Estimation of the efficiency of 2D analysis and bioinformatics search in identification of protein markers for colon tumors

A modification of proteome differential analysis was developed; it comprises initial removal of the cell structural proteins by extraction with buffered saline and protein fractionation by gel filtration with subsequent separation by two-dimensional gel electrophoresis and identification by mass-spe...

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Veröffentlicht in:Molecular biology (New York) 2010-04, Vol.44 (2), p.334-340
Hauptverfasser: Bukurova, Yu. A, Khankin, S. L, Krasnov, G. S, Grigor'eva, E. S, Mashkova, T. D, Lisitsyn, N. A, Karpov, V. L, Beresten', S. F
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Sprache:eng
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