of 50–200 μm. The chitosan microfibers were then coated with collagen for cell cultivation. The results show that the chitosan microfibers provide good growth conditions for cells. They could be used as a scaffold for cell cultures in tissue engineering applications. This novel method has advantages of ease of fabrication, simple and low-cost process. |
| doi_str_mv | 10.1007/s10404-009-0485-7 |
| format | Article |
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2
laser machine to generate chitosan microfibers. Chitosan solution and sodium tripolyphosphate (STPP) solution were injected into the cross-junction microchannel of the microfluidic chip. The laminar flow of the chitosan solution was generated by hydrodynamic focusing. The diameter of laminar flow, which ranged from 30 to 50 μm, was controlled by changing the ratio between chitosan solution and STPP solution flow rates in the PMMA microfluidic chip. The laminar flow of the chitosan solution was converted into chitosan microfibers with STPP solution via the cross-linking reaction; the diameter of chitosan microfibers was in the range of 50–200 μm. The chitosan microfibers were then coated with collagen for cell cultivation. The results show that the chitosan microfibers provide good growth conditions for cells. They could be used as a scaffold for cell cultures in tissue engineering applications. This novel method has advantages of ease of fabrication, simple and low-cost process.</description><identifier>ISSN: 1613-4982</identifier><identifier>EISSN: 1613-4990</identifier><identifier>DOI: 10.1007/s10404-009-0485-7</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Applied sciences ; Biomedical Engineering and Bioengineering ; Engineering ; Engineering Fluid Dynamics ; Exact sciences and technology ; Fabrication ; Fibers and threads ; Flow rates ; Forms of application and semi-finished materials ; Growth conditions ; Laminar flow ; Nanotechnology and Microengineering ; Polymer industry, paints, wood ; Short Communication ; Studies ; Technology of polymers</subject><ispartof>Microfluidics and nanofluidics, 2010-01, Vol.8 (1), p.115-121</ispartof><rights>Springer-Verlag 2009</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-18b435bfad726054b1dcd27ed8b7f9c7c083ca31d6c3554db0ff90b8c9767b7d3</citedby><cites>FETCH-LOGICAL-c444t-18b435bfad726054b1dcd27ed8b7f9c7c083ca31d6c3554db0ff90b8c9767b7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10404-009-0485-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10404-009-0485-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22807867$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Yeh, Chia-Hsien</creatorcontrib><creatorcontrib>Lin, Po-Wen</creatorcontrib><creatorcontrib>Lin, Yu-Cheng</creatorcontrib><title>Chitosan microfiber fabrication using a microfluidic chip and its application to cell cultures</title><title>Microfluidics and nanofluidics</title><addtitle>Microfluid Nanofluid</addtitle><description>In this study, a poly-methyl-methacrylate (PMMA) microfluidic chip with a 45° cross-junction microchannel is fabricated using a CO
2
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| subjects | Analytical Chemistry Applied sciences Biomedical Engineering and Bioengineering Engineering Engineering Fluid Dynamics Exact sciences and technology Fabrication Fibers and threads Flow rates Forms of application and semi-finished materials Growth conditions Laminar flow Nanotechnology and Microengineering Polymer industry, paints, wood Short Communication Studies Technology of polymers |
| title | Chitosan microfiber fabrication using a microfluidic chip and its application to cell cultures |
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