Bovine serum albumin partitioning in polyethylene glycol (PEG)/potassium citrate aqueous two-phase systems
The extraction and back-extraction of bovine serum albumin (BSA) have been studied by liquid–liquid extraction with poly(ethylene glycol) (PEG)/potassium citrate aqueous two-phase system (ATPS). In this work, the ATPS was examined with regard to the effects of PEG molecular weight (PEG 1000, 2000, 4...
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| Veröffentlicht in: | Food and bioproducts processing 2010-03, Vol.88 (1), p.40-46 |
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| creator | Lu, Yan-Min Yang, Yan-Zhao Zhao, Xi-Dan Xia, Chuan-Bo |
| description | The extraction and back-extraction of bovine serum albumin (BSA) have been studied by liquid–liquid extraction with poly(ethylene glycol) (PEG)/potassium citrate aqueous two-phase system (ATPS). In this work, the ATPS was examined with regard to the effects of PEG molecular weight (PEG 1000, 2000, 4000 and 6000), PEG and potassium citrate concentration, BSA concentration (
C
BSA) and pH on BSA partition. The pH was found to have significant effects on BSA partition with low molecular weight PEG 1000. The yield of the BSA, 99%, was obtained in the top phase under the following conditions: 19% (w/w) PEG 1000, 20% (w/w) potassium citrate and 0.75
mg/g
C
BSA at pH 7.0 and 30
°C. BSA can be re-extracted to a new citrate phase by decreasing the pH of the system with a 92% yield. The back-extraction not only separates the BSA from the polymer, but also allows the polymer to be recycled. The global yield (
Y
e
+
Y
be) is up to 91%. |
| doi_str_mv | 10.1016/j.fbp.2009.12.002 |
| format | Article |
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C
BSA) and pH on BSA partition. The pH was found to have significant effects on BSA partition with low molecular weight PEG 1000. The yield of the BSA, 99%, was obtained in the top phase under the following conditions: 19% (w/w) PEG 1000, 20% (w/w) potassium citrate and 0.75
mg/g
C
BSA at pH 7.0 and 30
°C. BSA can be re-extracted to a new citrate phase by decreasing the pH of the system with a 92% yield. The back-extraction not only separates the BSA from the polymer, but also allows the polymer to be recycled. The global yield (
Y
e
+
Y
be) is up to 91%.</description><identifier>ISSN: 0960-3085</identifier><identifier>EISSN: 1744-3571</identifier><identifier>DOI: 10.1016/j.fbp.2009.12.002</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Aqueous two-phase systems ; Bovine serum albumin ; Partitioning ; Recycling</subject><ispartof>Food and bioproducts processing, 2010-03, Vol.88 (1), p.40-46</ispartof><rights>2009 The Institution of Chemical Engineers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-7a2d9043afdf31e4d7d1bb636d8c4d8d437cfa975e579eef319f6a7c4d30b7353</citedby><cites>FETCH-LOGICAL-c329t-7a2d9043afdf31e4d7d1bb636d8c4d8d437cfa975e579eef319f6a7c4d30b7353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S096030850900100X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Lu, Yan-Min</creatorcontrib><creatorcontrib>Yang, Yan-Zhao</creatorcontrib><creatorcontrib>Zhao, Xi-Dan</creatorcontrib><creatorcontrib>Xia, Chuan-Bo</creatorcontrib><title>Bovine serum albumin partitioning in polyethylene glycol (PEG)/potassium citrate aqueous two-phase systems</title><title>Food and bioproducts processing</title><description>The extraction and back-extraction of bovine serum albumin (BSA) have been studied by liquid–liquid extraction with poly(ethylene glycol) (PEG)/potassium citrate aqueous two-phase system (ATPS). In this work, the ATPS was examined with regard to the effects of PEG molecular weight (PEG 1000, 2000, 4000 and 6000), PEG and potassium citrate concentration, BSA concentration (
C
BSA) and pH on BSA partition. The pH was found to have significant effects on BSA partition with low molecular weight PEG 1000. The yield of the BSA, 99%, was obtained in the top phase under the following conditions: 19% (w/w) PEG 1000, 20% (w/w) potassium citrate and 0.75
mg/g
