Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: Towards a rapid single-step purification process
A streptavidin derivitised macroporous monolith was developed to enable single‐step capture of chemically biotinylated Moloney Murine Leukaemia Virus (MoMuLV) from crude, unclarified cell culture supernatant. Monoliths were prepared by aqueous cryopolymerisation of acrylamide with N,N″‐methylene‐bis...
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| Veröffentlicht in: | Biotechnology and bioengineering 2005-03, Vol.89 (7), p.783-787 |
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| container_title | Biotechnology and bioengineering |
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| creator | Williams, Sharon L. Eccleston, Mark E. Slater, Nigel K.H. |
| description | A streptavidin derivitised macroporous monolith was developed to enable single‐step capture of chemically biotinylated Moloney Murine Leukaemia Virus (MoMuLV) from crude, unclarified cell culture supernatant. Monoliths were prepared by aqueous cryopolymerisation of acrylamide with N,N″‐methylene‐bis (acrylamide) and glycidyl methacrylate (Arvidsson et al. [2003] J Chrom A 986:275–290). Streptavidin was immobilised to the epoxy functionalised monoliths. Particulate‐containing cell culture supernatant was passed through the monolith without preclarification of the feedstock and adsorption capacities of 2 × 105 cfu/ml of adsorbent were demonstrated (cf. Fractogel streptavidin, at 3.9 × 105 cfu/ml of adsorbent). The specific titre of the recovered fraction was increased by 425‐fold; however, recoveries of less than 8% were achieved. Adsorption of nonbiotinylated MoMuLV on the streptavidin‐coated monolith was not observed. © 2005 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.20382 |
| format | Article |
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Monoliths were prepared by aqueous cryopolymerisation of acrylamide with N,N″‐methylene‐bis (acrylamide) and glycidyl methacrylate (Arvidsson et al. [2003] J Chrom A 986:275–290). Streptavidin was immobilised to the epoxy functionalised monoliths. Particulate‐containing cell culture supernatant was passed through the monolith without preclarification of the feedstock and adsorption capacities of 2 × 105 cfu/ml of adsorbent were demonstrated (cf. Fractogel streptavidin, at 3.9 × 105 cfu/ml of adsorbent). The specific titre of the recovered fraction was increased by 425‐fold; however, recoveries of less than 8% were achieved. Adsorption of nonbiotinylated MoMuLV on the streptavidin‐coated monolith was not observed. © 2005 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.20382</identifier><identifier>PMID: 15643627</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acrylamide ; Acrylamides - chemistry ; Adsorption ; affinity chromatography ; Animals ; Biological and medical sciences ; Biotechnology ; biotin ; Biotinylation ; Cell culture ; Chromatography, Affinity - methods ; Colony-forming cells ; Culture Media ; Feasibility Studies ; Fundamental and applied biological sciences. Psychology ; HeLa Cells ; Humans ; Leukemia ; Methacrylates - chemistry ; Methods. Procedures. Technologies ; Mice ; Moloney Murine leukaemia virus (MoMuLV) ; Moloney murine leukemia virus - isolation & purification ; Moloney murine leukemia virus - metabolism ; monolith ; Murine leukemia virus ; Nanostructures ; NIH 3T3 Cells ; Others ; Polymers ; Purification ; Q1 ; Q2 ; Retroviridae - isolation & purification ; Retroviridae - metabolism ; Retrovirus ; streptavidin ; Streptavidin - metabolism ; Various methods and equipments ; Water - chemistry</subject><ispartof>Biotechnology and bioengineering, 2005-03, Vol.89 (7), p.783-787</ispartof><rights>Copyright © 2005 Wiley Periodicals, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>2005 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4602-188a88117110fd0c6e1abff09e481fa6124820663068fa5edec3850b835d383</citedby><cites>FETCH-LOGICAL-c4602-188a88117110fd0c6e1abff09e481fa6124820663068fa5edec3850b835d383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.20382$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.20382$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16610960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15643627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Williams, Sharon L.</creatorcontrib><creatorcontrib>Eccleston, Mark E.</creatorcontrib><creatorcontrib>Slater, Nigel K.H.</creatorcontrib><title>Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: Towards a rapid single-step purification process</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>A streptavidin derivitised macroporous monolith was developed to enable single‐step capture of chemically biotinylated Moloney Murine Leukaemia Virus (MoMuLV) from crude, unclarified cell culture supernatant. Monoliths were prepared by aqueous cryopolymerisation of acrylamide with N,N″‐methylene‐bis (acrylamide) and glycidyl methacrylate (Arvidsson et al. [2003] J Chrom A 986:275–290). Streptavidin was immobilised to the epoxy functionalised monoliths. Particulate‐containing cell culture supernatant was passed through the monolith without preclarification of the feedstock and adsorption capacities of 2 × 105 cfu/ml of adsorbent were demonstrated (cf. Fractogel streptavidin, at 3.9 × 105 cfu/ml of adsorbent). The specific titre of the recovered fraction was increased by 425‐fold; however, recoveries of less than 8% were achieved. Adsorption of nonbiotinylated MoMuLV on the streptavidin‐coated monolith was not observed. © 2005 Wiley Periodicals, Inc.