Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples
A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity...
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| Veröffentlicht in: | Archives of virology 2010-06, Vol.155 (6), p.817-823 |
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| creator | Fuller, Chad M Brodd, Lina Irvine, Richard M Alexander, Dennis J Aldous, Elizabeth W |
| description | A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls' eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls' eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples. |
| doi_str_mv | 10.1007/s00705-010-0632-1 |
| format | Article |
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A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls' eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls' eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-010-0632-1</identifier><identifier>PMID: 20428904</identifier><language>eng</language><publisher>Vienna: Vienna : Springer Vienna</publisher><subject>Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Bird Diseases - diagnosis ; Bird Diseases - virology ; Birds ; Chickens ; Fundamental and applied biological sciences. Psychology ; Infectious Diseases ; Medical Microbiology ; Microbiology ; Miscellaneous ; Newcastle Disease - diagnosis ; Newcastle Disease - virology ; Newcastle disease virus ; Newcastle disease virus - genetics ; Newcastle disease virus - isolation & purification ; Oligonucleotide Probes ; Original Article ; Paramyxovirus ; Poultry Diseases - diagnosis ; Poultry Diseases - virology ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Sensitivity and Specificity ; Viral Proteins - genetics ; Virology</subject><ispartof>Archives of virology, 2010-06, Vol.155 (6), p.817-823</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-cdd8e3a0dd719fa35e90f5966057b9f31b38f86551243b83ad0dc9d6203cd1d43</citedby><cites>FETCH-LOGICAL-c456t-cdd8e3a0dd719fa35e90f5966057b9f31b38f86551243b83ad0dc9d6203cd1d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-010-0632-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-010-0632-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22879413$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20428904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fuller, Chad M</creatorcontrib><creatorcontrib>Brodd, Lina</creatorcontrib><creatorcontrib>Irvine, Richard M</creatorcontrib><creatorcontrib>Alexander, Dennis J</creatorcontrib><creatorcontrib>Aldous, Elizabeth W</creatorcontrib><title>Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls' eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls' eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bird Diseases - diagnosis</subject><subject>Bird Diseases - virology</subject><subject>Birds</subject><subject>Chickens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Infectious Diseases</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Newcastle Disease - diagnosis</subject><subject>Newcastle Disease - virology</subject><subject>Newcastle disease virus</subject><subject>Newcastle disease virus - genetics</subject><subject>Newcastle disease virus - isolation & purification</subject><subject>Oligonucleotide Probes</subject><subject>Original Article</subject><subject>Paramyxovirus</subject><subject>Poultry Diseases - diagnosis</subject><subject>Poultry Diseases - virology</subject><subject>Reproducibility of Results</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Sensitivity and Specificity</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk2P0zAQhiMEYsvCD-ACFhLiFJiJY8c5rsqnVAFa2LPl2pPiVb7WTir6F_jVuKSwEge42KOZZ96x_TrLHiO8RIDqVUwLiBwQcpC8yPFOtsIyBaqq1d1sBRzKXElQZ9mDGK8BUoKL-9lZAWWhaihX2Y_XtKd2GDvqJzY0zPRsw3bUEwtk2nzy3THaU4iUT8H00QY_Tn7o2ef1JTMxmgNrhsCmb8QcTWR_1Y5Ce5-0RhNMd_g-7H2YI5sOIzFklx8vmO-ZbX3vrWlZNN3YUnyY3WtMG-nRaT_Prt6--bp-n28-vfuwvtjkthRyyq1zirgB5yqsG8MF1dCIWkoQ1bZuOG65apQUAouSbxU3DpytnSyAW4eu5OfZi0V3DMPNTHHSnY-W2tb0NMxRV2Upsca6-D_JOXIFEhL57C_yephDn66huRQl8hp4gnCBbBhiDNToMfjOhING0EdD9WKoTobqo6EaU8-Tk_C87cj96fjtYAKenwAT02M2ySPr4y1XpL-Q5ieuWLiYSv2Owu0J_zX96dLUmEGbXUjCV18KQA6oBKKS_Cd4CcD3</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Fuller, Chad M</creator><creator>Brodd, Lina</creator><creator>Irvine, Richard M</creator><creator>Alexander, Dennis J</creator><creator>Aldous, Elizabeth W</creator><general>Vienna : Springer Vienna</general><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20100601</creationdate><title>Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples</title><author>Fuller, Chad M ; Brodd, Lina ; Irvine, Richard M ; Alexander, Dennis J ; Aldous, Elizabeth W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-cdd8e3a0dd719fa35e90f5966057b9f31b38f86551243b83ad0dc9d6203cd1d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bird Diseases - diagnosis</topic><topic>Bird Diseases - virology</topic><topic>Birds</topic><topic>Chickens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Infectious Diseases</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Newcastle Disease - diagnosis</topic><topic>Newcastle Disease - virology</topic><topic>Newcastle disease virus</topic><topic>Newcastle disease virus - genetics</topic><topic>Newcastle disease virus - isolation & purification</topic><topic>Oligonucleotide Probes</topic><topic>Original Article</topic><topic>Paramyxovirus</topic><topic>Poultry Diseases - diagnosis</topic><topic>Poultry Diseases - virology</topic><topic>Reproducibility of Results</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Sensitivity and Specificity</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuller, Chad M</creatorcontrib><creatorcontrib>Brodd, Lina</creatorcontrib><creatorcontrib>Irvine, Richard M</creatorcontrib><creatorcontrib>Alexander, Dennis J</creatorcontrib><creatorcontrib>Aldous, Elizabeth W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuller, Chad M</au><au>Brodd, Lina</au><au>Irvine, Richard M</au><au>Alexander, Dennis J</au><au>Aldous, Elizabeth W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><addtitle>Arch Virol</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>155</volume><issue>6</issue><spage>817</spage><epage>823</epage><pages>817-823</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls' eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls' eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.</abstract><cop>Vienna</cop><pub>Vienna : Springer Vienna</pub><pmid>20428904</pmid><doi>10.1007/s00705-010-0632-1</doi><tpages>7</tpages></addata></record> |
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| ispartof | Archives of virology, 2010-06, Vol.155 (6), p.817-823 |
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| subjects | Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Bird Diseases - diagnosis Bird Diseases - virology Birds Chickens Fundamental and applied biological sciences. Psychology Infectious Diseases Medical Microbiology Microbiology Miscellaneous Newcastle Disease - diagnosis Newcastle Disease - virology Newcastle disease virus Newcastle disease virus - genetics Newcastle disease virus - isolation & purification Oligonucleotide Probes Original Article Paramyxovirus Poultry Diseases - diagnosis Poultry Diseases - virology Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - veterinary Sensitivity and Specificity Viral Proteins - genetics Virology |
| title | Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples |
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