C
BSA at pH 7.0 and 30
°C. BSA can be re-extracted to a new citrate phase by decreasing the pH of the system with a 92% yield. The back-extraction not only separates the BSA from the polymer, but also allows the polymer to be recycled. The global yield (
Y
e
+
Y
be) is up to 91%.</description><subject>Aqueous two-phase systems</subject><subject>Bovine serum albumin</subject><subject>Partitioning</subject><subject>Recycling</subject><issn>0960-3085</issn><issn>1744-3571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kDFPwzAQhS0EEqXwA9iyAUNSO07iRExQlYJUCQaYLce-tI6SONhuUf49rsrMdDrde0_vPoRuCU4IJsWiTZp6TFKMq4SkCcbpGZoRlmUxzRk5RzNcFTimuMwv0ZVzLcaYlCSfofbZHPQAkQO77yPR1fteD9EorNdem0EP2-i4m24Cv5s6CNJtN0nTRfcfq_XDYjReOKeDV2pvhYdIfO_B7F3kf0w87oQL2ZPz0LtrdNGIzsHN35yjr5fV5_I13ryv35ZPm1jStPIxE6mqcEZFoxpKIFNMkbouaKFKmalSZZTJRlQsh5xVAEFTNYVg4UZxzWhO5-julDtaE7o4z3vtJHSdGI7FeKBSkLwos6AkJ6W0xjkLDR-t7oWdOMH8iJW3PGDlR6ycpDxgDZ7HkwfCCwcNljupYZCgtAXpuTL6H_cvVRqCqw</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Lu, Yan-Min</creator><creator>Yang, Yan-Zhao</creator><creator>Zhao, Xi-Dan</creator><creator>Xia, Chuan-Bo</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100301</creationdate><title>Bovine serum albumin partitioning in polyethylene glycol (PEG)/potassium citrate aqueous two-phase systems</title><author>Lu, Yan-Min ; Yang, Yan-Zhao ; Zhao, Xi-Dan ; Xia, Chuan-Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-7a2d9043afdf31e4d7d1bb636d8c4d8d437cfa975e579eef319f6a7c4d30b7353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aqueous two-phase systems</topic><topic>Bovine serum albumin</topic><topic>Partitioning</topic><topic>Recycling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Yan-Min</creatorcontrib><creatorcontrib>Yang, Yan-Zhao</creatorcontrib><creatorcontrib>Zhao, Xi-Dan</creatorcontrib><creatorcontrib>Xia, Chuan-Bo</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food and bioproducts processing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Yan-Min</au><au>Yang, Yan-Zhao</au><au>Zhao, Xi-Dan</au><au>Xia, Chuan-Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine serum albumin partitioning in polyethylene glycol (PEG)/potassium citrate aqueous two-phase systems</atitle><jtitle>Food and bioproducts processing</jtitle><date>2010-03-01</date><risdate>2010</risdate><volume>88</volume><issue>1</issue><spage>40</spage><epage>46</epage><pages>40-46</pages><issn>0960-3085</issn><eissn>1744-3571</eissn><abstract>The extraction and back-extraction of bovine serum albumin (BSA) have been studied by liquid–liquid extraction with poly(ethylene glycol) (PEG)/potassium citrate aqueous two-phase system (ATPS). In this work, the ATPS was examined with regard to the effects of PEG molecular weight (PEG 1000, 2000, 4000 and 6000), PEG and potassium citrate concentration, BSA concentration (
C
BSA) and pH on BSA partition. The pH was found to have significant effects on BSA partition with low molecular weight PEG 1000. The yield of the BSA, 99%, was obtained in the top phase under the following conditions: 19% (w/w) PEG 1000, 20% (w/w) potassium citrate and 0.75
mg/g
C
BSA at pH 7.0 and 30
°C. BSA can be re-extracted to a new citrate phase by decreasing the pH of the system with a 92% yield. The back-extraction not only separates the BSA from the polymer, but also allows the polymer to be recycled. The global yield (
Y
e
+
Y
be) is up to 91%.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.fbp.2009.12.002</doi><tpages>7</tpages></addata></record> |
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| ispartof | Food and bioproducts processing, 2010-03, Vol.88 (1), p.40-46 |
| issn | 0960-3085 1744-3571 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_744615684 |
| source | Elsevier ScienceDirect Journals |
| subjects | Aqueous two-phase systems Bovine serum albumin Partitioning Recycling |
| title | Bovine serum albumin partitioning in polyethylene glycol (PEG)/potassium citrate aqueous two-phase systems |
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