</description><subject>Acrylamide</subject><subject>Acrylamides - chemistry</subject><subject>Adsorption</subject><subject>affinity chromatography</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>biotin</subject><subject>Biotinylation</subject><subject>Cell culture</subject><subject>Chromatography, Affinity - methods</subject><subject>Colony-forming cells</subject><subject>Culture Media</subject><subject>Feasibility Studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Methacrylates - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Moloney Murine leukaemia virus (MoMuLV)</subject><subject>Moloney murine leukemia virus - isolation & purification</subject><subject>Moloney murine leukemia virus - metabolism</subject><subject>monolith</subject><subject>Murine leukemia virus</subject><subject>Nanostructures</subject><subject>NIH 3T3 Cells</subject><subject>Others</subject><subject>Polymers</subject><subject>Purification</subject><subject>Q1</subject><subject>Q2</subject><subject>Retroviridae - isolation & purification</subject><subject>Retroviridae - metabolism</subject><subject>Retrovirus</subject><subject>streptavidin</subject><subject>Streptavidin - metabolism</subject><subject>Various methods and equipments</subject><subject>Water - chemistry</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EokvhwB9AvgDikHZsJ463t7KiS6WKz5U4WhPHBkM2DrZD2Ts_HMMu9AQna6Rn3vHMQ8hDBicMgJ92Pp9wEIrfIgsGy7YCvoTbZAEAshLNkh-Reyl9LmWrpLxLjlgjayF5uyA_zp3zo887anDKc7Q0OIq08yH7cTdgtj2NNsfwzcc50TDSLZoYphBDKbdhDIPPn7yh2KcQOzvmdEY34Rpjn0pOxMn3NPnx42CrlO1Epzl65w1mX7KmGIxN6T6543BI9sHhPSbvL15sVi-rq9fry9X5VWVqCbxiSqFSjLWMgevBSMuwcw6WtlbMoWS8VhykFCCVw8b21gjVQKdE0wsljsnTfWqZ-nW2KeutT8YOA462LKPbum4KzttCPvkvKdvyn1Y1BXy2B8tJUorW6Sn6LcadZqB_qdFFjf6tprCPDqFzt7X9DXlwUYDHBwCTwcFFHI1PN5yUxa2Ewp3uuWs_2N2_J-rnl5s_o6t9hy8Kvv_twPilrCLaRn94tdYX8o18u2bv9Er8BCEXtmo</recordid><startdate>20050330</startdate><enddate>20050330</enddate><creator>Williams, Sharon L.</creator><creator>Eccleston, Mark E.</creator><creator>Slater, Nigel K.H.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050330</creationdate><title>Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: Towards a rapid single-step purification process</title><author>Williams, Sharon L. ; Eccleston, Mark E. ; Slater, Nigel K.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4602-188a88117110fd0c6e1abff09e481fa6124820663068fa5edec3850b835d383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acrylamide</topic><topic>Acrylamides - chemistry</topic><topic>Adsorption</topic><topic>affinity chromatography</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>biotin</topic><topic>Biotinylation</topic><topic>Cell culture</topic><topic>Chromatography, Affinity - methods</topic><topic>Colony-forming cells</topic><topic>Culture Media</topic><topic>Feasibility Studies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Methacrylates - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Moloney Murine leukaemia virus (MoMuLV)</topic><topic>Moloney murine leukemia virus - isolation & purification</topic><topic>Moloney murine leukemia virus - metabolism</topic><topic>monolith</topic><topic>Murine leukemia virus</topic><topic>Nanostructures</topic><topic>NIH 3T3 Cells</topic><topic>Others</topic><topic>Polymers</topic><topic>Purification</topic><topic>Q1</topic><topic>Q2</topic><topic>Retroviridae - isolation & purification</topic><topic>Retroviridae - metabolism</topic><topic>Retrovirus</topic><topic>streptavidin</topic><topic>Streptavidin - metabolism</topic><topic>Various methods and equipments</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Williams, Sharon L.</creatorcontrib><creatorcontrib>Eccleston, Mark E.</creatorcontrib><creatorcontrib>Slater, Nigel K.H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Williams, Sharon L.</au><au>Eccleston, Mark E.</au><au>Slater, Nigel K.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: Towards a rapid single-step purification process</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2005-03-30</date><risdate>2005</risdate><volume>89</volume><issue>7</issue><spage>783</spage><epage>787</epage><pages>783-787</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>A streptavidin derivitised macroporous monolith was developed to enable single‐step capture of chemically biotinylated Moloney Murine Leukaemia Virus (MoMuLV) from crude, unclarified cell culture supernatant. Monoliths were prepared by aqueous cryopolymerisation of acrylamide with N,N″‐methylene‐bis (acrylamide) and glycidyl methacrylate (Arvidsson et al. [2003] J Chrom A 986:275–290). Streptavidin was immobilised to the epoxy functionalised monoliths. Particulate‐containing cell culture supernatant was passed through the monolith without preclarification of the feedstock and adsorption capacities of 2 × 105 cfu/ml of adsorbent were demonstrated (cf. Fractogel streptavidin, at 3.9 × 105 cfu/ml of adsorbent). The specific titre of the recovered fraction was increased by 425‐fold; however, recoveries of less than 8% were achieved. Adsorption of nonbiotinylated MoMuLV on the streptavidin‐coated monolith was not observed. © 2005 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15643627</pmid><doi>10.1002/bit.20382</doi><tpages>5</tpages></addata></record> |
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| subjects | Acrylamide Acrylamides - chemistry Adsorption affinity chromatography Animals Biological and medical sciences Biotechnology biotin Biotinylation Cell culture Chromatography, Affinity - methods Colony-forming cells Culture Media Feasibility Studies Fundamental and applied biological sciences. Psychology HeLa Cells Humans Leukemia Methacrylates - chemistry Methods. Procedures. Technologies Mice Moloney Murine leukaemia virus (MoMuLV) Moloney murine leukemia virus - isolation & purification Moloney murine leukemia virus - metabolism monolith Murine leukemia virus Nanostructures NIH 3T3 Cells Others Polymers Purification Q1 Q2 Retroviridae - isolation & purification Retroviridae - metabolism Retrovirus streptavidin Streptavidin - metabolism Various methods and equipments Water - chemistry |
| title | Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: Towards a rapid single-step purification process |
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