First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis
Two farms in the Belgrade area have experienced serious problems with postpartal metritis. Serological examination of BoHV-4 infection was done using ELISA test and vaginal swabs were used for virus isolation. Average seroprevalence of BoHV-4 in these farms was 84.37%. BoHV-4 isolation was successfu...
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| Veröffentlicht in: | The New microbiologica 2007-01, Vol.30 (1), p.53-57 |
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| container_title | The New microbiologica |
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| creator | Nikolin, Veljko M Donofrio, Gaetano Milosevic, Bratislav Taddei, Simone Radosavljevic, Vladimir Milicevic, Vesna |
| description | Two farms in the Belgrade area have experienced serious problems with postpartal metritis. Serological examination of BoHV-4 infection was done using ELISA test and vaginal swabs were used for virus isolation. Average seroprevalence of BoHV-4 in these farms was 84.37%. BoHV-4 isolation was successful from three vaginal swabs on the MDBK cell line. Rising values of BoHV-4 antibodies were recorded in nine of ten cows with clinical signs of postpartal metritis. PCR and restriction analysis were used for better characterisation and isolate classification. Two isolates showed similarity with MOVAR 33/63 virus type, but one differed in polyrepetitive and other parts of DNA. This was the first isolation and characterisation of BoHV4 from Serbian herds. |
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Serological examination of BoHV-4 infection was done using ELISA test and vaginal swabs were used for virus isolation. Average seroprevalence of BoHV-4 in these farms was 84.37%. BoHV-4 isolation was successful from three vaginal swabs on the MDBK cell line. Rising values of BoHV-4 antibodies were recorded in nine of ten cows with clinical signs of postpartal metritis. PCR and restriction analysis were used for better characterisation and isolate classification. Two isolates showed similarity with MOVAR 33/63 virus type, but one differed in polyrepetitive and other parts of DNA. 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Serological examination of BoHV-4 infection was done using ELISA test and vaginal swabs were used for virus isolation. Average seroprevalence of BoHV-4 in these farms was 84.37%. BoHV-4 isolation was successful from three vaginal swabs on the MDBK cell line. Rising values of BoHV-4 antibodies were recorded in nine of ten cows with clinical signs of postpartal metritis. PCR and restriction analysis were used for better characterisation and isolate classification. Two isolates showed similarity with MOVAR 33/63 virus type, but one differed in polyrepetitive and other parts of DNA. This was the first isolation and characterisation of BoHV4 from Serbian herds.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Bovine herpesvirus 4</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - virology</subject><subject>Disease Outbreaks</subject><subject>Endometritis - veterinary</subject><subject>Endometritis - virology</subject><subject>Female</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesviridae Infections - virology</subject><subject>Herpesvirus 4, Bovine - genetics</subject><subject>Herpesvirus 4, Bovine - immunology</subject><subject>Herpesvirus 4, Bovine - isolation & purification</subject><subject>Polymerase Chain Reaction</subject><subject>Postpartum Period</subject><subject>Pregnancy</subject><subject>Restriction Mapping</subject><subject>Yugoslavia - epidemiology</subject><issn>1121-7138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAMgHMAsWnsL6CceBwqNY8m7REmxpAmceBxrdLE04LapcTp0P49nRhXfLElf_5k-4xMGeMs00yUEzJH_MzHUAXjFb8gE6YFq1TOpsQvfcREXyE23uyox9CaBEjDhjZh73dAtxB7wL2PA1JJbx_C6iOTd3QTQ0fNsevot0_bY-0xhXg4zvYBU29iGjraQYo-ebwk5xvTIsxPeUbel49vi1W2fnl6Xtyvs55LljItuNTMagNsXLGQ3JTaSaul0tIIVyoBtsilEAXkwrpKMSvAucKWDdcw3jsjN7_ePoavATDVnUcLbWt2EAastRxVXI-iGbn-n8w5Z0pVI3h1AoemA1f30XcmHuq_N4ofg8BtBw</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Nikolin, Veljko M</creator><creator>Donofrio, Gaetano</creator><creator>Milosevic, Bratislav</creator><creator>Taddei, Simone</creator><creator>Radosavljevic, Vladimir</creator><creator>Milicevic, Vesna</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>200701</creationdate><title>First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis</title><author>Nikolin, Veljko M ; Donofrio, Gaetano ; Milosevic, Bratislav ; Taddei, Simone ; Radosavljevic, Vladimir ; Milicevic, Vesna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p241t-732471c7ae1196542a87d4c74674a3d863ec504335e03cd961c3edd5c8b27e713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Bovine herpesvirus 4</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - virology</topic><topic>Disease Outbreaks</topic><topic>Endometritis - veterinary</topic><topic>Endometritis - virology</topic><topic>Female</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesviridae Infections - virology</topic><topic>Herpesvirus 4, Bovine - genetics</topic><topic>Herpesvirus 4, Bovine - immunology</topic><topic>Herpesvirus 4, Bovine - isolation & purification</topic><topic>Polymerase Chain Reaction</topic><topic>Postpartum Period</topic><topic>Pregnancy</topic><topic>Restriction Mapping</topic><topic>Yugoslavia - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nikolin, Veljko M</creatorcontrib><creatorcontrib>Donofrio, Gaetano</creatorcontrib><creatorcontrib>Milosevic, Bratislav</creatorcontrib><creatorcontrib>Taddei, Simone</creatorcontrib><creatorcontrib>Radosavljevic, Vladimir</creatorcontrib><creatorcontrib>Milicevic, Vesna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The New microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nikolin, Veljko M</au><au>Donofrio, Gaetano</au><au>Milosevic, Bratislav</au><au>Taddei, Simone</au><au>Radosavljevic, Vladimir</au><au>Milicevic, Vesna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis</atitle><jtitle>The New microbiologica</jtitle><addtitle>New Microbiol</addtitle><date>2007-01</date><risdate>2007</risdate><volume>30</volume><issue>1</issue><spage>53</spage><epage>57</epage><pages>53-57</pages><issn>1121-7138</issn><abstract>Two farms in the Belgrade area have experienced serious problems with postpartal metritis. 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| ispartof | The New microbiologica, 2007-01, Vol.30 (1), p.53-57 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antibodies, Viral - blood Bovine herpesvirus 4 Cattle Cattle Diseases - epidemiology Cattle Diseases - virology Disease Outbreaks Endometritis - veterinary Endometritis - virology Female Herpesviridae Infections - veterinary Herpesviridae Infections - virology Herpesvirus 4, Bovine - genetics Herpesvirus 4, Bovine - immunology Herpesvirus 4, Bovine - isolation & purification Polymerase Chain Reaction Postpartum Period Pregnancy Restriction Mapping Yugoslavia - epidemiology |
| title | First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis |
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