THE COMPLEX DIAGNOSIS OF CERVICAL INTRAEPITHELIAL NEOPLASIA

The complex diagnosis of the cervical intraepithelial neoplasia requires, nowadays, methods in the field of cytology, colposcopy, molecular diagnosis and pathology in order to get a fine diagnosis and an accurate prognosis. The progress achieved in the ethiopathology of cervical cancer has brought new data which have to be integrated carefully. The aim of this study was the analysis of actual diagnostic methods upon 50 patients that were admitted into the Clinic of Obstetrics and Gynecology, Napoca, Romania diagnosed with intraepithelial neoplasia between 1st of January 2008 and 31 of July 2008. For each patient monolayer cytology and colposcopy were performed. HPV 16 and 18 detection were performed immunohistochemistry using a HPV high risk kit and nested PCR. Biopsies were examined in a pathology laboratory. The maximum concordance between cytology, colposcopy and biopsy appeared in cases of low grade squamous intraepithelial lesion and cervical intraepithelial neoplasia and a discrepancy was found in cases of ASC-H and CIN HI. The molecular methods have their particularities related to the stage of the viral infection that needs to be taken into consideration.