Enzyme, Protein, Carbohydrate, and Phenolic Contaminants in Commercial Tyrosinase Preparations: Potential Problems Affecting Tyrosinase Activity and Inhibition Studies

Commercial mushroom tyrosinase contains other proteins, enzymes, carbohydrates, and phenolic material besides tyrosinase. Carbohydrate and phenolic material comprise a large percentage of the powder resuspensions derived from Agaricus bisporus. Enzyme assays identified the presence of tyrosinase, la...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2008-06, Vol.56 (12), p.4760-4768
Hauptverfasser:	Flurkey, Allison, Cooksey, Jena, Reddy, Akhila, Spoonmore, Kelli, Rescigno, Antonio, Inlow, Jennifer, Flurkey, William H
Format:	Artikel
Sprache:	eng
Schlagworte:	accuracy Agaricus - enzymology Agaricus bisporus amino acid sequences Biological and medical sciences carbohydrate content carbohydrates Carbohydrates - analysis catechol oxidase chemical composition Drug Contamination enzyme activity enzyme inhibitors Enzyme Inhibitors - pharmacology enzymes Enzymes - analysis experimental design Food Chemistry/Biochemistry food contamination Food industries Fundamental and applied biological sciences. Psychology glycosidases inhibitors isozymes molecular sequence data Monophenol Monooxygenase - chemistry Monophenol Monooxygenase - metabolism mushrooms peptides phenolic compounds phenolics Phenols - analysis protein content proteins Proteins - analysis Proteins - chemistry tyrosinase
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